FastQCFastQC Report
Tue 31 May 2016
SRR212707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212707_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297778
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT48891.6418271329648262No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC23910.8029471619797298No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC18020.6051488021277596No Hit
GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC17910.60145477503375No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC13690.45973846288174414No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC12230.410708648724889No Hit
GTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA11070.3717534539153329No Hit
GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA10840.3640295790824037No Hit
GTGGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA7760.260596820450134No Hit
GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC7520.2525371249722948No Hit
GTGGTAGGGAGCTGCATTGAGAAGAGAGACAAGAGATCCAGTGAGCCTCTCTACCC7360.2471639946537353No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6950.2333953482124267No Hit
GTGGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA6710.2253356527345875No Hit
GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC6220.20888044113399917No Hit
GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA6130.20585805532980944No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.2014923869459799No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5960.20014910436634004No Hit
GTGGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5330.17899240373701214No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5320.17865658309210217No Hit
GTGGTAGGGACTCTATGTGCTTATAATATAGCCTTGCTGCTACTCGTGAACCTACG5060.16992524632444306No Hit
GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGGCCACGCCGAGCGAC4820.16186555084660384No Hit
GTGGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4410.14809690440529522No Hit
GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4390.1474252631154753No Hit
GTGGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC4240.1423879534418258No Hit
GTGGTAGGGCACTGCTCACAACAAAAACAGACAAGGCCCACAGGTGCATCAGTTAG4040.13567154054362646No Hit
GTGGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA3960.13298497538434673No Hit
GTGGTAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3790.1272760244208773No Hit
GTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3600.12089543216758794No Hit
GTGGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3580.120223790877768No Hit
GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGGGGTCCACGCCGAGCGAC3520.11820886700830821No Hit
GTGGTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG3510.11787304636339824No Hit
GTGGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC3460.1161939431388484No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC3370.11317155733465871No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC3350.11249991604483876No Hit
GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGGGGGCCACGCCGAGCGAC3330.11182827475501883No Hit
GTGGTAGGGCATATTATTACAGTATTCAGCATGCCCTATGATATATAGAAATGCAC3320.11149245411010886No Hit
GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3270.10981335088555903No Hit
GTGGTAGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTA3260.10947753024064907No Hit
GTGGTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCC3250.1091417095957391No Hit
GTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTC3140.10544768250172949No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3140.10544768250172949No Hit
GTGGTAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC3080.10343275863226968No Hit
GTGGTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG3000.10074619347298995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCA150.0082761650.2257648
AACTTAG150.0082761650.2257650
CCAATCG150.0082761650.2257648
ACTTAGC252.0288882E-550.2257647
TTAACTA150.0082761650.2257647
AACCTAC600.050.2257649
ACCAATC150.0082761650.2257647
AACTATT150.0082761650.2257649
ATAGATC204.086955E-450.22575850
TTATCTC402.566594E-950.22575847
AGATTAC204.086955E-450.22575848
TGCACGT204.086955E-450.22575849
TGCATGA150.00828169850.2172646
CGAATCT252.0309208E-550.2172646
TACAATG204.0903696E-450.2172646
TCGTGAA600.050.20876744
TTCTTAC355.1013558E-850.20876745
CATAAGC150.0082872450.20876745
AATTATC402.57387E-950.20876345
CGTTGAC204.0972058E-450.2002743
Produced by FastQC (version 0.11.5)