FastQCFastQC Report
Tue 31 May 2016
SRR212706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8056
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5626.976166832174776No Hit
TCGGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4695.8217477656405165No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2072.5695134061569016No Hit
TCGGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1091.3530287984111222No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA911.1295928500496524No Hit
TCGGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT851.0551142005958292No Hit
TCGGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC811.0054617676266138No Hit
TCGGGTGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA570.7075471698113208No Hit
TCGGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT500.6206554121151936No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.5337636544190666No Hit
TCGGGTGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT220.2730883813306852No Hit
TCGGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA190.2358490566037736No Hit
TCGGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.21102284011916583No Hit
TCGGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.21102284011916583No Hit
TCGGGTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.19860973187686196No Hit
TCGGGTGGGGGGAATTCCTTCGATTCTGCTTTGCTACCCGTTGTAGCGCCGGAAGATG160.19860973187686196No Hit
TCGGGTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG150.1861966236345581No Hit
TCGGGTGGGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACT150.1861966236345581No Hit
TCGGGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC110.1365441906653426No Hit
TCGGGTGGGTACTGAACAATTTCAAATACACAAATGAACAAATTTTCACTATGTATTG110.1365441906653426No Hit
TCGGGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT110.1365441906653426No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT110.1365441906653426No Hit
TCGGGTGGGCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACTC100.12413108242303873No Hit
TCGGGTGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.12413108242303873No Hit
TCGGGTGGGACCACTGCTAATAGAATCGTGTTTAACTATAAGCTGACAAAGCCTTGCG100.12413108242303873No Hit
TCGGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12413108242303873No Hit
TCGGGTGGGAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTA100.12413108242303873No Hit
TCGGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGG100.12413108242303873No Hit
TCGGGTGGGAAGCTGGTACAACGAGAGTCGATCTTTTTTTTTTTTTTTTTTTTTTTTT90.11171797418073487No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC90.11171797418073487Illumina Single End Adapter 2 (96% over 25bp)
TCGGGTGGGGGCATGGTGGCGCATGCCTGTGATCCCAGCTACTCGGGAGGCTGAGGCA90.11171797418073487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGCA150.00696379552.00000438
GAAGCAT150.00696379552.0000049
TGGGGCC150.00696379552.0000046
GTGGGGT600.052.0000045
GGGGGCC150.00696379552.0000047
GTGGGAT307.041708E-752.0000045
GGCTCCC150.00696379552.00000432
TCGTCTG150.00696379552.00000435
GGGATGG150.00696379552.0000047
TGGGTGG150.00696379552.0000046
GGGAAGT150.00696379552.0000047
GGGAAAT150.00696379552.0000047
GCTCCCC150.00696379552.00000433
TGGGAGT353.277637E-852.0000046
TGGGACT150.00696379552.0000046
TGGGAAG1200.052.0000046
TGGGAAC150.00696379552.0000046
TGGGAAA150.00696379552.0000046
CTCGTCT150.00696379552.00000434
CGTCTGG150.00696379552.00000436
Produced by FastQC (version 0.11.5)