##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212711_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 321437 Sequences flagged as poor quality 0 Sequence length 58 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.66747760836493 38.0 36.0 38.0 33.0 38.0 2 35.269539598739414 38.0 34.0 38.0 29.0 38.0 3 36.33264683281639 38.0 36.0 38.0 33.0 38.0 4 36.19361492298646 38.0 36.0 38.0 32.0 38.0 5 33.13652442002632 35.0 32.0 38.0 25.0 38.0 6 36.89462009662858 38.0 36.0 39.0 33.0 39.0 7 36.96728752446047 38.0 37.0 39.0 34.0 39.0 8 36.85968012394342 38.0 37.0 39.0 33.0 39.0 9 36.780364425999494 38.0 37.0 39.0 33.0 39.0 10 35.27022402523667 38.0 34.0 38.0 29.0 39.0 11 35.80095010841938 38.0 35.0 39.0 30.0 39.0 12 35.56452119699972 38.0 35.0 39.0 30.0 39.0 13 35.432977535255745 38.0 35.0 39.0 29.0 39.0 14 35.39790378830066 38.0 35.0 39.0 29.0 39.0 15 35.44142709146738 38.0 35.0 39.0 29.0 39.0 16 34.964008499332685 38.0 33.0 38.0 29.0 39.0 17 34.80822369546754 37.0 33.0 38.0 29.0 39.0 18 34.6464812700467 37.0 33.0 38.0 28.0 39.0 19 34.19522954731409 36.0 33.0 38.0 27.0 39.0 20 34.28949063113456 36.0 33.0 38.0 27.0 39.0 21 33.9236180029057 36.0 33.0 38.0 26.0 39.0 22 33.79389740446806 36.0 33.0 38.0 26.0 39.0 23 33.54944825891232 36.0 32.0 38.0 26.0 39.0 24 33.40699110556657 36.0 32.0 38.0 25.0 39.0 25 33.398208669194894 36.0 32.0 38.0 26.0 39.0 26 32.778164928119665 36.0 32.0 38.0 23.0 39.0 27 32.683272305303994 35.0 31.0 38.0 23.0 39.0 28 32.11307659043607 35.0 30.0 38.0 22.0 39.0 29 32.215600568696196 35.0 31.0 38.0 22.0 39.0 30 31.708577419525444 35.0 30.0 38.0 19.0 39.0 31 30.773781487507662 35.0 29.0 38.0 14.0 39.0 32 30.380286028055263 35.0 28.0 38.0 12.0 39.0 33 30.3994437479195 35.0 28.0 38.0 13.0 38.0 34 29.716308327914955 34.0 27.0 38.0 10.0 38.0 35 29.28684936706104 33.0 26.0 38.0 8.0 38.0 36 30.392219937343864 35.0 29.0 38.0 2.0 39.0 37 30.19433357080859 35.0 29.0 38.0 2.0 39.0 38 30.047324981256047 35.0 28.0 38.0 2.0 39.0 39 30.011174818082548 35.0 28.0 38.0 2.0 39.0 40 29.792606327211864 35.0 28.0 38.0 2.0 39.0 41 30.13148144115332 35.0 29.0 38.0 2.0 39.0 42 29.84608181385466 35.0 28.0 38.0 2.0 39.0 43 29.682133668494917 35.0 28.0 38.0 2.0 39.0 44 29.54703409999471 35.0 28.0 38.0 2.0 39.0 45 29.22669761104042 35.0 27.0 38.0 2.0 39.0 46 28.624091190497673 35.0 26.0 38.0 2.0 39.0 47 28.464934030618753 34.0 26.0 38.0 2.0 39.0 48 28.47847945320545 34.0 26.0 38.0 2.0 39.0 49 28.162821952668796 34.0 25.0 38.0 2.0 39.0 50 27.918761685804682 34.0 24.0 38.0 2.0 39.0 51 27.493393728786668 34.0 23.0 38.0 2.0 39.0 52 27.267461431011366 34.0 23.0 38.0 2.0 39.0 53 27.068959080628552 33.0 23.0 37.0 2.0 39.0 54 26.776696522180085 33.0 21.0 37.0 2.0 39.0 55 26.501333076154893 33.0 19.0 37.0 2.0 39.0 56 26.135189788356662 34.0 2.0 38.0 2.0 39.0 57 25.681306134639136 33.0 2.0 37.0 2.0 39.0 58 25.241440779997326 33.0 2.0 36.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -1.4914805287282888 1 2 -2.2451163844132083 1 3 -2.2482109661718184 1 4 -2.6984272910171114 1 5 -2.5344585964636153 1 6 -2.010244595726803 1 7 -2.1369287543510183 1 8 -2.4148509528108306 1 9 -2.48528630265465 1 10 -1.930141055139572 1 11 -1.2986596162531612 1 12 -1.9869420548946195 1 13 -1.2708286297782365 1 14 -1.1441637962170077 1 15 -1.7225877225333903 1 16 -1.4791186726407446 1 17 -0.7536144406220586 1 18 -1.4647517716464833 1 19 -0.3670161083784791 1 20 -1.2131966809572887 1 21 -1.1705977925344868 1 22 -0.935700287728892 1 23 -1.7385087582096403 1 24 -0.5529982897278813 1 25 -0.4865292552188265 1 26 -0.9018958671478288 1 27 -1.089384020808815 1 28 -0.5582028224196662 1 29 -0.6090229634611433 1 30 -1.0539178252133752 1 31 -0.8343212056973073 1 32 -1.1475944202651505 1 33 -0.7700897339266177 1 34 -1.1499807896452943 1 35 -1.0980165218778701 1 36 -0.9319855890498836 1 37 -0.913305780068363 1 38 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-0.05212134964551751 117 18 -0.9531501765430761 117 19 -0.17009502890152106 117 20 -0.6501622749478244 117 21 0.08973792108881185 117 22 -0.24390765117134805 117 23 0.04934248912412187 117 24 -0.35442646370543684 117 25 -0.20250811082487274 117 26 0.12127022763098694 117 27 -0.3801750808106732 117 28 0.2012889702168934 117 29 -0.7140772155245756 117 30 -0.2550306880530293 117 31 -0.5622076936876645 117 32 -0.991033166439685 117 33 -0.6094108875944286 117 34 -0.9113273536812763 117 35 -0.4681176069191366 117 36 -0.5485672453607435 117 37 -0.7931165933856228 117 38 -0.9311787922253885 117 39 -0.7563198781053195 117 40 -0.7867693923674075 117 41 -1.1618133479217008 117 42 -0.9332180555197773 117 43 -1.2893679930242818 117 44 -1.0076830133633408 117 45 -0.7109420324048301 117 46 -0.676417142978547 117 47 -0.4502923558041374 117 48 -0.25774027195986093 117 49 -0.44998927858604176 117 50 -0.31739729000793204 117 51 -1.1218742445294119 117 52 -1.5553878210536674 117 53 -1.072309178146707 117 54 -1.7091568921447475 117 55 -1.4770576559477249 117 56 -1.057806537915539 117 57 -1.2384897016076337 117 58 -1.7717276302995053 118 1 -0.6304111103417966 118 2 -0.6982120692162113 118 3 -0.7108751312750101 118 4 -1.1218794486156085 118 5 -0.5065036246062036 118 6 -0.6141845582033554 118 7 -0.5546585854954813 118 8 -0.6454325663192719 118 9 -0.7954176347372055 118 10 -0.7118483722127422 118 11 -0.38289976634697354 118 12 -0.28694205489462377 118 13 -0.7674515190840481 118 14 -0.4860962540031224 118 15 -0.6661149270362046 118 16 -0.34365901035181423 118 17 -0.40042494718866095 118 18 -0.8716936102956367 118 19 -0.2384044761083075 118 20 -0.4459358929647905 118 21 -0.39395614150259206 118 22 -0.5140304941078782 118 23 -0.5701222666524188 118 24 -0.0879889088648369 118 25 0.20427749900256487 118 26 -0.34486021986828064 118 27 -0.5643371164936184 118 28 -0.5950696141645047 118 29 0.12296577950321108 118 30 -0.3878765494160028 118 31 0.1062041226328958 118 32 -0.26260380112818993 118 33 -0.06868260447070895 118 34 0.1597753079156803 118 35 0.04485402221218848 118 36 -0.7887398104382513 118 37 -0.2919174123385311 118 38 -0.882962939636915 118 39 -0.6915787257215804 118 40 -0.27474833357828743 118 41 -0.6345831636572932 118 42 -0.34117359807607883 118 43 -0.4106753928487912 118 44 -0.10018081973947446 118 45 -0.30746148253644634 118 46 -0.09844112659948934 118 47 -0.09066965910919933 118 48 -0.9071991780727586 118 49 0.0369367231688571 118 50 -0.16281992820623614 118 51 -0.2620614337660321 118 52 0.5354720795020498 118 53 -0.28996053819350465 118 54 -0.5988761082898186 118 55 -0.15218196130016892 118 56 -0.5165810333820495 118 57 0.1605743522092702 118 58 0.2541717554858103 119 1 -0.6176516475823348 119 2 -0.43038545138958995 119 3 -0.7826089530088325 119 4 -1.3022213289574935 119 5 -0.2843424524450313 119 6 -0.49452643854523615 119 7 -0.7082605390974379 119 8 -0.5788269497136582 119 9 -0.8551856445052124 119 10 -0.8491499595143281 119 11 0.02735664390944237 119 12 -0.7575769755295383 119 13 -0.273983875616409 119 14 -0.7371951551020217 119 15 -0.23968025060153053 119 16 -0.9729630396558377 119 17 -0.6158095625732756 119 18 -0.7309732195752474 119 19 -0.7697842074880441 119 20 -0.7823217293506275 119 21 -0.4940782416246918 119 22 -0.5554834855608703 119 23 -0.8867278832580325 119 24 -0.7036177244936539 119 25 -0.5286895339644673 119 26 -0.5720274970355561 119 27 -0.5464494486059479 119 28 -0.5904908595857492 119 29 -0.9006605941231633 119 30 -0.6306115921510447 119 31 -0.13012066369189057 119 32 -0.06205435057874098 119 33 0.17429663850853316 119 34 0.06533086347123884 119 35 0.9592618366200014 119 36 0.2028963311978913 119 37 0.4362877158665981 119 38 0.8226536659796935 119 39 1.0494469153040598 119 40 0.5007705919406362 119 41 0.6885548094806779 119 42 0.7602305533280713 119 43 0.7794955473221492 119 44 1.1437141741555195 119 45 1.4794738043988396 119 46 1.0497884216300584 119 47 1.100661232221693 119 48 0.7112379403643594 119 49 0.9251540613861948 119 50 1.5438345284482224 119 51 1.1665710448664477 119 52 1.3835795276094984 119 53 1.1677928195598533 119 54 1.4395243801106687 119 55 1.2480011888829807 119 56 1.6470941802931627 119 57 1.5331018247367432 119 58 1.5312779825920373 120 1 -1.02978316685671 120 2 -1.1219954287138592 120 3 -1.254831175231054 120 4 -1.7166204219280132 120 5 -1.0785601395198583 120 6 -1.4395377764137152 120 7 -1.767217455197212 120 8 -1.985856428171708 120 9 -1.8693579801061233 120 10 -0.8893993926209021 120 11 -0.48454811799532393 120 12 -0.377758381425231 120 13 -0.7428047372944135 120 14 -0.4871951551020217 120 15 -0.5503378469734059 120 16 -0.44915351584631935 120 17 -0.29948303196103154 120 18 -0.2967328567634553 120 19 -0.5611674274426903 120 20 -0.6712106182395132 120 21 -0.9186814162278694 120 22 -0.5730571817059982 120 23 -0.6990861599020235 120 24 -0.7722118288020425 120 25 -0.7786895339644673 120 26 -0.12531547889496863 120 27 -0.1762680427102623 120 28 -0.05817793441568142 120 29 -0.03331365534765496 120 30 0.3807262763296819 120 31 1.1295165245167276 120 32 1.8308027922784014 120 33 1.17032838454028 120 34 1.2750814303646614 120 35 1.5012119499986873 120 36 1.6660482586355307 120 37 1.8155394165468657 120 38 1.2625629630318471 120 39 1.767133990133992 120 40 1.5693646962490249 120 41 1.6738155804557344 120 42 1.3957180816727437 120 43 1.2290420325829174 120 44 1.4594738113437309 120 45 1.7255055504305865 120 46 1.9057974919248437 120 47 1.9181215496820094 120 48 1.7089703666682148 120 49 1.6932946509553588 120 50 1.4423606055457263 120 51 2.105913448494565 120 52 1.4629446069745775 120 53 1.795910733392052 120 54 2.0205901397478563 120 55 1.5915386038489672 120 56 1.5955068787058622 120 57 1.7984079471857228 120 58 1.6707337649049627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 693.0 3 51.0 4 144.0 5 241.0 6 506.0 7 942.0 8 589.0 9 817.0 10 1079.0 11 1196.0 12 1596.0 13 1880.0 14 2222.0 15 2830.0 16 3273.0 17 3434.0 18 4079.0 19 3516.0 20 2790.0 21 2957.0 22 3704.0 23 4416.0 24 4289.0 25 4689.0 26 5783.0 27 7039.0 28 8660.0 29 10624.0 30 12808.0 31 15502.0 32 19456.0 33 23688.0 34 29630.0 35 35585.0 36 40340.0 37 40283.0 38 17567.0 39 2539.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 58.677439124929606 27.44270261357591 4.664055475878633 9.215802785615844 11 32.635633110065115 27.07840105526122 16.442102184876042 23.84386364979763 12 39.7667350056154 13.200098308533242 26.98911450766402 20.04405217818733 13 21.709386287204026 14.565840273521715 31.922274038147446 31.802499401126816 14 21.201355164464577 22.414034476429283 36.57077436636106 19.81383599274508 15 30.2124522068088 13.567199793427639 34.41794192952274 21.802406070240824 16 26.474943690190273 15.993852586518 34.183165544618525 23.348038178673203 17 35.0198329366746 15.609687806243874 22.572837432140247 26.797641824941277 18 33.38922833783397 16.965741236202543 25.83500292437686 23.81002750158663 19 28.359922348461282 19.71030002862156 29.624248684030412 22.305528938886745 20 29.337556116242673 25.154076613643362 23.174491570508135 22.333875699605827 21 28.93733919465885 19.171263327204453 31.307380479048252 20.584016999088444 22 31.66250307214166 18.409517261547364 24.269141386959188 25.658838279351787 23 33.990704099156275 23.959655541452 23.020110007715473 19.02953035167625 24 32.961149342326316 24.176819024626987 21.694831941661793 21.167199691384912 25 29.46247464503043 19.836297116688858 20.07149168108115 30.629736557199568 26 31.96786348828019 17.693750991819627 24.0743794709673 26.26400604893288 27 24.170941979161157 19.119280440795347 22.637118527529488 34.07265905251401 28 26.78497524606131 26.66050540977026 21.657440340051593 24.89707900411684 29 34.21792011649289 19.333339556244226 22.744428161160947 23.704312166101936 30 26.561804606751714 23.172253673232476 26.115819252997873 24.150122467017937 31 35.37637052275153 17.968125134212418 25.12954416016482 21.52596018287123 32 34.58051227161846 16.95406232886942 29.523759147707473 18.94166625180465 33 36.266820154628746 16.175971874368027 26.56472162157958 20.992486349423643 34 29.440975236388418 19.354136136080076 31.53712363759688 19.66776498993463 35 26.442539554131212 28.043995706218205 29.50948210146393 16.003982638186656 36 28.3455377788013 27.879040615440154 25.650808667697795 18.124612938060753 37 28.313208016930165 24.747292418772563 29.63867795344205 17.300821610855223 38 28.294052465792653 25.101781645687822 27.485401959685753 19.118763928833776 39 28.56098092303862 22.82731148663368 27.347275386674134 21.264432203653566 40 26.840169062128467 22.45799901650161 29.002931821152682 21.69890010021724 41 26.91466151255692 21.493197882333167 29.72919137122351 21.862949233886404 42 23.921936094438774 22.469989789056857 29.780153413194533 23.82792070330984 43 26.313380983214625 18.75342431638193 29.672946655376798 25.260248045026646 44 25.95332652756337 18.168604651162788 30.321261391364974 25.55680742990887 45 26.426979720392897 19.78382113112652 30.586540017677745 23.202659130802843 46 25.41730342464261 22.3241380812913 30.788168907121005 21.47038958694509 47 25.11403866572426 21.72448258361019 31.43543932520247 21.726039425463085 48 27.772675050361322 20.144218644431923 30.12587996101862 21.957226344188133 49 25.33188010899183 20.92393927598287 30.96551187232386 22.77866874270144 50 23.11369376270954 19.815830367124757 32.538918836436565 24.53155703372914 51 19.957221182675514 21.258955063561103 34.43601391089926 24.347809842864127 52 22.032700533594006 20.084802221544248 36.47803049642299 21.404466748438754 53 21.115616008370388 20.080527633496505 36.02879812662705 22.775058231506048 54 20.985954968702313 21.06412132913955 34.41281803743266 23.537105664725484 55 21.459655287348802 20.229206189003214 31.82003669541868 26.491101828229308 56 22.31059933390036 21.40429257289554 28.10859480392432 28.176513289279782 57 24.76517740593856 22.017714113032618 29.098063161436443 24.119045319592384 58 24.338571588799024 25.114028638012037 28.58224393405032 21.965155839138617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 88.5 10 177.0 11 225.0 12 273.0 13 282.0 14 291.0 15 350.5 16 797.5 17 1185.0 18 1596.0 19 2007.0 20 1861.0 21 1715.0 22 2059.5 23 2816.0 24 3228.0 25 3862.5 26 4497.0 27 5048.5 28 5823.0 29 6046.0 30 6205.0 31 6364.0 32 6008.5 33 5653.0 34 7816.5 35 7574.0 36 5168.0 37 4812.5 38 4457.0 39 4296.5 40 4136.0 41 4200.5 42 4417.5 43 4570.0 44 5603.0 45 6636.0 46 7773.5 47 8352.0 48 7793.0 49 7633.5 50 7474.0 51 8806.0 52 10138.0 53 11909.5 54 14653.5 55 15626.0 56 16354.5 57 17083.0 58 20008.5 59 24894.0 60 26854.0 61 26630.5 62 26407.0 63 26452.0 64 26497.0 65 22825.0 66 15750.5 67 12348.0 68 10793.5 69 9239.0 70 7642.0 71 6045.0 72 5273.0 73 3789.0 74 3077.0 75 2448.0 76 1819.0 77 1513.5 78 981.5 79 755.0 80 588.0 81 421.0 82 308.0 83 195.0 84 159.5 85 89.0 86 54.0 87 37.0 88 20.0 89 15.5 90 11.0 91 7.5 92 3.5 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 3.1110295330033565E-4 17 6.222059066006713E-4 18 3.1110295330033565E-4 19 3.1110295330033565E-4 20 0.0018666177198020139 21 0.002488823626402685 22 0.0 23 0.0015555147665016783 24 3.1110295330033565E-4 25 3.1110295330033565E-4 26 0.01804397129141947 27 0.005599853159406042 28 0.02302161854422484 29 0.01368852994521477 30 0.03919897211584229 31 0.03671014848943961 32 0.013999632898515106 33 0.006844264972607385 34 0.011821912225412757 35 0.0130663240386141 36 0.032976913049835584 37 0.03639904553613928 38 0.05008757548135405 39 0.03328801600313592 40 0.041998898695545316 41 0.04355441346204699 42 0.0662649290529715 43 0.06377610542656881 44 0.043865516415347326 45 0.040132280975743305 46 0.04604323708844968 47 0.08524220920429197 48 0.08026456195148661 49 0.09706412142970473 50 0.09955294505610741 51 0.07777573832508392 52 0.06813154677277351 53 0.09488640075660239 54 0.10173066572920976 55 0.12941882857293965 56 0.14435177033135577 57 0.13999632898515105 58 0.1446628732846561 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 321437.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.554174516697465 #Duplication Level Percentage of deduplicated Percentage of total 1 70.56426648387806 22.26597179275034 2 11.369438380010708 7.175064855993921 3 4.87307592722382 4.612976647222132 4 3.009931800314119 3.799036532418764 5 1.8733178604189862 2.9555499346453495 6 1.3824958710037223 2.6174109589359076 7 1.0836048307046262 2.393457915463313 8 0.8306970598344583 2.0969567997219176 9 0.650762678584083 1.8480851216096088 >10 3.7896179946223865 21.371106203063515 >50 0.32388188611384744 7.08353773267877 >100 0.22891651827182424 13.817267238722605 >500 0.011995625411623979 2.6432758694326086 >1k 0.006997448156780653 3.6961702431994405 >5k 9.996354509686648E-4 1.624132154141826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 5149 1.6018691065434285 No Hit GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 2357 0.7332696609288912 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 2319 0.7214477487034785 No Hit GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1694 0.5270084028907687 No Hit GTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1535 0.4775430333160153 No Hit GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1414 0.4398995759666746 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1345 0.4184334721889515 No Hit GTGGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 1054 0.3279025127785538 No Hit GTGGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 924 0.28745912884951014 No Hit GTGGTAGGGAGCTGCATTGAGAAGAGAGACAAGAGATCCAGTGAGCCTCTCTACCCCA 910 0.28310368750330545 No Hit GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 804 0.25012677445346987 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 770 0.2395492740412585 No Hit GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 719 0.22368302342294136 No Hit GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 711 0.22119419979653868 No Hit GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 673 0.20937228757112591 No Hit GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 668 0.20781677280462424 No Hit GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 617 0.1919505221863071 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 559 0.17390655089488766 No Hit GTGGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 515 0.16021802094967286 No Hit GTGGTAGGGCATATTATTACAGTATTCAGCATGCCCTATGATATATAGAAATGCACAC 510 0.1586625061831712 No Hit GTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 472 0.14684059395775845 No Hit GTGGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 460 0.14310735851815443 No Hit GTGGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 446 0.1387519171719497 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG 446 0.1387519171719497 No Hit GTGGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 437 0.1359519905922467 No Hit GTGGTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG 427 0.13284096105924334 No Hit GTGGTAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT 418 0.1300410344795403 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA 402 0.12506338722673493 No Hit GTGGTAGGGGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATAT 399 0.12413007836683393 No Hit GTGGTAGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 393 0.12226346064703193 No Hit GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC 390 0.12133015178713091 No Hit GTGGTAGGGACTCTATGTGCTTATAATATAGCCTTGCTGCTACTCGTGAACCTACGCA 389 0.12101904883383059 No Hit GTGGTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 385 0.11977463702062925 No Hit GTGGTAGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAGA 378 0.11759691634752689 No Hit GTGGTAGGGCCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 368 0.11448588681452353 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 368 0.11448588681452353 No Hit GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 363 0.11293037204802185 No Hit GTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTCCG 362 0.1126192690947215 No Hit GTGGTAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG 360 0.11199706318812085 No Hit GTGGTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 351 0.10919713660841783 No Hit GTGGTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 349 0.10857493070181715 No Hit GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA 339 0.1054639011688138 No Hit GTGGTAGGGGAAGTGTTCCTTTGGCAGACGCTGAGGCCGGGGGAGAGACTCTGAACTT 337 0.10484169526221312 No Hit GTGGTAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG 336 0.1045305923089128 No Hit GTGGTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT 334 0.10390838640231212 No Hit GTGGTAGGGGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTCCGACTCAGACGCC 331 0.1029750775424111 No Hit GTGGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC 330 0.10266397458911078 No Hit GTGGTAGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT 329 0.10235287163581044 No Hit GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT 328 0.1020417686825101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.006533162019307049 0.0 0.0 0.0 0.0 11 0.023954927404125848 0.0 0.0 0.0 0.0 12 0.02457713331072652 0.0 0.0 0.0 0.0 13 0.02457713331072652 0.0 0.0 0.0 0.0 14 0.039510075069142635 0.0 0.0 0.0 0.0 15 0.04044338392904364 0.0 0.0 0.0 0.0 16 0.04106558983564431 0.0 0.0 0.0 0.0 17 0.041376692788944645 0.0 0.0 0.0 0.0 18 0.04168779574224498 0.0 0.0 0.0 0.0 19 0.04168779574224498 0.0 0.0 0.0 0.0 20 0.05973176703366445 0.0 0.0 0.0 0.0 21 0.06066507589356546 0.0 0.0 0.0 0.0 22 0.0619094877067668 0.0 0.0 0.0 0.0 23 0.06937595858597485 0.0 0.0 0.0 0.0 24 0.07808684127838425 0.0 0.0 0.0 0.0 25 0.07964235604488594 0.0 0.0 0.0 0.0 26 0.08150897376468795 0.0 0.0 0.0 0.0 27 0.08244228262458896 0.0 0.0 0.0 0.0 28 0.14248515261155373 0.0 0.0 0.0 0.0 29 0.14341846147145476 0.0 0.0 0.0 0.0 30 0.17203993317508562 0.0 0.0 0.0 0.0 31 0.174217653848188 0.0 0.0 0.0 0.0 32 0.17452875680148833 0.0 0.0 0.0 0.0 33 0.17670647747459067 0.0 0.0 0.0 0.0 34 0.17888419814769302 0.0 0.0 0.0 0.0 35 0.2398603769945588 0.0 0.0 0.0 0.0 36 0.24203809766766116 0.0 0.0 0.0 0.0 37 0.24483802424736417 0.0 0.0 0.0 0.0 38 0.24857125968696822 0.0 0.0 0.0 0.0 39 0.25012677445346987 0.0 0.0 0.0 0.0 40 0.25541552465957557 0.0 0.0 0.0 0.0 41 0.25821545123927864 0.0 0.0 0.0 0.0 42 0.26288199553878366 0.0 0.0 0.0 0.0 43 0.26443751030528534 0.0 0.0 0.0 0.0 44 0.2675485398382887 0.0 0.0 0.0 0.0 45 0.27065956937129204 0.0 0.0 0.0 0.0 46 0.2740817018575957 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGTAT 15 0.007193675 52.039715 52 CCACGTA 15 0.007193675 52.039715 51 TCAGTCT 25 1.6450575E-5 52.039715 51 CGTTCGA 20 3.430967E-4 52.03971 52 TCGATAC 20 3.430967E-4 52.03971 52 ATCGATA 20 3.430967E-4 52.03971 51 AACCTAC 35 3.8531653E-8 52.031605 49 GTCAAAC 15 0.0072025405 52.02351 48 CGAATCT 30 7.952167E-7 52.02351 46 TCCCGAG 15 0.0072069764 52.01541 45 CTACAAT 20 3.438889E-4 52.01541 45 TACTAAG 15 0.0072069764 52.01541 45 ACGTATA 20 3.438889E-4 52.01541 40 CCAAATA 15 0.0072069764 52.01541 43 GTAACAC 20 3.438889E-4 52.01541 37 TCTATAC 25 1.6496128E-5 52.01541 39 TATCCGG 20 3.438889E-4 52.01541 44 CGTATAG 20 3.4415332E-4 52.007317 41 AGATAGA 15 0.0072114146 52.007317 42 GCACGAC 20 3.4415332E-4 52.007317 42 >>END_MODULE