##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212707_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 297778 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.9358448239964 38.0 37.0 38.0 33.0 39.0 2 35.56827905352309 38.0 34.0 38.0 30.0 38.0 3 36.64634392063886 38.0 36.0 38.0 33.0 38.0 4 36.57470666066667 38.0 36.0 38.0 33.0 38.0 5 33.01348991530603 35.0 32.0 37.0 26.0 38.0 6 36.98951903767236 38.0 37.0 39.0 34.0 39.0 7 37.24737891986648 38.0 38.0 39.0 35.0 39.0 8 37.196777465091444 38.0 38.0 39.0 35.0 39.0 9 37.12581856282197 38.0 38.0 39.0 35.0 39.0 10 35.38974336586316 38.0 35.0 38.0 29.0 39.0 11 35.65054839511314 38.0 35.0 39.0 30.0 39.0 12 35.515320137820794 38.0 35.0 39.0 30.0 39.0 13 35.45119182746878 38.0 35.0 39.0 29.0 39.0 14 35.474350019141774 38.0 35.0 39.0 29.0 39.0 15 35.547760412119096 38.0 35.0 39.0 30.0 39.0 16 35.03737683777848 38.0 34.0 38.0 29.0 39.0 17 34.80669156217048 37.0 33.0 38.0 29.0 39.0 18 34.60745253175184 37.0 33.0 38.0 28.0 39.0 19 34.20491104111116 36.0 33.0 38.0 27.0 39.0 20 34.216547226457294 36.0 33.0 38.0 27.0 39.0 21 33.53607385367623 36.0 33.0 38.0 26.0 39.0 22 33.41175305093056 36.0 32.0 38.0 26.0 39.0 23 33.05446675039795 36.0 32.0 38.0 24.0 39.0 24 32.78993411198947 36.0 31.0 38.0 23.0 39.0 25 32.596021196999104 35.0 31.0 38.0 23.0 39.0 26 31.44957988837322 35.0 30.0 38.0 18.0 39.0 27 31.250115858122495 35.0 30.0 38.0 18.0 39.0 28 30.630352141528252 34.0 29.0 38.0 15.0 39.0 29 30.589418963120178 34.0 29.0 38.0 14.0 39.0 30 29.945969816440435 34.0 29.0 38.0 6.0 39.0 31 28.512472378751955 33.0 25.0 38.0 2.0 39.0 32 28.239604671936814 33.0 25.0 38.0 2.0 39.0 33 28.07661748013621 33.0 25.0 37.0 2.0 39.0 34 28.0158473762333 33.0 25.0 37.0 2.0 39.0 35 27.430300425148936 33.0 23.0 36.0 2.0 39.0 36 27.947947800038957 34.0 25.0 38.0 2.0 39.0 37 27.59384507922009 33.0 24.0 37.0 2.0 39.0 38 27.37057136524525 33.0 23.0 37.0 2.0 39.0 39 27.056518614538348 33.0 23.0 37.0 2.0 39.0 40 26.80951245558772 33.0 22.0 37.0 2.0 39.0 41 26.066149950634365 33.0 16.0 36.0 2.0 39.0 42 25.597068957411224 32.0 13.0 36.0 2.0 39.0 43 25.511924991100752 32.0 12.0 36.0 2.0 39.0 44 25.26378711657678 32.0 9.0 36.0 2.0 39.0 45 24.609007381337776 32.0 2.0 36.0 2.0 39.0 46 23.670422260878908 31.0 2.0 36.0 2.0 39.0 47 23.36173256587122 31.0 2.0 35.0 2.0 39.0 48 23.249709515142154 31.0 2.0 35.0 2.0 39.0 49 22.752271826662817 30.0 2.0 35.0 2.0 39.0 50 22.29825238936389 30.0 2.0 35.0 2.0 39.0 51 21.393786646427877 30.0 2.0 35.0 2.0 39.0 52 21.109104769324798 29.0 2.0 35.0 2.0 39.0 53 20.7413274318452 29.0 2.0 35.0 2.0 39.0 54 20.43278549792127 29.0 2.0 35.0 2.0 38.0 55 19.81360610924917 28.0 2.0 35.0 2.0 38.0 56 11.141857356822868 2.0 2.0 20.0 2.0 24.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6665883218701865 1 2 -0.7503741242171245 1 3 -0.8615178900942482 1 4 -1.0530430631776824 1 5 -1.3285591300854236 1 6 -0.7501168286795803 1 7 -0.9691139918335026 1 8 -0.9452978176080933 1 9 -1.0190421839330455 1 10 -0.6742797449639113 1 11 -0.7577754813060906 1 12 -0.4321081851528561 1 13 -0.6808334446881901 1 14 -0.7363159948504006 1 15 -0.5789156320606139 1 16 -0.2283022990545689 1 17 -0.02122909368236492 1 18 -0.29118269780425976 1 19 -0.13645487168087556 1 20 -0.6610102907876652 1 21 -0.5078258760498002 1 22 -0.9203708457080353 1 23 -0.6623622806960618 1 24 -0.24574343761351258 1 25 -0.5193740217111333 1 26 0.25987669366580235 1 27 0.653135717009075 1 28 0.26211591228706155 1 29 0.7460270320782953 1 30 0.16281894458838053 1 31 0.7196382206739322 1 32 0.4254730233728523 1 33 1.0061431956807176 1 34 0.8085147782044864 1 35 0.6742148619798414 1 36 1.1733183139516328 1 37 1.4901034325675013 1 38 1.030451145968211 1 39 1.116307135794056 1 40 1.1757815305537278 1 41 1.173046771403996 1 42 0.9008857192758271 1 43 0.9859035997460026 1 44 1.6311599781427049 1 45 0.4903119283281647 1 46 1.9753790163192306 1 47 2.1488610385748714 1 48 2.154119544131163 1 49 2.168693867446205 1 50 2.062047087902723 1 51 2.1212845866491676 1 52 2.264027735978658 1 53 2.6362872277036793 1 54 2.3931164084464207 1 55 2.140473208886295 1 56 1.406045324787403 2 1 -0.44895343643042196 2 2 -0.9714532077352374 2 3 -0.6715991909072585 2 4 -0.7356594711599485 2 5 -2.238167407986385 2 6 -0.7928958382878619 2 7 -0.39289817512987923 2 8 -0.4108114909266405 2 9 -0.5821463967933624 2 10 -0.6423063525027146 2 11 -1.0152329831538367 2 12 -1.1341185325290581 2 13 -0.6379361793518967 2 14 -0.8372029128104899 2 15 -0.8470604953274261 2 16 -0.5230103552260346 2 17 -1.229506994643188 2 18 -0.1518922321723366 2 19 -0.619810377963212 2 20 -0.3851788052000771 2 21 -0.8530143461163178 2 22 0.04220121637622043 2 23 -0.232547055271084 2 24 -0.39313442061425263 2 25 0.25889648826669287 2 26 0.8793297609237456 2 27 1.2420344605419658 2 28 1.1642445153912746 2 29 0.06994425306868735 2 30 0.6300325439970997 2 31 0.48685182008265215 2 32 1.1664634150949489 2 33 0.29128731984923206 2 34 0.310126012498646 2 35 0.1384425042562647 2 36 0.7920175009435013 2 37 0.5642941199289204 2 38 0.3383447158130011 2 39 0.14687624148511347 2 40 -0.2891999919887702 2 41 0.619329106954627 2 42 0.944359481286174 2 43 0.7277069995981833 2 44 1.031588655156746 2 45 1.0080207235979373 2 46 1.4499983806946908 2 47 1.0494227532829257 2 48 1.5660042447963534 2 49 0.8138971194787246 2 50 1.4690093938894186 2 51 0.5560961165826477 2 52 0.7709161321353477 2 53 0.9634564812143971 2 54 0.2047645976555863 2 55 0.4106432014953114 2 56 0.12030992197882995 3 1 -0.3284065036883703 3 2 -0.39037412421712503 3 3 -0.38515425373061163 3 4 -0.318497608632228 3 5 -0.6412864028126961 3 6 -0.26284410140685566 3 7 -0.33638671910622975 3 8 -0.31984327215354824 3 9 -0.4008603657512282 3 10 -0.1797342904184589 3 11 -0.6050482085788147 3 12 -0.21756273060740483 3 13 -0.1208334446881878 3 14 -0.7254069039413054 3 15 0.4465389133939368 3 16 -0.1483022990545635 3 17 0.0151345426812739 3 18 -0.5166372432588062 3 19 -0.198273053499058 3 20 -0.14101029078766203 3 21 0.30671957849565956 3 22 -0.13127993661712622 3 23 -0.1350895534233345 3 24 1.0560747442046718 3 25 0.604262341925228 3 26 0.16169487548398465 3 27 0.056772080645441036 3 28 0.018479548650695676 3 29 0.1714815775328411 3 30 0.1010007627701981 3 31 -0.040361779326069325 3 32 -0.07998152208169529 3 33 0.11523410477162699 3 34 0.4521511418408508 3 35 -0.056694228929249135 3 36 0.6678637684970852 3 37 0.8028307052947738 3 38 1.0049966005136675 3 39 0.6108525903395119 3 40 0.7757815305537292 3 41 0.8166831350403605 3 42 0.8827039010940112 3 43 0.8659035997460052 3 44 1.1947963417790675 3 45 0.930311928328166 3 46 1.42628810722832 3 47 1.381588311302142 3 48 1.3650286350402538 3 49 1.626875685628022 3 50 2.1638652697209046 3 51 1.721284586649169 3 52 1.5149368268877517 3 53 1.7162872277036811 3 54 1.7858436811736915 3 55 1.722291390704477 3 56 0.7514998702419486 4 1 -0.18211920320259622 4 2 -0.671012569741066 4 3 -0.3506781954377587 4 4 -0.4047155128654012 4 5 -1.0141247095441344 4 6 0.02721835522048366 4 7 -0.06825347830123718 4 8 -0.027518497691367827 4 9 -0.08903524430778731 4 10 0.04238923491117674 4 11 0.5222522771949514 4 12 0.3863928557909304 4 13 0.5692567704401981 4 14 0.07631412312323249 4 15 0.8446790938241904 4 16 0.6989843074686846 4 17 0.8971609132572596 4 18 1.2186160530631938 4 19 0.7885555377570128 4 20 0.7552978285738945 4 21 1.1168236728745597 4 22 1.0178109724737823 4 23 1.0301290447723588 4 24 0.2589477490624077 4 25 0.7144913495588199 4 26 0.3255533071286756 4 27 0.8465153145108104 4 28 0.8695135528144711 4 29 0.9859437565751747 4 30 0.7690507280720702 4 31 1.8625112255316694 4 32 1.8608095951979706 4 33 1.3570106488382478 4 34 1.9226438552343268 4 35 2.294062190224114 4 36 1.8427839628065925 4 37 1.5892429190352324 4 38 1.9244553097433688 4 39 2.163788051824593 4 40 2.407814840754977 4 41 2.4020821634928247 4 42 2.7923083424541773 4 43 2.873148568517692 4 44 3.0771419351170266 4 45 3.146883753449611 4 46 3.3907294673948734 4 47 3.4615744320516235 4 48 3.291288177024988 4 49 3.8093045544691044 4 50 3.2103052419624056 4 51 3.7320410058025324 4 52 3.399725168317314 4 53 3.2983413567807105 4 54 3.53573958679479 4 55 3.7590575253332084 4 56 2.1088905711440997 5 1 -0.24970710090137516 5 2 -0.6238180525515702 5 3 -0.18020402148111003 5 4 -0.1499375555466287 5 5 -0.505599607855828 5 6 -0.016354386741959104 5 7 -0.07045440324690588 5 8 -0.028549310640606507 5 9 -0.015206749294691235 5 10 0.23609848993987015 5 11 -0.03279870625632242 5 12 0.045277349020999225 5 13 -0.17170272139688336 5 14 0.21424803965523864 5 15 0.04404389016898591 5 16 0.2590500566189604 5 17 0.26530043518292956 5 18 -0.013651178228947458 5 19 0.001488061298552168 5 20 -0.03970969357465748 5 21 0.5162219009906792 5 22 -0.07342990343862965 5 23 -0.04573321632977212 5 24 -0.3366392571224708 5 25 0.23842292586683556 5 26 0.35959799426301586 5 27 0.24624786034019763 5 28 0.5305432513713342 5 29 0.5197098456151252 5 30 0.32251370238532573 5 31 0.4676541463540893 5 32 0.021783574135955774 5 33 0.36659442328523184 5 34 0.058627585105615054 5 35 0.31947033643239564 5 36 0.004705175265499406 5 37 -0.23337367426726985 5 38 0.468752406751225 5 39 0.06842392411522624 5 40 0.3862792080587063 5 41 1.0121841303953723 5 42 0.47824471064941676 5 43 0.2493674351806412 5 44 0.6552608407837113 5 45 0.46392838486433163 5 46 -0.11085854174314491 5 47 -0.20068109812055113 5 48 -0.2248983722590161 5 49 -0.4127659865683917 5 50 -0.14546452457239312 5 51 0.2720476921568 5 52 0.4946713988850959 5 53 0.7245685813864959 5 54 0.7129836944450929 5 55 0.7126430828079933 5 56 0.7763837454907865 6 1 -0.37883532015491994 6 2 -0.40786983605246263 6 3 -0.15283864481122578 6 4 -0.22470687106790876 6 5 -0.3213550134473451 6 6 0.2895401181471726 6 7 0.167970056193937 6 8 0.1448565563198656 6 9 0.1262751402521971 6 10 0.08963106121104403 6 11 0.13066362675566268 6 12 0.2774629983805923 6 13 -0.009066720845993359 6 14 -0.22482371354679742 6 15 0.30753376759633255 6 16 0.018284321871675502 6 17 0.6357177330757864 6 18 0.6614073536537148 6 19 0.7690682844083199 6 20 0.4309279596411528 6 21 0.3596869884441958 6 22 0.6140373875681249 6 23 0.6982552150157701 6 24 1.3842394097278259 6 25 0.5725642287176811 6 26 0.7249195753124553 6 27 0.17814429333840565 6 28 0.16585519187539788 6 29 0.745615368270407 6 30 0.7838137987907814 6 31 1.1670481692159562 6 32 1.2024198501309975 6 33 1.8047709829877512 6 34 2.088926442012376 6 35 1.0930827865081412 6 36 1.6978466158384258 6 37 1.224717497747605 6 38 1.4151509744416266 6 39 1.3985712867374538 6 40 1.5258329885297144 6 41 1.5097877662581993 6 42 1.4026352904593615 6 43 1.1553032566928323 6 44 1.518089652242189 6 45 1.7382707619473763 6 46 0.8535265291837248 6 47 1.4177461157618332 6 48 1.1182361822100653 6 49 1.1000146221631866 6 50 0.5428704155185038 6 51 1.4989861303884489 6 52 1.6261546656527592 6 53 1.9905925450278659 6 54 0.8362724976402447 6 55 0.9130975656615945 6 56 0.7786010709280546 7 1 -0.04560091975918823 7 2 -0.411756487172525 7 3 -0.3018175155624121 7 4 -0.17810945745755902 7 5 -0.48613488766117996 7 6 0.1203223950180643 7 7 -0.022065983661903488 7 8 0.01916932573545438 7 9 0.12105996111291972 7 10 -0.0692269700235002 7 11 0.22780163820361565 7 12 -0.052223949538287684 7 13 0.04581279296928642 7 14 0.026367015023623708 7 15 0.1908494343336713 7 16 -0.006293446483915943 7 17 -0.31309153835379533 7 18 0.4741697026043141 7 19 -0.43094584886848253 7 20 -0.36928403951085187 7 21 0.1652554995034876 7 22 -0.3454849076760311 7 23 0.4149819481088386 7 24 0.1728197220732426 7 25 -0.6089211786740201 7 26 -8.925798554315634E-5 7 27 0.02430357536794503 7 28 -0.06225589568025214 7 29 -0.13444964480287425 7 30 -0.19654094645690634 7 31 0.029192187306549044 7 32 0.2985543989261359 7 33 0.36235701931027364 7 34 0.5472686086437086 7 35 0.9715931391334003 7 36 1.0362396622662402 7 37 0.6741415667179957 7 38 1.0482652419845557 7 39 0.8243221170674637 7 40 0.18481115261705838 7 41 0.8748785725616415 7 42 1.3861700229870983 7 43 1.4634793573217628 7 44 0.8327670601992239 7 45 0.9478672568947317 7 46 1.0688894010655687 7 47 0.9046799013600264 7 48 0.8552159009578588 7 49 0.6722144666971417 7 50 0.49700793230177 7 51 0.8346655876706173 7 52 0.7717839498022876 7 53 0.7093686032569657 7 54 0.7419894081059368 7 55 0.5307217618314795 7 56 0.7348161794009549 8 1 -0.07087498411305404 8 2 0.21559800584259392 8 3 -0.1473573061526423 8 4 -0.2119813511670685 8 5 0.2170281293704477 8 6 0.06028794902446322 8 7 0.013487202592507685 8 8 -0.08329383618805508 8 9 -0.07360090987863543 8 10 0.5390181979690638 8 11 0.24092392148090624 8 12 0.13286858989690842 8 13 0.6494651618147955 8 14 0.046364827976411505 8 15 -0.4121604893930595 8 16 -0.09131490688469057 8 17 0.26530043518292956 8 18 0.45715174147908044 8 19 -0.16639515038028208 8 20 0.1646698684691401 8 21 0.16950657252352386 8 22 -0.06978026840212692 8 23 -0.1880689827531299 8 24 0.656061472804538 8 25 0.5267440937500467 8 26 0.1661673373287087 8 27 -0.22820469440432944 8 28 0.11083522217425212 8 29 0.03430838576111128 8 30 -0.29427461878255556 8 31 0.11363954781394625 8 32 -0.4344208054260861 8 33 -0.11150776649579086 8 34 -0.18954759737613713 8 35 0.2318790955564829 8 36 0.17623802198082927 8 37 0.6096920191633899 8 38 0.48700058193370666 8 39 0.8129494715604793 8 40 0.05051278470104137 8 41 1.0194834004683635 8 42 1.0074417909413889 8 43 0.9719951724069205 8 44 1.093217045163275 8 45 0.12451232647017108 8 46 -0.39917970962635607 8 47 0.328515982171421 8 48 -0.28694216787945237 8 49 -0.149992263940657 8 50 0.24504642433271684 8 51 1.3778871082151944 8 52 0.8194889171332704 8 53 0.9544955886857629 8 54 1.227582234591079 8 55 1.136000747041571 8 56 0.8785735265126853 9 1 -0.4076678673247329 9 2 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119 21 0.469391458586891 119 22 0.48017656387163044 119 23 0.8113816098220212 119 24 0.7851591365148707 119 25 0.6829720193445823 119 26 0.46670627985022506 119 27 0.5310894478660337 119 28 0.9675052899345005 119 29 1.4307712484354163 119 30 1.0193585340311948 119 31 1.4191885628049192 119 32 1.326704694927102 119 33 1.2891018141232102 119 34 1.9227669776179752 119 35 1.4333546469260767 119 36 1.6170459125179413 119 37 1.8805367984847408 119 38 2.3982712828206054 119 39 2.1968806124965674 119 40 1.7825720160538907 119 41 1.8730663217461299 119 42 1.5539844485035879 119 43 1.7753073143110072 119 44 2.109960890492001 119 45 1.4434171743366377 119 46 1.5435380257685622 119 47 1.9341591812923689 119 48 1.9082544414918665 119 49 2.0119913584856306 119 50 2.224888404292429 119 51 2.3139857922536002 119 52 2.1321802286472824 119 53 2.2894902254228064 119 54 1.7458958154193738 119 55 2.803203740003923 119 56 1.5533897366479437 120 1 -0.7345625523641033 120 2 -1.9031157133366676 120 3 -0.7304942680470035 120 4 -1.0354482170789012 120 5 -0.9060823942228637 120 6 -0.5057503290374967 120 7 -0.9774318157418804 120 8 -0.8587981755178973 120 9 -1.0738882827161547 120 10 -0.1061050849782319 120 11 -0.05495515202906631 120 12 -0.12550832831677639 120 13 -0.2102106816244813 120 14 0.17921729076162762 120 15 0.060883938447624075 120 16 0.19384515978580907 120 17 0.20590046966767517 120 18 0.38565337950425516 120 19 0.03568542896336169 120 20 0.5750670177305892 120 21 0.38733518336322703 120 22 0.36033065751315263 120 23 0.36552605144423467 120 24 0.9746717377622929 120 25 0.8403253340512151 120 26 1.1449017473522751 120 27 1.0498572631794403 120 28 0.8271982315426065 120 29 1.3120542332236091 120 30 1.300156095626317 120 31 1.857762773286673 120 32 1.7844136103449344 120 33 1.9117981706270335 120 34 2.0925663172166544 120 35 1.8742148619798407 120 36 2.365444298203599 120 37 2.096846453326272 120 38 2.3611025418164573 120 39 2.189063041305868 120 40 2.3143785241113513 120 41 2.5643710376888933 120 42 2.7478291695263657 120 43 3.0094111158519468 120 44 3.0590196774984655 120 45 2.778457955529312 120 46 4.013918744307777 120 47 4.1714379891833175 120 48 4.064083753150491 120 49 3.9965678831942384 120 50 4.467473000572731 120 51 3.0602251736212516 120 52 3.437742839772504 120 53 3.317275058770253 120 54 3.0014485487470637 120 55 3.3344316913487155 120 56 2.2049715953672173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 507.0 3 43.0 4 118.0 5 208.0 6 491.0 7 1327.0 8 629.0 9 910.0 10 1389.0 11 1526.0 12 2060.0 13 2851.0 14 3291.0 15 3710.0 16 4803.0 17 5157.0 18 5305.0 19 6316.0 20 5869.0 21 4909.0 22 5613.0 23 6650.0 24 7368.0 25 7799.0 26 7971.0 27 8554.0 28 9790.0 29 11134.0 30 12609.0 31 14786.0 32 18010.0 33 21451.0 34 25300.0 35 28311.0 36 29132.0 37 23759.0 38 7228.0 39 894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 57.99824029982067 27.600762984505234 4.852944139593926 9.548052576080167 11 31.247103546937648 26.423039982805985 16.697002464923532 25.632854005332835 12 38.27851621006253 12.80484119041702 28.659941298551274 20.25670130096918 13 20.857484434713108 14.439951910483648 31.535573480915314 33.166990173887925 14 20.869238157284958 21.70778230762514 37.804337459449656 19.618642075640242 15 29.045463398907913 13.698459926522444 35.39381687028592 21.862259804283728 16 25.308115441704892 16.00689103963355 34.04045967129875 24.6445338473628 17 33.917885135906616 15.31677961434357 22.23972220916253 28.525613040587285 18 32.900012761184506 16.63420400432537 26.52983094788735 23.93595228660277 19 29.692253961004507 18.876478450389218 29.105911114991706 22.325356473614573 20 28.006098502911563 24.412481781730015 25.073712631557736 22.507707083800685 21 27.696136047659664 18.365023608191336 31.946282129640203 21.992558214508794 22 30.437074972714296 17.641507849886658 25.340273696582987 26.581143480816056 23 33.57882646892883 23.609320007522637 22.99206093334408 19.819792590204454 24 31.375387033293258 24.112929766470323 23.698862911296334 20.81282028894008 25 28.20415498043431 21.79097458979225 20.2599801823892 29.74489024738424 26 31.7962437653765 17.724143957624726 24.856486025032602 25.623126251966177 27 23.951809982890122 18.90960674176188 21.99745200662882 35.14113126871918 28 27.185096024530637 27.285289687449566 21.18961482597235 24.339999462047448 29 35.272178165210434 18.881960839706466 22.16572162694809 23.68013936813501 30 28.029053603764314 23.542439398733112 25.24452552287736 23.183981474625217 31 35.197629789239784 19.10982425425897 24.009157632482662 21.683388324018583 32 33.677249033578924 16.367873065474186 28.307719246258905 21.647158654687985 33 36.593894112059175 15.890938856906095 25.826228436877862 21.688938594156863 34 29.705848666347766 19.77066167206775 30.875110323593418 19.648379337991067 35 25.58942075677716 26.775002359031046 30.215143631290186 17.420433252901606 36 30.276909379710848 26.31465350074025 24.71713829955855 18.691298819990354 37 28.38057417171213 23.32083765962324 28.918270203212014 19.38031796545261 38 29.36909336905288 23.57490139447944 27.117952109264028 19.938053127203652 39 28.866417804659072 21.510587885865494 28.106668646762206 21.516325662713225 40 28.23020805187787 21.415158065387732 28.20521480680897 22.149419075925426 41 29.045880051754835 20.336068592045567 29.172226707791264 21.445824648408333 42 24.904368630207347 21.45859803736044 29.996147711605524 23.640885620826687 43 27.562211249564868 17.87371530347734 28.156356987633792 26.407716459324 44 26.998648191956743 17.876985468063538 28.802636025684354 26.321730314295372 45 26.796731586438177 20.46139127318704 29.364674223548942 23.37720291682584 46 25.98833572817175 21.426084016566644 28.821908545346968 23.76367170991463 47 26.405999472306224 20.864876567013727 29.412839195469953 23.316284765210096 48 27.388336734244977 19.48062495558511 28.618417713166096 24.512620597003814 49 25.531943678740177 21.227689820436243 29.30484575239886 23.935520748424715 50 25.246375438264003 20.358124297762316 30.58947354171461 23.806026722259073 51 21.640849382448756 21.999776727570424 32.46042834477323 23.898945545207592 52 24.03213085232062 20.374927644651457 33.95776140167966 21.635180101348265 53 21.665651025797278 20.291827476470985 33.913941363667135 24.128580134064595 54 22.830271082807723 21.561696984470377 32.45646999874734 23.15156193397456 55 21.76381324011598 21.393585887326232 29.921347857897736 26.921253014660056 56 22.649228205874984 21.284788306929553 26.840080464363364 29.2259030228321 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 81.5 11 163.0 12 189.0 13 248.0 14 281.0 15 332.5 16 384.0 17 724.0 18 1064.0 19 1429.5 20 1795.0 21 1682.0 22 1930.5 23 2292.0 24 2720.5 25 3149.0 26 3776.0 27 4403.0 28 4961.0 29 5757.0 30 5995.0 31 6278.5 32 6562.0 33 5960.5 34 5359.0 35 7585.5 36 9812.0 37 7358.5 38 4659.0 39 4413.0 40 4133.0 41 3853.0 42 4147.0 43 4441.0 44 5400.5 45 6360.0 46 7176.0 47 7437.0 48 6882.0 49 6770.0 50 6658.0 51 7759.5 52 8861.0 53 10705.5 54 12883.0 55 13216.0 56 14400.5 57 15585.0 58 18815.5 59 22046.0 60 22541.5 61 23037.0 62 24651.5 63 24246.5 64 22227.0 65 20304.5 66 18382.0 67 15543.0 68 12704.0 69 10953.5 70 9203.0 71 7744.5 72 5455.0 73 4624.0 74 3834.5 75 3045.0 76 2652.5 77 2260.0 78 1792.0 79 1128.0 80 932.0 81 762.5 82 593.0 83 448.0 84 303.0 85 227.0 86 151.0 87 108.0 88 49.0 89 33.0 90 21.5 91 10.0 92 6.5 93 3.0 94 4.5 95 6.0 96 3.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0010074619347298995 23 0.003358206449099665 24 0.0 25 0.02115670062932789 26 0.08261187864785176 27 0.09705216637898031 28 0.11888050829812814 29 0.14641780118074538 30 0.22567147337949747 31 0.2545520488417546 32 0.270671439797433 33 0.30794753138243924 34 0.31029827589680903 35 0.3512683945758249 36 0.4214549093620079 37 0.3835071764871817 38 0.4684697996494032 39 0.5023876847853098 40 0.570223455057123 41 0.5933950795559108 42 0.6212681930834381 43 0.6350368395247467 44 0.6306711711409171 45 0.6639174149870037 46 0.6726487517546629 47 0.7226860278462479 48 0.7619770433007139 49 0.710932305274399 50 0.770372559423463 51 0.730409902679177 52 0.7928725426324309 53 0.8052979064940997 54 0.8083202922982893 55 0.8576859271000544 56 0.8368650471156364 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 297778.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.75852613149911 #Duplication Level Percentage of deduplicated Percentage of total 1 72.05892931327462 25.046621775433024 2 11.234273439427305 7.8097357382548065 3 4.89302190786607 5.1022268953968135 4 2.8767429015848918 3.999653732733721 5 1.8529309154246825 3.2202573821825697 6 1.2718666604668 2.652492633217067 7 0.9693716249713524 2.3585750270390355 8 0.7407554404013014 2.0598053865791015 9 0.5540690767385037 1.733276203422378 >10 3.0698421675992975 19.603891621915107 >50 0.27553705306821547 6.574304463249573 >100 0.18269304605609937 11.75817094569892 >500 0.011979871872531108 2.6483211054323204 >1k 0.007986581248354071 5.4326670894455455 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGGTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 4889 1.6418271329648262 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 2391 0.8029471619797298 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1802 0.6051488021277596 No Hit GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 1791 0.60145477503375 No Hit GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 1369 0.45973846288174414 No Hit GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1223 0.410708648724889 No Hit GTGGTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1107 0.3717534539153329 No Hit GTGGTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1084 0.3640295790824037 No Hit GTGGTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 776 0.260596820450134 No Hit GTGGTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 752 0.2525371249722948 No Hit GTGGTAGGGAGCTGCATTGAGAAGAGAGACAAGAGATCCAGTGAGCCTCTCTACCC 736 0.2471639946537353 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 695 0.2333953482124267 No Hit GTGGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 671 0.2253356527345875 No Hit GTGGTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 622 0.20888044113399917 No Hit GTGGTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 613 0.20585805532980944 No Hit GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.2014923869459799 No Hit GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 596 0.20014910436634004 No Hit GTGGTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 533 0.17899240373701214 No Hit GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 532 0.17865658309210217 No Hit GTGGTAGGGACTCTATGTGCTTATAATATAGCCTTGCTGCTACTCGTGAACCTACG 506 0.16992524632444306 No Hit GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGGCCACGCCGAGCGAC 482 0.16186555084660384 No Hit GTGGTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 441 0.14809690440529522 No Hit GTGGTAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 439 0.1474252631154753 No Hit GTGGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 424 0.1423879534418258 No Hit GTGGTAGGGCACTGCTCACAACAAAAACAGACAAGGCCCACAGGTGCATCAGTTAG 404 0.13567154054362646 No Hit GTGGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA 396 0.13298497538434673 No Hit GTGGTAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 379 0.1272760244208773 No Hit GTGGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 360 0.12089543216758794 No Hit GTGGTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 358 0.120223790877768 No Hit GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGGGGTCCACGCCGAGCGAC 352 0.11820886700830821 No Hit GTGGTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 351 0.11787304636339824 No Hit GTGGTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 346 0.1161939431388484 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 337 0.11317155733465871 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC 335 0.11249991604483876 No Hit GTGGTAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGGGGGCCACGCCGAGCGAC 333 0.11182827475501883 No Hit GTGGTAGGGCATATTATTACAGTATTCAGCATGCCCTATGATATATAGAAATGCAC 332 0.11149245411010886 No Hit GTGGTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 327 0.10981335088555903 No Hit GTGGTAGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTA 326 0.10947753024064907 No Hit GTGGTAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCC 325 0.1091417095957391 No Hit GTGGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTC 314 0.10544768250172949 No Hit GTGGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 314 0.10544768250172949 No Hit GTGGTAGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC 308 0.10343275863226968 No Hit GTGGTAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 300 0.10074619347298995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.011753722571848826 0.0 0.0 0.0 0.0 11 0.02955221675207705 0.0 0.0 0.0 0.0 12 0.030895499331716918 0.0 0.0 0.0 0.0 13 0.031231319976626884 0.0 0.0 0.0 0.0 14 0.04902981415685511 0.0 0.0 0.0 0.0 15 0.05003727609158501 0.0 0.0 0.0 0.0 16 0.05003727609158501 0.0 0.0 0.0 0.0 17 0.051380558671224874 0.0 0.0 0.0 0.0 18 0.05171637931613484 0.0 0.0 0.0 0.0 19 0.05171637931613484 0.0 0.0 0.0 0.0 20 0.0671641289819933 0.0 0.0 0.0 0.0 21 0.06749994962690327 0.0 0.0 0.0 0.0 22 0.06951487349636307 0.0 0.0 0.0 0.0 23 0.07824621026402219 0.0 0.0 0.0 0.0 24 0.08731336767659129 0.0 0.0 0.0 0.0 25 0.08764918832150126 0.0 0.0 0.0 0.0 26 0.09100739477060092 0.0 0.0 0.0 0.0 27 0.09369395992988065 0.0 0.0 0.0 0.0 28 0.14238795344182578 0.0 0.0 0.0 0.0 29 0.14608198053583543 0.0 0.0 0.0 0.0 30 0.16992524632444303 0.0 0.0 0.0 0.0 31 0.17194017019390284 0.0 0.0 0.0 0.0 32 0.17227599083881281 0.0 0.0 0.0 0.0 33 0.1732834527735427 0.0 0.0 0.0 0.0 34 0.17563419728791246 0.0 0.0 0.0 0.0 35 0.2243281907998576 0.0 0.0 0.0 0.0 36 0.2273505766040473 0.0 0.0 0.0 0.0 37 0.22902967982859715 0.0 0.0 0.0 0.0 38 0.23171624498787688 0.0 0.0 0.0 0.0 39 0.23507445143697653 0.0 0.0 0.0 0.0 40 0.23776101659625626 0.0 0.0 0.0 0.0 41 0.24011176111062604 0.0 0.0 0.0 0.0 42 0.2434699675597257 0.0 0.0 0.0 0.0 43 0.2464923533639154 0.0 0.0 0.0 0.0 44 0.24817145658846523 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTATCA 15 0.00827616 50.22576 48 AACTTAG 15 0.00827616 50.22576 50 CCAATCG 15 0.00827616 50.22576 48 ACTTAGC 25 2.0288882E-5 50.22576 47 TTAACTA 15 0.00827616 50.22576 47 AACCTAC 60 0.0 50.22576 49 ACCAATC 15 0.00827616 50.22576 47 AACTATT 15 0.00827616 50.22576 49 ATAGATC 20 4.086955E-4 50.225758 50 TTATCTC 40 2.566594E-9 50.225758 47 AGATTAC 20 4.086955E-4 50.225758 48 TGCACGT 20 4.086955E-4 50.225758 49 TGCATGA 15 0.008281698 50.21726 46 CGAATCT 25 2.0309208E-5 50.21726 46 TACAATG 20 4.0903696E-4 50.21726 46 TCGTGAA 60 0.0 50.208767 44 TTCTTAC 35 5.1013558E-8 50.208767 45 CATAAGC 15 0.00828724 50.208767 45 AATTATC 40 2.57387E-9 50.208763 45 CGTTGAC 20 4.0972058E-4 50.20027 43 >>END_MODULE