FastQCFastQC Report
Tue 31 May 2016
SRR212703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21396
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9894.622359319498972No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8964.1876986352589265No Hit
TCGGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA8794.108244531688166No Hit
TCGGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG2030.9487754720508507No Hit
TCGGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1790.8366049728921293No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.8132361189007292No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.7992148065058889No Hit
TCGGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1670.7805197233127688No Hit
TCGGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1510.7057393905402879No Hit
TCGGGTGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG750.35053280987100394No Hit
TCGGGTGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG640.29912133108992334No Hit
TCGGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.2336885399140026No Hit
TCGGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1869508319312021No Hit
TCGGGTGGGCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGAC340.1589082071415218No Hit
TCGGGTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC320.14956066554496167No Hit
TCGGGTGGGATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTT280.13086558235184145No Hit
TCGGGTGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.1261918115535614No Hit
TCGGGTGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGAT260.12151804075528137No Hit
TCGGGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG250.1168442699570013No Hit
TCGGGTGGGGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCCCCACGAGGCGCC250.1168442699570013No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG240.11217049915872125Illumina Single End Adapter 2 (96% over 30bp)
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG230.10749672836044122Illumina Single End Adapter 2 (95% over 23bp)
TCGGGTGGGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGA220.10282295756216114No Hit
TCGGGTGGGAAGCTGGTACAACGAGAGTCGATCTTTTTTTTTTTTTTTTTTTTTTT220.10282295756216114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCCG204.0243706E-450.1670650
TGCATTA150.00820935450.1670640
TTACCGT150.00820935450.1670644
CCTGCAT150.00820935450.1670638
ACCGTCG150.00820935450.1670646
CCGTCGG150.00820935450.1670647
CATTACC150.00820935450.1670642
TACCGTC150.00820935450.1670645
CGTCGGC150.00820935450.1670648
GGAGGCC204.0243706E-450.1670649
CCGGGGG150.00820935450.1670639
GCATTAC150.00820935450.1670641
AAGCAGG150.00820935450.1670650
GTCTGGC150.00828623750.04929737
TGCCTGC150.00828623750.04929736
GCCTGCA150.00828623750.04929737
TTGCCTG150.00828623750.04929735
CGTTTGC150.00836365449.93208732
GGATGGT150.00836365449.9320878
GAGTGCG301.0093445E-649.93208729
Produced by FastQC (version 0.11.5)