FastQCFastQC Report
Tue 31 May 2016
SRR212704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8962
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5345.958491408167819No Hit
TCGGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4955.523320687346574No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2012.2428029457710332No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1001.115822361080116No Hit
TCGGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT890.9930819013613033No Hit
TCGGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC890.9930819013613033No Hit
TCGGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA850.9484490069180985No Hit
TCGGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT610.6806516402588707No Hit
TCGGGTGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA420.46864539165364877No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.46864539165364877No Hit
TCGGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA380.4240124972104441No Hit
TCGGGTGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAT210.23432269582682438No Hit
TCGGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.21200624860522205No Hit
TCGGGTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT190.21200624860522205No Hit
TCGGGTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG160.17853157777281856No Hit
TCGGGTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATG140.15621513055121625No Hit
TCGGGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC130.1450569069404151No Hit
TCGGGTGGGAAGCTGGTACAACGAGAGTCGATCTTTTTTTTTTTTTTTTTTTTTTTTT120.13389868332961394No Hit
TCGGGTGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG120.13389868332961394No Hit
TCGGGTGGGGGGAATTGCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG110.12274045971881276No Hit
TCGGGTGGGAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTA110.12274045971881276No Hit
TCGGGTGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATA110.12274045971881276No Hit
TCGGGTGGGACCACTGCTAATAGAATCGTGTTTAACTATAAGCTGACAAAGCCTTGCG100.11158223610801161No Hit
TCGGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.11158223610801161No Hit
TCGGGTGGGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACT100.11158223610801161No Hit
TCGGGTGGGACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG90.10042401249721045No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10042401249721045No Hit
TCGGGTGGGTGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT90.10042401249721045No Hit
TCGGGTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10042401249721045No Hit
TCGGGTGGGGGGAATTCCTTCGATTCTGCTTTGCTACCCGTTGTAGCGCCGGAAGATG90.10042401249721045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGTG353.2456228E-852.174166
GTGGGAG550.052.174165
GTGGGAC353.2456228E-852.174165
GGATGGT203.2114907E-452.1741568
GACAGTG150.006896411552.1741569
GAAGCAT150.006896411552.1741569
GAGCATG150.006896411552.1741569
GCTATCA150.006896411552.1741569
TGGGGTT251.495351E-552.1741566
TGGGGGT203.2114907E-452.1741566
TGGGGGA401.5115802E-952.1741566
TGGGGCG203.2114907E-452.1741566
TGGGGCC150.006896411552.1741566
GTGGGTG150.006896411552.1741565
TGGGGAT203.2114907E-452.1741566
TGGGGAC150.006896411552.1741566
GTGGGGT750.052.1741565
GTGGGGA650.052.1741565
GTGGGCT251.495351E-552.1741565
GTGGGCG150.006896411552.1741565
Produced by FastQC (version 0.11.5)