FastQCFastQC Report
Tue 31 May 2016
SRR212702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19495
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA10235.247499358809951No Hit
TCGGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA7824.011284944857656No Hit
TCGGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7683.939471659399846No Hit
TCGGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1840.943831751731213No Hit
TCGGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1780.9130546293921519No Hit
TCGGGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.8053347012054374No Hit
TCGGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1570.8053347012054374No Hit
TCGGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1450.7437804565273147No Hit
TCGGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.7386509361374711No Hit
TCGGGTGGGGGGAATTCCTTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG860.4411387535265453No Hit
TCGGGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.27186458066170816No Hit
TCGGGTGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG500.25647601949217746No Hit
TCGGGTGGGCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGAC400.20518081559374196No Hit
TCGGGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.16927417286483715No Hit
TCGGGTGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGAT290.14875609130546294No Hit
TCGGGTGGGAAGCTGGTACAACGAGAGTCGATCTTTTTTTTTTTTTTTTTTTTTTT280.1436265709156194No Hit
TCGGGTGGGATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTT250.12823800974608873No Hit
TCGGGTGGGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGA230.11797896896640163No Hit
TCGGGTGGGGGGCTGTTCGGTCTGGATAAAGAGATGAAACCGAAAAACGTGCTGGG210.10771992818671454No Hit
TCGGGTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG200.10259040779687098No Hit
TCGGGTGGGGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCCCCACGAGGCGCC200.10259040779687098No Hit
TCGGGTGGGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCCCCACGAGGCGCCG200.10259040779687098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTGT150.00817936250.19638449
GGGAGGG150.00826354450.06701343
CTGGCGG150.00826354450.06701339
GGAGGGC150.00826354450.06701344
TGGCGGC150.00826354450.06701340
CCTGGCG150.00826354450.06701338
GGCGGCC150.00826354450.06701341
GTGGGAC650.049.938315
AACGCGA150.0083483749.93830523
GGCATGC150.0083483749.9383058
ACGCAGG150.0083483749.93830524
GAAGTGT150.0083483749.9383059
GAAGCAT252.0267988E-549.9383059
GAACGCA150.0083483749.9383059
GGGAGAT252.0267988E-549.9383057
GAGCAGT252.0267988E-549.9383059
TGGGGTT252.0267988E-549.9383056
TGGGGTC252.0267988E-549.9383056
TGGGGCA252.0267988E-549.9383056
GTGGGTG451.2369128E-1049.9383055
Produced by FastQC (version 0.11.5)