FastQCFastQC Report
Tue 31 May 2016
SRR212710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319535
Sequences flagged as poor quality0
Sequence length58
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT52561.644890231117092No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA22550.705712989187413No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC22160.6935077534542382No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT15030.4703710078708122No Hit
GTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC14360.4494030387907428No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG14330.44846417450357556No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG11640.3642793434209085No Hit
GTGGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT9800.30669566714131474No Hit
GTGGTAGGGAGCTGCATTGAGAAGAGAGACAAGAGATCCAGTGAGCCTCTCTACCCCA9160.28666656234841253No Hit
GTGGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7910.24754721704977548No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC7820.24473062418827357No Hit
GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7560.23659380036615707No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6710.20999264556308384No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA6290.19684854554274178No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT6100.19090240505734896No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.1855821740967343No Hit
GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5890.18433035504717793No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5870.18370444552239973No Hit
GTGGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5420.16962148121489037No Hit
GTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5010.15679033595693742No Hit
GTGGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA5000.15647738119454832No Hit
GTGGTAGGGCATATTATTACAGTATTCAGCATGCCCTATGATATATAGAAATGCACAC4820.1508441954715446No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG4470.13989077878792622No Hit
GTGGTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG4390.13738714068881344No Hit
GTGGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4360.13644827640164614No Hit
GTGGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA4310.13488350258970067No Hit
GTGGTAGGGACTCTATGTGCTTATAATATAGCCTTGCTGCTACTCGTGAACCTACGCA4010.12549485971802776No Hit
GTGGTAGGGGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATAT3940.12330417638130409No Hit
GTGGTAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT3910.12236531209413679No Hit
GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3740.11704508113352215No Hit
GTGGTAGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3660.11454144303440937No Hit
GTGGTAGGGGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTCCGACTCAGACGCC3630.1136025787472421No Hit
GTGGTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT3610.11297666922246388No Hit
GTGGTAGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAGA3600.11266371446007481No Hit
GTGGTAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3560.11141189541051841No Hit
GTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTCCG3500.10953416683618382No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC3490.10922121207379473No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3490.10922121207379473No Hit
GTGGTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3460.10828234778662744No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA3410.10671757397468197No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT3370.10546575492512557No Hit
GTGGTAGGGCCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3300.10327507158840189No Hit
GTGGTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3300.10327507158840189No Hit
GTGGTAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG3250.10171029777645642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGG203.4070204E-452.11348323
CTAACTC150.007153444552.11348327
TGCTATT203.4070204E-452.11348323
TGCTACA150.007153444552.11348324
TCACGTC150.007153444552.11348324
ATAGCGG251.6312953E-552.1134823
AAGTTAC251.6312953E-552.1134823
CATACTT251.6312953E-552.1134823
CATAGCG251.6328118E-552.10530522
CGTAAAC150.00715788652.10530521
TCATACT203.4096616E-452.10530522
ATGCAAT203.4096616E-452.10530522
TCGTTCT251.6434598E-552.04812650
TTTCGAA251.6434598E-552.04812651
CCACGTA307.9260826E-752.04812651
GCACGGT150.00721134752.00736647
CATCAGT353.8671715E-852.00736647
GAACCTA459.276846E-1152.0073648
AAATCTA307.9776873E-751.99921841
ATGATAT503.6379788E-1251.99921838
Produced by FastQC (version 0.11.5)