FastQCFastQC Report
Tue 31 May 2016
SRR212705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8482
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5916.967696298042915No Hit
TCGGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4675.505776939401084No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2052.4168828106578637No Hit
TCGGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1081.2732846026880453No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1021.202546569205376No Hit
TCGGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT911.0728601744871493No Hit
TCGGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC700.8252770572978071No Hit
TCGGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT670.7899080405564726No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.6248526290969111No Hit
TCGGGTGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA420.4951662343786843No Hit
TCGGGTGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT220.25937278943645364No Hit
TCGGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA190.2240037726951191No Hit
TCGGGTGGGCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACTC170.200424428200896No Hit
TCGGGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC160.18863475595378448No Hit
TCGGGTGGGGAAGAGAGCAATGAGCACAGATGGTCCCGTGGCACGTACCATCCTGAAG150.17684508370667296No Hit
TCGGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.17684508370667296No Hit
TCGGGTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.16505541145956143No Hit
TCGGGTGGGGTGCCGTTCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGG130.15326573921244988No Hit
TCGGGTGGGAAGCTGGTACAACGAGAGTCGATCTTTTTTTTTTTTTTTTTTTTTTTTT130.15326573921244988No Hit
TCGGGTGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT120.14147606696533838No Hit
TCGGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA110.12968639471822682No Hit
TCGGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.11789672247111531No Hit
TCGGGTGGGTACTGAACAATTTCAAATACACAAATGAACAAATTTTCACTATGTATTG100.11789672247111531No Hit
TCGGGTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG100.11789672247111531No Hit
TCGGGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT100.11789672247111531No Hit
TCGGGTGGGGCCTCCTTCCTTTTGGCCTCTCTCCTCTATTCCCGTAACTCCCCGGTAC90.10610705022400377No Hit
TCGGGTGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.10610705022400377No Hit
TCGGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10610705022400377No Hit
TCGGGTGGGATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGA90.10610705022400377No Hit
TCGGGTGGGGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCAT90.10610705022400377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAAC800.052.23809452
GTGGGCA457.2759576E-1151.9289975
GTGGGAG457.2759576E-1151.9289975
GTGGGAC457.2759576E-1151.9289975
TCCTCTG150.00701369551.92899335
GACAGTG150.00701369551.9289939
CTCCTCT150.00701369551.92899334
GAAGTGG150.00701369551.9289939
GGACAGT150.00701369551.9289938
GGGAGTG150.00701369551.9289937
TGGGGGT307.1521936E-751.9289936
TGGGGGA251.5308282E-551.9289936
TGGGGCA203.277376E-451.9289936
GTGGGTG401.5588739E-951.9289935
GTGGGTC203.277376E-451.9289935
GTGGGGC307.1521936E-751.9289935
GTGGGGA203.277376E-451.9289935
GTGGGAT353.340392E-851.9289935
GGCTCCT150.00701369551.92899332
GGAAGTG150.00701369551.9289938
Produced by FastQC (version 0.11.5)