FastQCFastQC Report
Tue 31 May 2016
SRR212708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212708_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318267
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT56261.767698190512997No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC26030.8178667596703397No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC18670.5866143835207547No Hit
GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC13860.435483414868648No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC13100.41160409341841914No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11290.35473360417511085No Hit
GTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA11130.3497063786066416No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11010.34593595943028965No Hit
GTGGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA8680.27272698708945636No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC7820.24570564965893416No Hit
GTGGTAGGGAGCTGCATTGAGAAGAGAGACAAGAGATCCAGTGAGCCTCTCTACCC7540.23690800491411299No Hit
GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA7200.22622515058111584No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC6830.21459969145403074No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6810.2139712882579721No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.1922913779939485No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5850.18380793484715663No Hit
GTGGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA5780.18160852366095134No Hit
GTGGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5630.17689549969051144No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5590.17563869329839413No Hit
GTGGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC5320.16715525015160226No Hit
GTGGTAGGGACTCTATGTGCTTATAATATAGCCTTGCTGCTACTCGTGAACCTACG5320.16715525015160226No Hit
GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4810.15113096865210657No Hit
GTGGTAGGGCATATTATTACAGTATTCAGCATGCCCTATGATATATAGAAATGCAC4580.14390433189743204No Hit
GTGGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4200.13196467117231758No Hit
GTGGTAGGGCACTGCTCACAACAAAAACAGACAAGGCCCACAGGTGCATCAGTTAG4040.12693744560384834No Hit
GTGGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC3930.12348122802552575No Hit
GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3910.12285282482946708No Hit
GTGGTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG3890.12222442163340842No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3770.11845400245705649No Hit
GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGGCCACGCCGAGCGAC3750.11782559926099784No Hit
GTGGTAGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTA3730.11719719606493918No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC3720.11688299446690985No Hit
GTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3680.11562618807479255No Hit
GTGGTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCC3610.11342677688858725No Hit
GTGGTAGGGGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCAT3540.11122736570238197No Hit
GTGGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA3390.10651434173194205No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC3390.10651434173194205No Hit
GTGGTAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT3360.10557173693785406No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC3210.10085871296741415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAC150.00827491850.2284750
ACTTATC204.0862986E-450.22846650
CGAGCCA204.0862986E-450.22846650
CCGATAC150.00828009950.2205249
TATGGCA150.00828009950.2205248
TTACTGG150.00828009950.2205249
ATATGCG150.00828009950.2205248
TATGCGA150.00828009950.2205249
AAACCGG150.00828009950.2205249
AGATTAA950.050.22051649
GAACCTA800.050.22051648
CACTTAT204.0894924E-450.22051649
CGAATCT301.0186359E-650.1887446
GGCATAG252.0380821E-550.1887447
GCCCGAT150.008300845550.1887447
CGCCCAA150.008300845550.1887446
TTCTTAC252.0399908E-550.18079845
CAGATAT150.00830603950.18079844
TGCGAAA150.00830603950.18079844
GTACTTG204.1054926E-450.18079845
Produced by FastQC (version 0.11.5)