FastQCFastQC Report
Tue 31 May 2016
SRR212709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321463
Sequences flagged as poor quality0
Sequence length58
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT46891.4586437630458249No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC22140.6887262297682781No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA20280.6308657606007535No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT17150.5334984119478757No Hit
GTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC13100.4075119065024591No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG12540.390091550193957No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10930.34000802580701356No Hit
GTGGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT9560.2973903684094282No Hit
GTGGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC8610.26783797824321925No Hit
GTGGTAGGGAGCTGCATTGAGAAGAGAGACAAGAGATCCAGTGAGCCTCTCTACCCCA8290.25788348892407525No Hit
GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT6940.21588798710893634No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6440.2003340975477738No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC6050.18820206369006698No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.1791808077445927No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5710.17762541878847643No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA5470.1701595517991184No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT4970.15460566223795585No Hit
GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4880.15180596211694658No Hit
GTGGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4780.1486951842047141No Hit
GTGGTAGGGCATATTATTACAGTATTCAGCATGCCCTATGATATATAGAAATGCACAC4570.1421625505890258No Hit
GTGGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA4510.1402960838416863No Hit
GTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4410.13718530592945377No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG3980.12380896090685396No Hit
GTGGTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG3970.12349788311563073No Hit
GTGGTAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT3830.11914279403850521No Hit
GTGGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3680.11447662717015644No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA3650.11354339379648669No Hit
GTGGTAGGGACTCTATGTGCTTATAATATAGCCTTGCTGCTACTCGTGAACCTACGCA3630.11292123821404018No Hit
GTGGTAGGGGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATAT3620.11261016042281692No Hit
GTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTCCG3600.11198800484037044No Hit
GTGGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA3590.11167692704914718No Hit
GTGGTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT3480.10825507134569143No Hit
GTGGTAGGGCCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3470.10794399355446817No Hit
GTGGTAGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3460.10763291576324492No Hit
GTGGTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3360.10452213785101241No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3310.10296674889489615No Hit
GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3270.10172243773000314No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3260.10141135993877988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAATC150.007023002552.35658310
GGCATAT600.052.3565838
GATGTAA203.428938E-452.04590639
GTATACT150.007190273652.04590639
GTACGTA150.007190273652.04590639
ACACTCA459.276846E-1152.02966327
ACGCGAT251.6469307E-552.0296624
GCAAGTA150.007199158452.0296624
AGTCAAT150.007199158452.0296626
CAAGTAC150.007199158452.0296625
CGCGAAC401.879016E-952.0296625
TATGCGT251.6469307E-552.0296626
TCAATAA150.007199158452.0296627
GTCAATA150.007199158452.0296627
TATACTG251.6469307E-552.0296626
TATGTTA150.007199158452.0296624
AGTACAC150.007199158452.0296627
AATAGTT251.651502E-552.00530644
CCTATTA353.8684448E-852.00530652
TAGTGCA503.6379788E-1252.00530643
Produced by FastQC (version 0.11.5)