##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212703_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21396 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.398252009721446 38.0 36.0 38.0 33.0 38.0 2 36.13156664797158 38.0 36.0 38.0 32.0 38.0 3 35.927790241166576 38.0 35.0 38.0 31.0 38.0 4 35.76233875490746 38.0 35.0 38.0 31.0 38.0 5 35.86263787623855 38.0 35.0 38.0 31.0 38.0 6 32.47854739203589 35.0 31.0 36.0 23.0 38.0 7 37.24270891755468 38.0 38.0 38.0 35.0 40.0 8 35.670171994765376 38.0 35.0 38.0 30.0 40.0 9 35.59913067863152 38.0 35.0 38.0 30.0 40.0 10 34.32468685735652 35.0 32.0 38.0 28.0 39.0 11 34.599878481959244 37.0 33.0 38.0 27.0 40.0 12 34.80374836418022 37.0 33.0 38.0 28.0 39.0 13 34.55491680687979 37.0 33.0 38.0 27.0 39.0 14 34.71116096466629 38.0 33.0 38.0 27.0 39.0 15 35.283557674331654 38.0 34.0 38.0 29.0 39.0 16 36.18138904468125 38.0 35.0 39.0 31.0 40.0 17 36.27252757524771 38.0 36.0 39.0 31.0 40.0 18 36.15778650214993 38.0 35.0 39.0 31.0 40.0 19 35.6391381566648 38.0 35.0 39.0 30.0 40.0 20 35.87165825387923 38.0 35.0 39.0 30.0 40.0 21 35.745513180033655 38.0 35.0 39.0 30.0 40.0 22 35.46204898111797 38.0 35.0 39.0 30.0 40.0 23 34.971817162086374 38.0 34.0 39.0 28.0 40.0 24 34.920312207889324 38.0 34.0 39.0 28.0 40.0 25 35.10576743316508 38.0 35.0 39.0 29.0 40.0 26 34.775191624602726 38.0 35.0 39.0 28.0 40.0 27 34.57183585716957 38.0 34.0 39.0 28.0 39.0 28 33.91232005982427 37.0 33.0 39.0 26.0 39.0 29 34.17610768367919 38.0 33.0 39.0 27.0 39.0 30 33.69961675079454 37.0 33.0 39.0 25.0 40.0 31 33.44040942232193 38.0 33.0 39.0 23.0 39.0 32 32.99191437651898 38.0 33.0 39.0 19.0 40.0 33 33.28491306786315 38.0 33.0 39.0 22.0 39.0 34 33.56674144699944 38.0 34.0 39.0 23.0 40.0 35 33.0396803140774 38.0 33.0 39.0 19.0 40.0 36 32.291129183024864 38.0 33.0 39.0 2.0 40.0 37 31.303982052720134 37.0 32.0 39.0 2.0 40.0 38 31.17638810992709 38.0 32.0 39.0 2.0 40.0 39 31.16587212563096 38.0 32.0 39.0 2.0 40.0 40 30.733267900542156 37.0 31.0 39.0 2.0 39.0 41 30.23649280239297 36.0 30.0 39.0 2.0 39.0 42 30.394746681622735 36.0 30.0 39.0 2.0 39.0 43 30.275565526266593 36.0 30.0 39.0 2.0 39.0 44 29.77654701813423 35.0 29.0 39.0 2.0 39.0 45 29.78925967470555 36.0 29.0 39.0 2.0 39.0 46 29.701532996821836 36.0 29.0 39.0 2.0 39.0 47 29.624789680314077 36.0 29.0 39.0 2.0 39.0 48 29.48158534305478 36.0 29.0 39.0 2.0 39.0 49 29.34726117031221 36.0 28.0 39.0 2.0 39.0 50 29.09277435034586 36.0 28.0 39.0 2.0 39.0 51 28.625817909889697 36.0 26.0 39.0 2.0 39.0 52 28.26967657506076 35.0 23.0 39.0 2.0 39.0 53 27.703449242849132 35.0 20.0 39.0 2.0 39.0 54 27.477752851000186 35.0 16.0 39.0 2.0 39.0 55 27.177509814918675 35.0 2.0 39.0 2.0 39.0 56 15.571976070293513 19.0 2.0 24.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.9550977089400376 1 2 -1.2173875512302672 1 3 -1.9411865515509135 1 4 -3.443314803170537 1 5 -4.070978636318035 1 6 -4.410748915383785 1 7 -1.8827969516918373 1 8 -1.5675472778472468 1 9 -1.4684077130242272 1 10 -1.8287102508702162 1 11 -1.1207193610158228 1 12 -1.6279450601893828 1 13 -1.355534641382576 1 14 -2.049344046184004 1 15 -1.253913615826285 1 16 -2.6338617086763207 1 17 -0.9441364016288532 1 18 -0.4066225476962373 1 19 -2.0966759097859864 1 20 -0.753119547847291 1 21 0.47942832635447274 1 22 0.47211652603626675 1 23 0.6477782434861581 1 24 1.4420815647771335 1 25 -0.09659933116694219 1 26 -0.019630077849697614 1 27 0.11696790968945692 1 28 -0.31315987460750705 1 29 -2.0310945074154994 1 30 -0.31286678758192465 1 31 -5.317166574388459 1 32 -5.820956489359961 1 33 -4.534323638452463 1 34 -6.523342590624772 1 35 -6.0173366621252065 1 36 -4.5312604115668265 1 37 -4.549388929040035 1 38 -7.56167791302655 1 39 -8.565959375588122 1 40 -7.669855120488947 1 41 -8.682199176696663 1 42 -8.353398641096376 1 43 -8.685069039812184 1 44 -9.212592962881743 1 45 -8.264813012642893 1 46 -8.242002585817133 1 47 -8.272843977632348 1 48 -7.675059618556116 1 49 -7.737827573096965 1 50 -8.0647170895753 1 51 -7.275264213481623 1 52 -6.76049795985568 1 53 -7.414530149708828 1 54 -7.505319694722807 1 55 -8.259098067409717 1 56 -6.099939811744193 2 1 -0.6950357894353942 2 2 -0.9666135574222139 2 3 -2.913322774461129 2 4 -0.43712285270613904 2 5 -1.4146318870920283 2 6 -1.1166312683249622 2 7 -0.6877505120633529 2 8 -0.5737392283116449 2 9 -0.9451878987827413 2 10 -2.373601891737092 2 11 -4.690378803740277 2 12 -4.240948156164617 2 13 -3.367918542311365 2 14 -1.9533688139858612 2 15 -3.6254306436900663 2 16 -3.9558431328249277 2 17 -2.866737020823898 2 18 -4.474734002804595 2 19 -2.177171265823141 2 20 -2.177268154658435 2 21 -4.121190868691965 2 22 -3.8003292943971694 2 23 -4.132407515027776 2 24 -2.1987853082878814 2 25 -4.787001807947124 2 26 -3.604769396735147 2 27 -3.7034655268430505 2 28 -3.4462868095920314 2 29 -5.656481504319526 2 30 -7.012557190058708 2 31 -7.577228493893102 2 32 -6.310120576047268 2 33 -6.7665217808673255 2 34 -6.606933921894122 2 35 -7.125695795252142 2 36 -5.116399730452276 2 37 -3.74133939343632 2 38 -5.106569553893422 2 39 -3.757909839984407 2 40 -3.948492891386781 2 41 -3.997988650380872 2 42 -3.19550390425427 2 43 -2.635533436097017 2 44 -2.8286920340891726 2 45 -3.633234065274472 2 46 -2.0995877251360184 2 47 -2.6134012531741426 2 48 -2.4304775752124606 2 49 -6.100056675264149 2 50 -3.7241598140335057 2 51 -4.786100126794317 2 52 -4.005080003199335 2 53 -4.0832607998636234 2 54 -2.211202047663985 2 55 -2.7482621540970236 2 56 -2.8739336197937284 3 1 -0.32351876157161286 3 2 -1.2173875512302672 3 3 -0.3622391831298586 3 4 0.1356325652505106 3 5 -0.4920312678969836 3 6 -0.8844331259100997 3 7 0.3277293640976424 3 8 -0.6201788567946167 3 9 -0.46840771302422723 3 10 0.013395012287674035 3 11 -0.3838772557526653 3 12 -0.3121555865051704 3 13 -0.1976399045404662 3 14 -0.5756598356576887 3 15 -1.3065451947736548 3 16 0.41876987027104917 3 17 0.2137583352132566 3 18 0.8039037680932353 3 19 0.4822714586350614 3 20 -0.8583827057420237 3 21 0.5320599053018427 3 22 -0.9489361055426784 3 23 0.6477782434861581 3 24 -0.29476054048602407 3 25 0.6402427740962224 3 26 1.5066857116239873 3 27 0.4327573833736693 3 28 1.2657874938135407 3 29 -0.5574102968891879 3 30 0.845027949260178 3 31 1.8407281624536438 3 32 1.75799087906109 3 33 0.5183079404949069 3 34 1.581920567269961 3 35 2.614242285243215 3 36 2.9950553779068585 3 37 2.766400544644174 3 38 3.5435852448681864 3 39 2.1708827296750393 3 40 2.7511975110900018 3 41 1.0020113496191279 3 42 0.6466013589036237 3 43 1.209667802293076 3 44 1.945301773960363 3 45 1.1036080399886856 3 46 2.5474710983933946 3 47 3.411366548683443 3 48 2.745993013022833 3 49 3.525330321639874 3 50 1.6194934367404876 3 51 1.987893681255219 3 52 2.9763441454074773 3 53 2.5854698502911724 3 54 2.8104697789614015 3 55 3.267217722063968 3 56 0.5316391356242303 4 1 0.48437597527048837 4 2 0.35103350140131795 4 3 -0.22802865681406814 4 4 -0.2459463821179071 4 5 -0.6999260047390834 4 6 0.33925108461621534 4 7 -0.7406916885339427 4 8 -1.064915698899874 4 9 -0.9157761340768573 4 10 -0.5971313035017971 4 11 -1.0786140978579226 4 12 -0.21741874439990738 4 13 -1.0502714834878333 4 14 -0.06513351986821903 4 15 0.003981121015819156 4 16 -1.7970196034131618 4 17 -0.6020311384709558 4 18 0.07232482072481616 4 19 -2.2624653834702 4 20 -0.7978563899525497 4 21 -0.315308515750786 4 22 -0.46209400027952086 4 23 -0.8206428091454256 4 24 -1.3105500141702393 4 25 -0.778178278535357 4 26 -0.9959458673233854 4 27 -0.8593478797842309 4 28 -2.1315809272390887 4 29 -1.1653050337312933 4 30 -3.465498366529296 4 31 -2.756640258598985 4 32 -3.663061752517855 4 33 -2.4106394279261494 4 34 -1.8628162748352999 4 35 -4.246284030546256 4 36 -2.607576201040512 4 37 -2.517809981671615 4 38 -1.9800989656581294 4 39 -2.4843804282197013 4 40 -1.9514340678573667 4 41 -3.3479886503808736 4 42 -3.0955039042542687 4 43 -2.6561216713911335 4 44 -4.025750857618586 4 45 -3.4332340652744726 4 46 -3.552528901606607 4 47 -3.328107135527084 4 48 -3.004006986977167 4 49 -2.9088802046759135 4 50 -2.4594539316805637 4 51 -2.383158950323729 4 52 -2.7315505914346296 4 53 -1.880319623393035 4 54 -3.305319694722808 4 55 -3.1012033305676105 4 56 -1.5209924433231397 5 1 0.3343759752704898 5 2 -0.40452205415424203 5 3 -0.7891397679251853 5 4 -0.7737241598956857 5 5 -0.8165926714057505 5 6 1.1448066401717725 5 7 -0.5406916885339399 5 8 0.31841763443345883 5 9 0.6397794214786998 5 10 1.2473131409426443 5 11 1.1658303465865174 5 12 1.5270257000445326 5 13 2.5830618498454996 5 14 1.8015331467984481 5 15 1.4039811210158177 5 16 0.9363137299201725 5 17 1.1201910837512656 5 18 1.7834359318359319 5 19 2.031979060974244 5 20 0.9632547211585631 5 21 1.1402470398047697 5 22 0.8990171108315934 5 23 1.6126905241879115 5 24 0.11167220805198497 5 25 0.10515505479797582 5 26 1.0096096882321746 5 27 0.25731878688243626 5 28 1.2628635172053535 5 29 0.2846949662687095 5 30 0.412279411248484 5 31 -1.1066402585989863 5 32 0.05916046970436639 5 33 0.7171383498516306 5 34 1.298294836275815 5 35 0.5148270805648565 5 36 1.9979793545150457 5 37 1.9710789072172723 5 38 1.1810121454529856 5 39 0.8989529051136316 5 40 -0.6347674011907003 5 41 2.613122460730242 5 42 2.80449609574573 5 43 3.0049894397199814 5 44 4.190915809048079 5 45 2.6445437125033067 5 46 2.9363599872822803 5 47 3.2330039755840296 5 48 2.0237707908006115 5 49 3.6744531286574222 5 50 2.0405460683194363 5 51 -0.7664922836570618 5 52 -1.2926617025457396 5 53 -0.8969862900597043 5 54 -0.3386530280561395 5 55 -1.4900922194564998 5 56 -0.7432146655453611 6 1 0.7788204197149327 6 2 0.8732557236235365 6 3 1.0441935654081504 6 4 1.504053617882093 6 5 1.3500739952609138 6 6 1.0336955290606582 6 7 1.2370860892438387 6 8 1.8184176344334588 6 9 1.4731127548120284 6 10 1.6362020298315372 6 11 2.7213859021420745 6 12 2.3603590333778683 6 13 3.1941729609566067 6 14 1.9681998134651195 6 15 1.6817588987935963 6 16 1.9363137299201725 6 17 1.5090799726401585 6 18 2.0056581540581533 6 19 2.86531239430758 6 20 0.12992138782522744 6 21 2.6402470398047697 6 22 2.5656837774982577 6 23 2.334912746410133 6 24 1.3894499858297635 6 25 2.8829328325757544 6 26 2.5096096882321746 6 27 1.0350965646602148 6 28 3.3184190727609106 6 29 2.8402505218242666 6 30 3.912279411248484 6 31 4.393359741401014 6 32 5.281382691926588 6 33 4.550471683184966 6 34 4.742739280720258 6 35 5.5703826361204065 6 36 6.497979354515046 6 37 6.915523351661722 6 38 6.736567701008536 6 39 6.398952905113635 6 40 7.5318992654759676 6 41 6.279789127396906 6 42 6.137829429079066 6 43 6.893878328608867 6 44 6.468693586825857 6 45 6.700099268058864 6 46 5.214137765060059 6 47 6.28855953113958 6 48 5.134881901911719 6 49 7.063342017546308 6 50 5.707212734986101 6 51 6.011285494120713 6 52 6.485116075232035 6 53 2.4363470432736314 6 54 3.4946803052771926 6 55 2.121018891654611 6 56 -1.6876591099898057 7 1 1.1501654489547022 7 2 0.8878756066644726 7 3 1.5851292379227715 7 4 1.2408957231452504 7 5 1.4553371531556536 7 6 0.1681984530372702 7 7 0.7487819956765875 7 8 1.2745579853106506 7 9 0.42632912908104004 7 10 1.592342380708729 7 11 1.2477016916157595 7 12 2.5825812556000898 7 13 2.0128864112490064 7 14 1.4243401643423113 7 15 2.0092442789105576 7 16 2.3661382913236793 7 17 1.0558635983711468 7 18 1.5407458733564 7 19 1.4296398796876986 7 20 1.9837225574158666 7 21 2.5846914842492126 7 22 1.5773796839310066 7 23 1.647778243486158 7 24 3.021028933198181 7 25 2.5349796162014826 7 26 2.4014225537292475 7 27 2.643283699163142 7 28 1.4763138096030133 7 29 3.074168650479237 7 30 3.897659528207548 7 31 2.6302018466641712 7 32 3.6000961422189874 7 33 4.307781624705434 7 34 4.160867935691016 7 35 4.403715969453742 7 36 3.310844851591071 7 37 4.029558439381017 7 38 4.701479981710289 7 39 4.276145887569772 7 40 4.540671195300529 7 41 5.844116612777025 7 42 5.4887066220615175 7 43 5.314930960187812 7 44 5.787407037118257 7 45 5.945713303146583 7 46 4.96852372997234 7 47 4.358734969736073 7 48 5.272308802496518 7 49 5.735856637429347 7 50 5.356335542003649 7 51 6.67210420757101 7 52 7.555291513828529 7 53 7.690733008185909 7 54 8.494680305277193 7 55 4.8461650904850195 7 56 4.847428609308439 8 1 -0.06036086683477038 8 2 0.1510335014013151 8 3 0.16407660634382637 8 4 0.34615888103999026 8 5 0.5606003110503863 8 6 -0.2002225995943121 8 7 0.43299252199237515 8 8 0.27455798531065057 8 9 0.42632912908104004 8 10 0.6976055386034616 8 11 2.2477016916157595 8 12 2.3194233608632473 8 13 2.9602548323016364 8 14 2.055919111710729 8 15 2.1145074368052903 8 16 1.8398225018499943 8 17 2.055863598371147 8 18 1.2249563996721875 8 19 1.5875346165298012 8 20 1.299512031100079 8 21 0.47942832635447274 8 22 1.893169157615219 8 23 1.0688308750651032 8 24 1.8631341963560786 8 25 -0.5176519627458873 8 26 0.2961593958345148 8 27 0.16959948863682683 8 28 0.2131559148661708 8 29 0.49522128205818206 8 30 0.31871215978649303 8 31 -0.26453499544108894 8 32 -0.5051670156757524 8 33 -1.008007848978778 8 34 0.055604777796283145 8 35 -0.12259982001994274 8 36 -3.3733656747247203 8 37 -2.9704415606189833 8 38 -2.666941070921286 8 39 -4.250169901903909 8 40 -3.196170909962632 8 41 -1.9979886503808721 8 42 -2.8270828516226914 8 43 -2.4219111450753417 8 44 -2.212592962881743 8 45 -2.264813012642893 8 46 -1.9788446910802904 8 47 -1.5360018723691908 8 48 -1.1487438290824308 8 49 -1.0536170467811772 8 50 -3.3805065632595124 8 51 -1.8015800029553084 8 52 -2.128919012487259 8 53 -1.8882143602351427 8 54 -1.2421617999859649 8 55 -1.2590980674097167 8 56 -0.36309770648103523 9 1 -0.11006846917395308 9 2 -1.9600776097097992 9 3 -1.9558064345918496 9 4 -1.1626130487845714 9 5 -1.8165926714057505 9 6 -0.6885266931615632 9 7 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3.289449985829762 114 25 1.7718217214646401 114 26 2.7540541326766146 114 27 2.640652120215769 114 28 2.968419072760909 114 29 2.684694966268708 114 30 2.284501633470704 114 31 2.1433597414010137 114 32 1.9369382474821464 114 33 1.3393605720738506 114 34 2.2871837251647023 114 35 2.653715969453742 114 36 3.6924237989594886 114 37 3.2821900183283894 114 38 3.8699010343418685 114 39 4.815619571780299 114 40 5.298565932142633 114 41 5.102011349619129 114 42 4.55449609574573 114 43 5.143878328608867 114 44 4.024249142381414 114 45 5.816765934725531 114 46 4.547471098393395 114 47 4.9218928644729125 114 48 5.945993013022836 114 49 6.191119795324088 114 50 4.490546068319439 114 51 4.666841049676272 114 52 5.618449408565368 114 53 5.869680376606965 114 54 6.744680305277193 114 55 8.048796669432388 114 56 3.279007556676861 115 1 0.05659819749271122 115 2 -0.1822998319320206 115 3 0.15530467651926472 115 4 -0.4959463821179071 115 5 -0.37214822696130767 115 6 -1.132971137606006 115 7 0.34819720035494583 115 8 -0.570471254455434 115 9 -0.804665022965743 115 10 -0.5860201923906843 115 11 1.1102747910309603 115 12 0.36035903337786834 115 13 0.5830618498454996 115 14 1.190422035687341 115 15 -0.26268554565084656 115 16 -0.6747973811909418 115 17 0.009079972640158473 115 18 -1.4387862903862967 115 19 -0.4124653834701988 115 20 -0.9256341677303297 115 21 1.4735803731380983 115 22 1.7879059997204791 115 23 1.334912746410133 115 24 1.167227763607542 115 25 0.4384883881313044 115 26 0.8429430215655032 115 27 0.25731878688243626 115 28 1.985085739427575 115 29 1.562472744046488 115 30 1.7456127445818197 115 31 1.7266930747343494 115 32 1.5036049141488093 115 33 2.3282494609627378 115 34 1.6871837251647008 115 35 2.681493747231521 115 36 2.2757571322928243 115 37 3.304412240550608 115 38 2.5699010343418713 115 39 3.898952905113635 115 40 2.9207881543648533 115 41 1.5020113496191279 115 42 2.6933849846346156 115 43 2.893878328608867 115 44 1.5242491423814144 115 45 3.5889881569477495 115 46 1.5474710983933946 115 47 2.288559531139583 115 48 1.4126596796895008 115 49 2.3411197953240865 115 50 2.4294349572083256 115 51 2.733507716342938 115 52 2.151782741898703 115 53 2.7141248210514064 115 54 3.383569194166082 115 55 -0.4345366639009427 115 56 -1.1876591099898057 116 1 0.36069176474417475 116 2 0.4668229750855275 116 3 0.5324976589754016 116 4 0.3987904599873531 116 5 0.8763897847345987 116 6 -0.252854178541682 116 7 0.43299252199237515 116 8 -0.04123148837356183 116 9 0.005276497502087807 116 10 1.1712897491297838 116 11 -0.4365088347000281 116 12 -0.5753134812420129 116 13 -0.7765872729615211 116 14 -1.417765098815586 116 15 -0.9907557210894424 116 16 1.6292961860605217 116 17 1.0558635983711468 116 18 0.8565353470406052 116 19 1.219113563898219 116 20 2.0889857153106064 116 21 -0.20478219996131486 116 22 1.4194849470888968 116 23 0.2793571908545758 116 24 0.8631341963560786 116 25 0.008663826727797641 116 26 -1.6512090252181224 116 27 -1.0935584061000156 116 28 -1.1026335588180345 116 29 -1.7153050337312905 116 30 -2.3128667875819247 116 31 -1.3697981533358288 116 32 -1.2946406998862763 116 33 -0.8501131121366754 116 34 -1.7338689064142478 116 35 -1.4383892937041516 116 36 -1.8470498852510389 116 37 -2.8651784027242435 116 38 -0.7722042288160225 116 39 -0.5659593755881218 116 40 -0.30143406785736815 116 41 -3.1032518082756084 116 42 -2.1428723253069 116 43 -1.0008585134963965 116 44 -3.686277173408058 116 45 -0.7384972231692082 116 46 -0.24200258581713285 116 47 0.8324191802623879 116 48 -0.4645333027666396 116 49 0.7884882163767166 116 50 0.5142302788457513 116 51 1.3036831549394279 116 52 1.7658178296180012 116 53 -0.3618985707614577 116 54 -2.1895302210385985 116 55 -0.890677014778138 116 56 -0.20520296963892903 117 1 0.5343759752704926 117 2 1.0510335014013137 117 3 0.2219713431859276 117 4 0.35405361788209433 117 5 0.8500739952609138 117 6 -1.1607489153837847 117 7 0.40930831146606295 117 8 -0.2649156988998769 117 9 0.2842238659231455 117 10 -0.04713130350179995 117 11 0.5213859021420717 117 12 1.4325812556000912 117 13 0.8497285165121653 117 14 1.0848664801317867 117 15 -1.1960188789841837 117 16 0.5529803965868396 117 17 0.6479688615290442 117 18 0.8223248207248162 117 19 0.6875346165298026 117 20 -0.047856389952549705 117 21 1.9846914842492112 117 22 1.0879059997204763 117 23 0.7293571908545786 117 24 2.489449985829765 117 25 0.5718217214646373 117 26 -0.9959458673233854 117 27 -2.5093478797842295 117 28 -2.23158092723909 117 29 -2.66530503373129 117 30 -2.815498366529294 117 31 -2.2066402585989877 117 32 -1.2130617525178558 117 33 -1.6606394279261494 117 34 -1.7628162748352985 117 35 -1.296284030546257 117 36 -1.45757620104051 117 37 -3.467809981671614 117 38 -4.130098965658128 117 39 -3.8843804282197 117 40 -6.201434067857367 117 41 -6.147988650380871 117 42 -5.595503904254269 117 43 -5.806121671391132 117 44 -5.825750857618587 117 45 -4.883234065274472 117 46 -4.902528901606605 117 47 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2.2507976668479586 119 12 2.4061106673647927 119 13 1.9850226341592219 119 14 0.5760429507200158 119 15 1.3745693563099337 119 16 1.4559215730574238 119 17 0.7803218027055152 119 18 0.40761893837187557 119 19 0.17576991064744618 119 20 1.528614198282746 119 21 0.8199856018962706 119 22 1.611435411485182 119 23 0.16171013203104678 119 24 -0.8458441318172945 119 25 1.977704074405814 119 26 1.1011129562060304 119 27 1.590652120215772 119 28 1.2595955433491426 119 29 2.1082243780334124 119 30 2.4580310452354084 119 31 1.5404185649304267 119 32 2.572232365129203 119 33 2.880537042662084 119 34 3.628360195752933 119 35 4.521363028277271 119 36 5.118894387194786 119 37 4.317484135975445 119 38 4.422842210812458 119 39 3.1244431011920675 119 40 4.698565932142632 119 41 5.237305467266186 119 42 5.157437272216317 119 43 5.187995975667693 119 44 4.81836678944024 119 45 5.543236522960825 119 46 5.194529921922808 119 47 6.03365757035527 119 48 6.216581248316949 119 49 5.899943324735855 119 50 6.74642842126061 119 51 6.56684104967627 119 52 6.818449408565371 119 53 3.3873274354304925 119 54 2.671150893512486 119 55 0.48703196355003797 119 56 0.5966546155003893 120 1 -0.3084811675866561 120 2 -0.7775379271701155 120 3 -1.2494572282426404 120 4 -2.353089239260761 120 5 -2.8642117190248015 120 6 2.2321082274733612 120 7 0.1735940257517754 120 8 0.12794144395726903 120 9 -0.05863327693400322 120 10 0.5171544107839168 120 11 2.792814473570644 120 12 2.3682955413143745 120 13 3.0354428022264486 120 14 2.134866480131784 120 15 0.8325525495872483 120 16 2.0315518251582674 120 17 1.6122545758147595 120 18 1.6008962492962482 120 19 2.373248902244086 120 20 0.6378578957617336 120 21 0.15612005567778198 120 22 2.0021917140061944 120 23 2.13650004799743 120 24 3.0323071286869094 120 25 1.8432502928932095 120 26 3.0969112755337633 120 27 0.5192235487872026 120 28 2.6755619299037647 120 29 2.6418378234115636 120 30 3.563073062042136 120 31 4.393359741401014 120 32 1.9083668189107144 120 33 1.7250748577881367 120 34 3.2586122965932702 120 35 2.4751445408823116 120 36 1.2281380846737733 120 37 -0.3463814102430405 120 38 2.498472462913302 120 39 2.49419100035173 120 40 2.198565932142632 120 41 3.859154206761982 120 42 2.947353238602876 120 43 2.536735471466013 120 44 2.952820570952845 120 45 3.652480220439813 120 46 2.976042526964825 120 47 4.264750007330061 120 48 2.67456444159426 120 49 3.1268340810383712 120 50 2.897688925462294 120 51 3.852555335390555 120 52 4.818449408565371 120 53 5.698251805178394 120 54 6.494680305277193 120 55 5.32736809800382 120 56 1.4790075566768603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40.0 3 6.0 4 25.0 5 27.0 6 23.0 7 66.0 8 42.0 9 42.0 10 64.0 11 62.0 12 87.0 13 115.0 14 83.0 15 117.0 16 119.0 17 171.0 18 180.0 19 214.0 20 295.0 21 257.0 22 280.0 23 372.0 24 574.0 25 468.0 26 228.0 27 267.0 28 347.0 29 445.0 30 581.0 31 701.0 32 934.0 33 1166.0 34 1395.0 35 1768.0 36 2595.0 37 4028.0 38 2709.0 39 503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 44.139091418956816 39.245653393157596 7.08543653019256 9.529818657693026 11 37.52103196859226 35.25425313142643 15.17106001121705 12.053654888764255 12 56.09459712095719 17.330342120022436 13.689474668162275 12.885586090858103 13 19.555057020003737 31.08057580856235 21.719012899607403 27.645354271826513 14 18.81192746307721 45.877734155917 20.56926528323051 14.741073097775285 15 32.4733595064498 19.6578799775659 34.520471116096466 13.34828939988783 16 16.933071602168628 19.218545522527574 45.09254066180595 18.75584221349785 17 27.930454290521595 17.923911011404 19.952327537857542 34.19330716021686 18 29.556926528323054 21.167507945410357 19.681248831557298 29.594316694709295 19 18.64834548513741 19.050289773789494 44.60646849878482 17.694896242288277 20 21.265657132174237 29.74387736025425 30.547765937558424 18.442699570013087 21 22.40605720695457 20.031781641428303 31.20676762011591 26.355393531501214 22 30.038324920545893 22.836044120396338 18.148252009721443 28.977378949336323 23 29.398018321181528 37.90428117405122 17.9706487193868 14.727051785380446 24 19.213871751729293 38.96055337446251 24.96260983361376 16.862965040194428 25 16.45238206554771 29.164523820655475 27.098041049137407 27.285053064659404 26 27.547346270750527 26.724339490296938 23.8812251578209 21.847089081131635 27 18.459091546989757 20.030874304158676 30.19132712728634 31.318707021565235 28 20.35000701885733 35.651115998315476 28.730522670909174 15.26835431191802 29 24.497916764196432 22.414680960629184 37.92893591124011 15.158466363934272 30 11.771867390897171 31.906724105637764 44.95692077168009 11.364487731784978 31 22.915886099662792 21.946421880854253 44.05207943049832 11.085612588984638 32 16.96805022018177 20.701770823573504 50.29982198069896 12.03035697554577 33 24.936720727477265 15.31827130402175 36.71604012374613 23.028967844754852 34 17.691730733170825 14.99624976561035 44.08400525032815 23.22801425089068 35 15.184056271981241 23.821805392731534 47.04806565064478 13.946072684642438 36 19.228603603603602 20.279654654654657 39.52233483483483 20.969406906906908 37 12.664820984468115 15.236262962789171 51.39130026746751 20.70761578527521 38 14.525821596244132 14.211267605633804 49.051643192488264 22.211267605633804 39 15.63130957411842 14.983330985584825 44.98755693290135 24.397802507395408 40 18.392370572207085 18.702433524382222 48.97585267311848 13.92934323029221 41 15.90076120665351 16.384738276477776 58.34977915609435 9.364721360774363 42 19.766730941071344 14.870902506701784 51.0840427032874 14.278323848939472 43 22.81468942493064 14.651807965392392 45.76574034889735 16.767762260779612 44 19.299153339604892 16.161806208842897 53.28316086547507 11.25587958607714 45 23.304626100051767 21.506894442091394 45.37154689632453 9.816932561532306 46 19.0238621923095 23.622158422365512 43.60144961641644 13.752529768908554 47 16.479118602570743 24.177221149771647 43.27416545035077 16.06949479730684 48 19.144526097606935 22.34313171283211 46.1984171848502 12.31392500471076 49 15.232131085789621 22.45032488934928 46.821734626612674 15.495809398248422 50 12.783796514366463 23.54686764013189 47.37164390014131 16.29769194536034 51 19.493241652145247 20.849620873169123 45.13257664955494 14.524560825130692 52 20.65586128910667 23.600640784018093 45.57576328684508 10.167734640030154 53 12.746206766563 26.07671284516068 49.6607294317218 11.516350956554518 54 12.995522036295073 27.83407966061749 45.1567287296724 14.013669573415036 55 22.952210387406918 21.740974644170045 43.99566405881799 11.311150909605052 56 19.888773682722217 28.35328494674333 41.58261853143557 10.175322839098879 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 9.0 13 21.5 14 25.0 15 28.5 16 32.0 17 46.0 18 60.0 19 130.0 20 200.0 21 291.5 22 318.5 23 254.0 24 334.0 25 414.0 26 484.0 27 554.0 28 712.0 29 968.0 30 1066.0 31 1141.5 32 1217.0 33 1235.0 34 1253.0 35 996.0 36 739.0 37 1128.5 38 1007.5 39 497.0 40 450.0 41 403.0 42 403.0 43 403.0 44 383.5 45 364.0 46 490.5 47 998.5 48 1380.0 49 1052.5 50 725.0 51 624.0 52 523.0 53 715.5 54 1465.0 55 2022.0 56 1630.0 57 1238.0 58 1081.0 59 924.0 60 855.5 61 787.0 62 700.0 63 537.5 64 462.0 65 381.5 66 301.0 67 244.0 68 187.0 69 155.0 70 123.0 71 99.0 72 56.0 73 37.0 74 29.5 75 22.0 76 33.0 77 44.0 78 47.5 79 60.0 80 69.0 81 40.0 82 11.0 83 8.0 84 5.0 85 3.0 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03271639558796036 26 0.05141147878108057 27 0.08880164516732099 28 0.1168442699570013 29 0.16358197793980184 30 0.1869508319312021 31 0.2056459151243223 32 0.2336885399140026 33 0.28977378949336324 34 0.29912133108992334 35 0.33183772667788375 36 0.3925967470555244 37 0.39727051785380446 38 0.448681996634885 39 0.4627033090297251 40 0.5141147878108057 41 0.532809871003926 42 0.6216115161712469 43 0.6029164329781267 44 0.6356328285660872 45 0.6870443073471677 46 0.6963918489437279 47 0.7337820153299682 48 0.7851934941110488 49 0.7384557861282482 50 0.7758459525144887 51 0.7618246401196486 52 0.8038885773041691 53 0.8132361189007292 54 0.8459525144886895 55 0.8319312020938493 56 0.8319312020938493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 21396.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.31725556178725 #Duplication Level Percentage of deduplicated Percentage of total 1 74.20979849861715 35.11404000747803 2 11.823389964440931 11.189007291082445 3 5.11655472145397 7.263039820527202 4 2.8447254049782695 5.38418395961862 5 1.72856578427499 4.089549448495046 6 1.155669695772422 3.280987100392597 7 0.75069142631371 2.4864460646849875 8 0.5630185697352825 2.131239484015704 9 0.38522323192414065 1.6404935501962985 >10 1.3137099960489924 8.96896616189942 >50 0.019755037534571314 0.6496541409609272 >100 0.05926511260371395 4.884090484202655 >500 0.029632556301856974 12.918302486446064 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCGGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 989 4.622359319498972 No Hit TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 896 4.1876986352589265 No Hit TCGGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 879 4.108244531688166 No Hit TCGGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 203 0.9487754720508507 No Hit TCGGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 179 0.8366049728921293 No Hit TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 174 0.8132361189007292 No Hit TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 171 0.7992148065058889 No Hit TCGGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 167 0.7805197233127688 No Hit TCGGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 151 0.7057393905402879 No Hit TCGGGTGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 75 0.35053280987100394 No Hit TCGGGTGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG 64 0.29912133108992334 No Hit TCGGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 0.2336885399140026 No Hit TCGGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.1869508319312021 No Hit TCGGGTGGGCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGAC 34 0.1589082071415218 No Hit TCGGGTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 32 0.14956066554496167 No Hit TCGGGTGGGATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTT 28 0.13086558235184145 No Hit TCGGGTGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27 0.1261918115535614 No Hit TCGGGTGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGAT 26 0.12151804075528137 No Hit TCGGGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 25 0.1168442699570013 No Hit TCGGGTGGGGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCCCCACGAGGCGCC 25 0.1168442699570013 No Hit TCGGGTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 24 0.11217049915872125 Illumina Single End Adapter 2 (96% over 30bp) TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 23 0.10749672836044122 Illumina Single End Adapter 2 (95% over 23bp) TCGGGTGGGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGA 22 0.10282295756216114 No Hit TCGGGTGGGAAGCTGGTACAACGAGAGTCGATCTTTTTTTTTTTTTTTTTTTTTTT 22 0.10282295756216114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.009347541596560104 0.0 0.0 0.0 0.0 11 0.056085249579360626 0.0 0.0 0.0 0.0 12 0.056085249579360626 0.0 0.0 0.0 0.0 13 0.056085249579360626 0.0 0.0 0.0 0.0 14 0.08412787436904094 0.0 0.0 0.0 0.0 15 0.08880164516732099 0.0 0.0 0.0 0.0 16 0.08880164516732099 0.0 0.0 0.0 0.0 17 0.08880164516732099 0.0 0.0 0.0 0.0 18 0.09347541596560105 0.0 0.0 0.0 0.0 19 0.0981491867638811 0.0 0.0 0.0 0.0 20 0.11217049915872125 0.0 0.0 0.0 0.0 21 0.11684426995700131 0.0 0.0 0.0 0.0 22 0.11684426995700131 0.0 0.0 0.0 0.0 23 0.13086558235184145 0.0 0.0 0.0 0.0 24 0.13086558235184145 0.0 0.0 0.0 0.0 25 0.13086558235184145 0.0 0.0 0.0 0.0 26 0.13086558235184145 0.0 0.0 0.0 0.0 27 0.14021312394840157 0.0 0.0 0.0 0.0 28 0.2149934567208824 0.0 0.0 0.0 0.0 29 0.21966722751916246 0.0 0.0 0.0 0.0 30 0.22901476911572258 0.0 0.0 0.0 0.0 31 0.23836231071228267 0.0 0.0 0.0 0.0 32 0.24303608151056272 0.0 0.0 0.0 0.0 33 0.24303608151056272 0.0 0.0 0.0 0.0 34 0.24303608151056272 0.0 0.0 0.0 0.0 35 0.29912133108992334 0.0 0.0 0.0 0.0 36 0.3131426434847635 0.0 0.0 0.0 0.0 37 0.3131426434847635 0.0 0.0 0.0 0.0 38 0.3178164142830436 0.0 0.0 0.0 0.0 39 0.3178164142830436 0.0 0.0 0.0 0.0 40 0.3178164142830436 0.0 0.0 0.0 0.0 41 0.3224901850813236 0.0 0.0 0.0 0.0 42 0.3318377266778837 0.0 0.0 0.0 0.0 43 0.34118526827444384 0.0 0.0 0.0 0.0 44 0.34118526827444384 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGCCG 20 4.0243706E-4 50.16706 50 TGCATTA 15 0.008209354 50.16706 40 TTACCGT 15 0.008209354 50.16706 44 CCTGCAT 15 0.008209354 50.16706 38 ACCGTCG 15 0.008209354 50.16706 46 CCGTCGG 15 0.008209354 50.16706 47 CATTACC 15 0.008209354 50.16706 42 TACCGTC 15 0.008209354 50.16706 45 CGTCGGC 15 0.008209354 50.16706 48 GGAGGCC 20 4.0243706E-4 50.16706 49 CCGGGGG 15 0.008209354 50.16706 39 GCATTAC 15 0.008209354 50.16706 41 AAGCAGG 15 0.008209354 50.16706 50 GTCTGGC 15 0.008286237 50.049297 37 TGCCTGC 15 0.008286237 50.049297 36 GCCTGCA 15 0.008286237 50.049297 37 TTGCCTG 15 0.008286237 50.049297 35 CGTTTGC 15 0.008363654 49.932087 32 GGATGGT 15 0.008363654 49.932087 8 GAGTGCG 30 1.0093445E-6 49.932087 29 >>END_MODULE