##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212709_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 321463 Sequences flagged as poor quality 0 Sequence length 58 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.56439465817217 38.0 36.0 38.0 33.0 38.0 2 34.97232029813696 38.0 34.0 38.0 28.0 38.0 3 36.183520342932155 38.0 36.0 38.0 33.0 38.0 4 36.125451451644516 38.0 35.0 38.0 32.0 38.0 5 32.74707198029011 35.0 31.0 37.0 23.0 38.0 6 36.58329263398898 38.0 36.0 38.0 33.0 39.0 7 36.732488653437564 38.0 36.0 38.0 33.0 39.0 8 36.70294248482718 38.0 36.0 38.0 33.0 39.0 9 36.61383114075337 38.0 36.0 38.0 33.0 39.0 10 34.48682741093065 38.0 33.0 38.0 27.0 39.0 11 35.325297157060064 38.0 35.0 39.0 29.0 39.0 12 35.29991632007416 38.0 35.0 39.0 29.0 39.0 13 35.176623748300734 38.0 34.0 39.0 29.0 39.0 14 34.984915837903586 38.0 33.0 39.0 28.0 39.0 15 35.24199363534839 38.0 34.0 39.0 29.0 39.0 16 34.796589343097025 38.0 33.0 38.0 28.0 39.0 17 34.45949922697169 37.0 33.0 38.0 27.0 39.0 18 34.48151731303447 36.0 33.0 38.0 27.0 39.0 19 34.06760964714446 36.0 33.0 38.0 27.0 39.0 20 34.124406230266004 36.0 33.0 38.0 27.0 39.0 21 33.84688128960409 36.0 33.0 38.0 26.0 39.0 22 33.77082276965 36.0 33.0 38.0 26.0 39.0 23 33.50596180586879 36.0 32.0 38.0 25.0 39.0 24 33.41716776114203 36.0 32.0 38.0 25.0 39.0 25 33.398580863116436 36.0 32.0 38.0 25.0 39.0 26 32.72444729253444 36.0 32.0 38.0 23.0 39.0 27 32.62313547748886 36.0 31.0 38.0 23.0 39.0 28 32.13461580337395 35.0 30.0 38.0 22.0 39.0 29 32.034066128916855 35.0 30.0 38.0 21.0 39.0 30 31.64510068032713 35.0 30.0 38.0 18.0 39.0 31 30.819559327200952 35.0 29.0 38.0 14.0 39.0 32 30.442641921465302 35.0 28.0 38.0 12.0 39.0 33 30.45317812625403 35.0 28.0 38.0 13.0 39.0 34 29.866830708355238 34.0 27.0 38.0 10.0 39.0 35 29.299070188482034 34.0 26.0 38.0 7.0 39.0 36 30.501945791584102 35.0 29.0 38.0 2.0 39.0 37 30.34081371728628 35.0 29.0 38.0 2.0 39.0 38 30.19092088358536 35.0 28.0 38.0 2.0 39.0 39 29.776282184885975 35.0 28.0 38.0 2.0 39.0 40 29.977160668568388 35.0 28.0 38.0 2.0 39.0 41 30.194902679313017 35.0 29.0 38.0 2.0 39.0 42 29.94949029903908 35.0 28.0 38.0 2.0 39.0 43 29.77074811098011 35.0 28.0 38.0 2.0 39.0 44 29.41699044680103 35.0 27.0 38.0 2.0 39.0 45 29.268802319396013 35.0 27.0 38.0 2.0 39.0 46 28.68570877519341 35.0 26.0 38.0 2.0 39.0 47 28.66753561063015 35.0 26.0 38.0 2.0 39.0 48 28.699094452549748 35.0 26.0 38.0 2.0 39.0 49 28.488690766900078 34.0 26.0 38.0 2.0 39.0 50 28.03781772707901 34.0 24.0 38.0 2.0 39.0 51 27.90845291682091 35.0 24.0 38.0 2.0 39.0 52 27.71326404593997 34.0 24.0 38.0 2.0 39.0 53 27.50562273107636 34.0 23.0 38.0 2.0 39.0 54 27.245667464062738 34.0 23.0 38.0 2.0 39.0 55 27.038632750892017 34.0 22.0 38.0 2.0 39.0 56 26.662272174402652 34.0 16.0 38.0 2.0 39.0 57 26.327708009942047 34.0 15.0 38.0 2.0 39.0 58 25.928663640916685 33.0 2.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -2.6685918757968494 1 2 -6.856168976185508 1 3 -4.7621066946542 1 4 -5.294196928867002 1 5 -7.467818641618045 1 6 -5.3409851854375106 1 7 -4.839539128843349 1 8 -4.535202404306268 1 9 -3.918978775823433 1 10 -5.195153050793259 1 11 -4.75861507770076 1 12 -4.698903638898543 1 13 -4.329819829792392 1 14 -5.2200229781000544 1 15 -4.323213316795787 1 16 -4.698094183050671 1 17 -3.7925032080685668 1 18 -4.73293178078216 1 19 -4.357831132361234 1 20 -4.64872318622119 1 21 -4.446769719148492 1 22 -4.797793678528606 1 23 -5.891840297349283 1 24 -4.913487010997329 1 25 -5.504570677067328 1 26 -5.472891575134064 1 27 -5.543722716689679 1 28 -4.90132290137818 1 29 -4.808001200160209 1 30 -5.342956957772117 1 31 -4.231290225271838 1 32 -4.27059620459454 1 33 -3.8717937878686826 1 34 -3.909126719728917 1 35 -4.814450906100664 1 36 -3.4099884726529694 1 37 -3.3347753668648927 1 38 -3.3348814720717463 1 39 -2.9209410665418645 1 40 -3.5732432108301886 1 41 -2.543871304651372 1 42 -2.5590210541600165 1 43 -2.796028522666578 1 44 -3.136446048745807 1 45 -2.8888185359930283 1 46 -1.818109845831895 1 47 -2.9639590895992676 1 48 -3.271328452735986 1 49 -2.332551136447435 1 50 -3.376978616333279 1 51 -4.710449325178708 1 52 -4.8939941218918115 1 53 -3.901555050345735 1 54 -4.086219462569048 1 55 -4.512967258057806 1 56 -4.573060654280052 1 57 -4.498260846139548 1 58 -4.984687522311649 2 1 -0.7201543757968523 2 2 -0.6797290252051127 2 3 -0.9136998319090992 2 4 -2.0050425171022965 2 5 -0.7259987886768684 2 6 -0.8937119011237868 2 7 -0.9422045700198183 2 8 -1.3426166199925404 2 9 -0.9008415209214746 2 10 -0.49640917824424236 2 11 -1.0470035580929178 2 12 -0.8302761879181517 2 13 -0.7033798788120009 2 14 -0.821340380060839 2 15 -1.0885319442467676 2 16 -1.0939275163840065 2 17 -0.7392556590489576 2 18 -0.718470996468433 2 19 -1.3553188774592755 2 20 -0.9136741666133474 2 21 -0.853295454442609 2 22 -1.0923402471560593 2 23 -0.7955167679375208 2 24 -1.0412443639385032 2 25 -1.3875057260869355 2 26 -0.4329405947419076 2 27 -0.7985021284543841 2 28 -1.2415496170644538 2 29 -0.8621678668268764 2 30 -1.2164250950270201 2 31 -1.2330059115463463 2 32 -1.3210251261631676 2 33 -0.9872962388490762 2 34 -1.6034281903171532 2 35 -1.2636849747281147 2 36 -0.8254296491235564 2 37 -1.0053329649041096 2 38 -0.8491890701109632 2 39 -0.6195930273261787 2 40 -0.7599466422027383 2 41 -0.419729147788626 2 42 -0.89079188749335 2 43 -0.8590493559999111 2 44 -0.7355269310987467 2 45 -0.7854790752087162 2 46 -0.6305485713220911 2 47 -0.4516429131286799 2 48 -1.293417913520301 2 49 -1.234082999192534 2 50 -0.7941354790783777 2 51 -0.9136662369434134 2 52 -0.6084855434604393 2 53 -0.6366347072084793 2 54 -1.3562255900200277 2 55 -1.3266314737440794 2 56 -0.7570373699663264 2 57 -0.7265083951591542 2 58 -1.5027022281940035 3 1 -0.24172300324782725 3 2 -0.9267878487345271 3 3 -0.22742532210518362 3 4 -0.19327781121994292 3 5 -1.0436458475003967 3 6 -0.0505746462218255 3 7 0.014658175078217539 3 8 0.026010830987850397 3 9 -0.21064544249009742 3 10 0.048688860971445536 3 11 -0.25876826397527 3 12 -0.5479232467416821 3 13 -0.8327916435178793 3 14 -0.13898743888437082 3 15 -0.36696331679578975 3 16 -0.3684373203055742 3 17 -0.17062820806856394 3 18 -0.26749060431156835 3 19 -0.3082600539298639 3 20 -0.4705369117113847 3 21 -1.4297660426779046 3 22 -0.4844971099011559 3 23 -0.9602226502904614 3 24 -1.2373227953110515 3 25 -0.5129959221653664 3 26 -0.385881771212496 3 27 0.08385081272208339 3 28 -0.2258633425546499 3 29 0.0201850743495946 3 30 0.12867294418866848 3 31 1.0611117355124762 3 32 0.733876834621146 3 33 0.6205468984058271 3 34 0.7534345547808883 3 35 1.002981691938551 3 36 1.2608448606803648 3 37 1.226039584115501 3 38 1.0959089691047232 3 39 1.9255050118895092 3 40 1.9224062989737334 3 41 1.0979179110349016 3 42 1.2896002693693944 3 43 1.140950644000089 3 44 1.7232965983130164 3 45 1.1204032777324606 3 46 1.1616082914230077 3 47 1.336592380988968 3 48 1.235993851185583 3 49 1.0874856282584489 3 50 0.896060599352996 3 51 1.6431965081546274 3 52 1.607200731049364 3 53 1.5006201947523046 3 54 1.3653430374309536 3 55 1.081211663510821 3 56 0.6194332182689664 3 57 0.25388376170359095 3 58 0.3639644384726637 4 1 -0.5719472044821075 4 2 -0.8523405399305304 4 3 -0.5640240253314914 4 4 -0.7496874667156064 4 5 -1.0422397073550975 4 6 -0.27615077308691127 4 7 -0.43275999537721077 4 8 -0.8557327827923231 4 9 -0.5559309670584938 4 10 0.15902399103941178 4 11 0.238614752976531 4 12 0.11185333321301272 4 13 -0.0281826285971718 4 14 0.06412169321468753 4 15 -0.031505149465111515 4 16 -0.4578053384291536 4 17 -1.0877596821721554 4 18 -0.6475234142482975 4 19 -1.1697708733970913 4 20 -0.8232749790498772 4 21 -1.8783247490289696 4 22 -1.4055501526321947 4 23 -1.3029458750385245 4 24 -1.3154416922722305 4 25 -1.388005687027487 4 26 -1.309793965572311 4 27 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0.35885511355256483 5 50 0.9667713836667211 5 51 0.6333006748212924 5 52 0.3599121281081885 5 53 0.3679761996542652 5 54 0.5817492874309522 5 55 0.28390774194219404 5 56 0.8566268457199477 5 57 0.1579891538604521 5 58 0.12859372768835087 6 1 -0.2481237244558514 6 2 -0.5341802309747763 6 3 -0.36639309312162993 6 4 -0.4270393618171937 6 5 -0.9728773102004205 6 6 -0.001036670111837168 6 7 -0.24605852539507111 6 8 -0.21162721273538665 6 9 -0.264285289233392 6 10 0.30715178637149165 6 11 -0.2387096658233645 6 12 -0.25637490326635515 6 13 -0.03752457117170138 6 14 -0.20592455855982195 6 15 -0.266580175033333 6 16 -0.6724715776866859 6 17 -0.19055157971607883 6 18 -0.5698570681384822 6 19 -0.7340883162692791 6 20 -0.3046139908188934 6 21 -0.6141248436695648 6 22 -0.9949320884902946 6 23 -0.8144935540542662 6 24 -0.9100746546754905 6 25 -0.32548183607116243 6 26 -0.31173257130264886 6 27 -0.06922559025289843 6 28 -0.32741844735519265 6 29 0.1079470757018619 6 30 0.35693528360719284 6 31 0.056784487371839276 6 32 0.4661734314207848 6 33 0.7077679937405144 6 34 0.26035364425575835 6 35 0.4797001954319029 6 36 0.35509773424358215 6 37 0.10951941282859323 6 38 0.6947820815680998 6 39 1.0322437993585183 6 40 0.7334497968326481 6 41 0.9339783122068646 6 42 0.9659130933495632 6 43 0.9026364677548777 6 44 1.1081061543193265 6 45 0.9511898452330243 6 46 1.303956725049332 6 47 0.6866036498643346 6 48 0.5530549284900665 6 49 0.9565412965027562 6 50 1.5803968625939255 6 51 0.4700164315262718 6 52 0.40476665397025613 6 53 0.8042938481216986 6 54 0.7691744550554738 6 55 1.0825188530533048 6 56 1.191576558363625 6 57 1.3945193262742457 6 58 1.1435452363090413 7 1 -0.012691689229683334 7 2 -0.34462513290192476 7 3 -0.05361788868405171 7 4 -0.08827342140431682 7 5 -0.20277666400610173 7 6 0.178954180234129 7 7 0.2356707592163545 7 8 0.2024375210668694 7 9 0.21348391074373296 7 10 0.7520251208485291 7 11 0.36253697453804534 7 12 0.43169337602682845 7 13 0.51754490901358 7 14 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0.3416037068668061 119 6 0.3505394357746141 119 7 0.05226958327786235 119 8 0.06375592902706728 119 9 0.16056667872202013 119 10 0.5645533886006788 119 11 0.35999287684469294 119 12 -0.33905515405005815 119 13 -0.030340663125727474 119 14 0.1272781582635787 119 15 -0.3336299834624512 119 16 -0.21892751638400654 119 17 -0.3479009353412934 119 18 -0.7196742050245817 119 19 -0.22194098084608527 119 20 -0.1468292468272523 119 21 -0.7524041888454605 119 22 -0.283115648225575 119 23 -0.19297666098565003 119 24 -0.27096807160339154 119 25 -0.647799843733992 119 26 -0.38387642361891494 119 27 -0.556033322750288 119 28 -0.20790434077211728 119 29 0.02343819377918166 119 30 -0.6578243820145424 119 31 0.04333098684937298 119 32 -0.25591817429150865 119 33 -0.4451839393838348 119 34 -0.6425547500319482 119 35 -0.8923391636764215 119 36 -0.5633975635620594 119 37 -1.3047090789861038 119 38 -1.254625790253563 119 39 -0.8404486422994388 119 40 -1.4638682108301886 119 41 -1.105424334954403 119 42 -1.010488857190321 119 43 -0.8772311741817305 119 44 -0.8405180184427756 119 45 -0.7441689147809072 119 46 -0.2144166640137115 119 47 -0.6651901502053299 119 48 -0.23321292243295488 119 49 -0.46844128796258744 119 50 -0.4906149799696422 119 51 -0.9355913706332544 119 52 -0.8312573794675693 119 53 -0.6835152776184614 119 54 -0.6361721140842 119 55 -0.8316225610881105 119 56 -0.8711193664012633 119 57 -0.7686206946243956 119 58 -0.5538163101904381 120 1 -1.2217672790226572 120 2 -1.7193242181209882 120 3 -1.7469857269122642 120 4 -2.0124428966089383 120 5 -1.2730101738761093 120 6 -1.0811565564052543 120 7 -1.2364645320691565 120 8 -1.3703838559191723 120 9 -1.2980110338879527 120 10 -0.7458082927287393 120 11 -0.2760041906039845 120 12 -0.5739036388985426 120 13 -0.7494266846311035 120 14 -0.5413435426161826 120 15 -0.7274471877635307 120 16 -0.8331748282119591 120 17 -0.31820885322985504 120 18 -0.34381887755635177 120 19 -1.0710367775225222 120 20 -0.36041673460828605 120 21 -1.5891588320517158 120 22 -0.7368057753027983 120 23 -0.780953200575091 120 24 -1.4603620109973292 120 25 -0.8843589835189434 120 26 -0.2007141557792238 120 27 -0.5346501360445188 120 28 -0.4177039497652757 120 29 -0.13663023241827332 120 30 -0.3384206674495367 120 31 -0.02766119301377401 120 32 -0.6208986239493797 120 33 0.17407314761518933 120 34 -0.4549936552127889 120 35 -0.36863437384259967 120 36 0.6404147531534825 120 37 0.2985411653931713 120 38 0.4224269956701896 120 39 1.0619218366839434 120 40 0.9030672730407794 120 41 0.7393948243808879 120 42 0.9538317684206277 120 43 1.2178323644301976 120 44 1.197727338350969 120 45 1.2797802543295518 120 46 1.466668379974557 120 47 1.4679965555620242 120 48 1.248580821457562 120 49 1.3806545087138566 120 50 1.977860093344141 120 51 1.6905083361116162 120 52 1.7005119264952846 120 53 1.5840800302994253 120 54 1.6927623922696604 120 55 1.9109238709744538 120 56 2.2160018457199477 120 57 2.108089960312064 120 58 2.213901187365771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 954.0 3 71.0 4 200.0 5 273.0 6 597.0 7 1019.0 8 568.0 9 899.0 10 1138.0 11 1211.0 12 1619.0 13 1980.0 14 2163.0 15 2684.0 16 3416.0 17 3206.0 18 3677.0 19 3780.0 20 2777.0 21 3024.0 22 3546.0 23 4191.0 24 4116.0 25 4573.0 26 5574.0 27 6662.0 28 8406.0 29 10590.0 30 12857.0 31 15905.0 32 19596.0 33 24187.0 34 29178.0 35 35086.0 36 39554.0 37 40512.0 38 18500.0 39 3174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 57.7374137839101 28.142750948024126 4.873581100366663 9.246254167699117 11 32.2396989288816 27.66386617401238 16.557666387551244 23.538768509554775 12 40.24195630601344 13.096997166081323 26.86623343899609 19.794813088909144 13 21.516939741121064 14.536043028280082 31.775974217872665 32.171043012726194 14 21.05747784348432 23.00171403862964 36.04613905799423 19.894669059891807 15 30.551260953826727 13.58663360946672 34.16380734330234 21.698298093404215 16 26.350155383356714 15.981932601885754 34.448754600062834 23.219157414694696 17 35.22400878795329 15.818315911983543 22.450357710775513 26.50731758928766 18 33.82286608412166 16.932897409655233 25.569350127386354 23.674886378836757 19 28.050817667974233 19.727931363796145 29.936260160578353 22.28499080765127 20 28.95601318982144 25.672556461146023 23.04610215890002 22.32532819013252 21 28.623067806048013 19.24245864972734 31.53570728642044 20.598766257804215 22 31.234774925566306 18.411645568045 24.218251733675142 26.135327772713556 23 33.491050264105866 24.50802894276773 22.887619687552494 19.11330110557391 24 32.559890251755256 24.74406074727107 21.67838911476593 21.017659886207742 25 29.07462946600794 19.74719066167229 20.229413741179986 30.948766131139788 26 32.20172837124194 17.607976523527817 24.218980964265597 25.97131414096464 27 23.933546795117138 19.119765752141618 22.602055580967672 34.344631871773565 28 26.453197064663303 27.176450084960447 21.588355750857385 24.78199709951887 29 34.27604100155422 19.360806824871286 22.781652084806314 23.581500088768177 30 26.158471988533684 23.7845080077273 25.99956378139216 24.057456222346858 31 35.75730801299202 17.95487636422465 24.998755553343205 21.289060069440126 32 34.17440326554994 16.940662568136 29.98357269084302 18.90136147547104 33 36.244446380346716 16.23523701666397 26.690353813796623 20.829962789192688 34 28.947605875709026 19.335949046488548 31.705617145470782 20.010827932331647 35 25.8485387832321 28.491237677984664 29.629468286368617 16.030755252414615 36 28.34886602045708 27.92957685100449 25.545200430806393 18.17635669773204 37 27.9938866287123 24.660791936824346 30.10648596321394 17.238835471249413 38 28.219487671173177 25.092221056596493 27.837840361848965 18.850450910381365 39 28.396316092939177 22.840223777095574 27.301740661669598 21.461719468295648 40 26.667040250550254 22.34965583584724 29.331627300264934 21.651676613337568 41 26.932752179327522 21.66376089663761 29.805105853051057 21.59838107098381 42 23.60155427831296 22.59401328858141 30.12192615932598 23.682506273779648 43 26.249381109114744 18.827672752297293 29.857476046198062 25.0654700923899 44 25.863057940382443 18.400576099358435 30.36835443827195 25.368011521987167 45 25.94179521831464 20.02821181907069 30.98442433565214 23.045568626962528 46 24.961303081738482 22.809536415590888 30.90490057149976 21.324259931170875 47 24.80173686604078 21.75941788822507 31.889994330889177 21.548850914844973 48 27.752617907044836 20.12035208591594 30.368033190264686 21.75899681677454 49 25.21860485400903 20.986377498730583 31.388759956761874 22.406257690498517 50 22.774849538335015 20.192703076520505 32.70750003115148 24.324947353992997 51 19.774365109779694 21.53096482127112 34.6693211947869 24.02534887416228 52 21.9874731233056 20.274843414041317 36.413636221993706 21.324047240659375 53 20.91177872557847 20.24412964168772 36.37643818715983 22.467653445573983 54 20.60304325610957 21.38161584188029 34.63261592911531 23.382724972894835 55 21.300235301450765 20.338460100042074 32.060523896342694 26.30078070216446 56 21.830968345548104 21.893341649773898 28.416653672228286 27.859036332449712 57 24.75773094654028 22.24043937273433 29.53453795106929 23.4672917296561 58 24.131404749952452 25.39793401700554 28.91534333793757 21.555317895104437 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 80.0 10 160.0 11 207.0 12 254.0 13 283.5 14 313.0 15 372.5 16 784.0 17 1136.0 18 1513.0 19 1890.0 20 1852.5 21 1815.0 22 2101.0 23 2766.5 24 3146.0 25 3878.0 26 4610.0 27 5023.0 28 5851.5 29 6267.0 30 6254.0 31 6241.0 32 6009.0 33 5777.0 34 8000.0 35 7919.0 36 5615.0 37 5089.0 38 4563.0 39 4435.5 40 4308.0 41 4361.0 42 4639.0 43 4864.0 44 5684.0 45 6504.0 46 7727.5 47 8436.5 48 7922.0 49 7874.5 50 7827.0 51 9139.0 52 10451.0 53 12093.5 54 14877.5 55 16019.0 56 16851.0 57 17683.0 58 20679.0 59 25436.0 60 27197.0 61 27052.5 62 26908.0 63 26526.0 64 26144.0 65 22129.5 66 14929.5 67 11744.0 68 10192.0 69 8640.0 70 7125.5 71 5611.0 72 4848.5 73 3396.0 74 2706.0 75 2131.5 76 1557.0 77 1269.0 78 787.5 79 594.0 80 442.5 81 291.0 82 214.0 83 137.0 84 106.0 85 55.5 86 36.0 87 24.0 88 12.0 89 8.0 90 4.0 91 4.5 92 3.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.8218675244118296 11 0.06532633615688276 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.03546286819945063 18 0.0 19 0.0 20 9.332333736697537E-4 21 6.221555824465024E-4 22 0.011198800484037043 23 3.110777912232512E-4 24 0.0 25 0.010887722692813793 26 0.03732933494679014 27 0.02924131237498561 28 0.04261765739758541 29 0.125053272071747 30 0.16269368480976037 31 0.010887722692813793 32 0.014620656187492805 33 0.015864967352385812 34 0.022397600968074086 35 0.027063767836422857 36 0.0637709472007665 37 0.062215558244650235 38 0.06968142523400826 39 0.18913529706373675 40 0.0777694478058128 41 0.08181345909171507 42 0.08865717049862659 43 0.1007892043563334 44 0.2130882869879271 45 0.09985597098266363 46 0.11758740508238895 47 0.13220806126988177 48 0.12598650544541673 49 0.13998500605046305 50 0.1402960838416863 51 0.15616105119407211 52 0.1720260185464579 53 0.1511838065345001 54 0.15273919549061635 55 0.1860245191515042 56 0.2529062442645032 57 0.19971194196532727 58 0.2314418766700989 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 321463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.56532785479281 #Duplication Level Percentage of deduplicated Percentage of total 1 77.7699826152329 30.76994859433227 2 8.953054557062991 7.084610777040881 3 3.816153339399665 4.5296207405253055 4 2.1698833014487935 3.4340857691384694 5 1.405012034811918 2.779488089863155 6 1.0299368187277553 2.4449872741611567 7 0.7816631009176715 2.1648729801861175 8 0.5760035852334978 1.8231816556239546 9 0.5116445977089588 1.8219047628139675 >10 2.5950880332244144 18.5117054583004 >50 0.21501478245974692 5.963684671040602 >100 0.16400762603681424 12.28038420416852 >500 0.007062529350867599 1.9507408781834708 >1k 0.005493078384008133 4.4407841446217065 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 4689 1.4586437630458249 No Hit GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 2214 0.6887262297682781 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 2028 0.6308657606007535 No Hit GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1715 0.5334984119478757 No Hit GTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1310 0.4075119065024591 No Hit GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1254 0.390091550193957 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1093 0.34000802580701356 No Hit GTGGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 956 0.2973903684094282 No Hit GTGGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 861 0.26783797824321925 No Hit GTGGTAGGGAGCTGCATTGAGAAGAGAGACAAGAGATCCAGTGAGCCTCTCTACCCCA 829 0.25788348892407525 No Hit GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 694 0.21588798710893634 No Hit GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 644 0.2003340975477738 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 605 0.18820206369006698 No Hit GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 576 0.1791808077445927 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 571 0.17762541878847643 No Hit GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 547 0.1701595517991184 No Hit GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 497 0.15460566223795585 No Hit GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 488 0.15180596211694658 No Hit GTGGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 478 0.1486951842047141 No Hit GTGGTAGGGCATATTATTACAGTATTCAGCATGCCCTATGATATATAGAAATGCACAC 457 0.1421625505890258 No Hit GTGGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 451 0.1402960838416863 No Hit GTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 441 0.13718530592945377 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG 398 0.12380896090685396 No Hit GTGGTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG 397 0.12349788311563073 No Hit GTGGTAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT 383 0.11914279403850521 No Hit GTGGTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 368 0.11447662717015644 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA 365 0.11354339379648669 No Hit GTGGTAGGGACTCTATGTGCTTATAATATAGCCTTGCTGCTACTCGTGAACCTACGCA 363 0.11292123821404018 No Hit GTGGTAGGGGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATAT 362 0.11261016042281692 No Hit GTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTCCG 360 0.11198800484037044 No Hit GTGGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 359 0.11167692704914718 No Hit GTGGTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 348 0.10825507134569143 No Hit GTGGTAGGGCCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 347 0.10794399355446817 No Hit GTGGTAGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 346 0.10763291576324492 No Hit GTGGTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 336 0.10452213785101241 No Hit GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA 331 0.10296674889489615 No Hit GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 327 0.10172243773000314 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 326 0.10141135993877988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.009954489319144038 0.0 0.0 0.0 0.0 11 0.029552390166208864 0.0 0.0 0.0 0.0 12 0.03110777912232512 0.0 0.0 0.0 0.0 13 0.03110777912232512 0.0 0.0 0.0 0.0 14 0.04541735751859467 0.0 0.0 0.0 0.0 15 0.046350590892264426 0.0 0.0 0.0 0.0 16 0.04728382426593418 0.0 0.0 0.0 0.0 17 0.047905979848380686 0.0 0.0 0.0 0.0 18 0.049150291013273686 0.0 0.0 0.0 0.0 19 0.05039460217816669 0.0 0.0 0.0 0.0 20 0.06843711406911526 0.0 0.0 0.0 0.0 21 0.06937034744278502 0.0 0.0 0.0 0.0 22 0.07310328093746403 0.0 0.0 0.0 0.0 23 0.07808052559703606 0.0 0.0 0.0 0.0 24 0.08896824828984984 0.0 0.0 0.0 0.0 25 0.08990148166351959 0.0 0.0 0.0 0.0 26 0.09207902620208236 0.0 0.0 0.0 0.0 27 0.09332333736697536 0.0 0.0 0.0 0.0 28 0.13469668359966777 0.0 0.0 0.0 0.0 29 0.13687422813823052 0.0 0.0 0.0 0.0 30 0.16487122934832313 0.0 0.0 0.0 0.0 31 0.1658044627219929 0.0 0.0 0.0 0.0 32 0.16673769609566264 0.0 0.0 0.0 0.0 33 0.16922631842544866 0.0 0.0 0.0 0.0 34 0.17233709633768116 0.0 0.0 0.0 0.0 35 0.2460625328575917 0.0 0.0 0.0 0.0 36 0.2504176219347172 0.0 0.0 0.0 0.0 37 0.2532173220557265 0.0 0.0 0.0 0.0 38 0.2544616332206195 0.0 0.0 0.0 0.0 39 0.25632809996795897 0.0 0.0 0.0 0.0 40 0.25850564450652175 0.0 0.0 0.0 0.0 41 0.2603721112538612 0.0 0.0 0.0 0.0 42 0.26410504474854024 0.0 0.0 0.0 0.0 43 0.266282589287103 0.0 0.0 0.0 0.0 44 0.267526900451996 0.0 0.0 0.0 0.0 45 0.2703266005730053 0.0 0.0 0.0 0.0 46 0.27374845627646105 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAATC 15 0.0070230025 52.356583 10 GGCATAT 60 0.0 52.356583 8 GATGTAA 20 3.428938E-4 52.045906 39 GTATACT 15 0.0071902736 52.045906 39 GTACGTA 15 0.0071902736 52.045906 39 ACACTCA 45 9.276846E-11 52.029663 27 ACGCGAT 25 1.6469307E-5 52.02966 24 GCAAGTA 15 0.0071991584 52.02966 24 AGTCAAT 15 0.0071991584 52.02966 26 CAAGTAC 15 0.0071991584 52.02966 25 CGCGAAC 40 1.879016E-9 52.02966 25 TATGCGT 25 1.6469307E-5 52.02966 26 TCAATAA 15 0.0071991584 52.02966 27 GTCAATA 15 0.0071991584 52.02966 27 TATACTG 25 1.6469307E-5 52.02966 26 TATGTTA 15 0.0071991584 52.02966 24 AGTACAC 15 0.0071991584 52.02966 27 AATAGTT 25 1.651502E-5 52.005306 44 CCTATTA 35 3.8684448E-8 52.005306 52 TAGTGCA 50 3.6379788E-12 52.005306 43 >>END_MODULE