SRR212701_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212701_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89646
Sequences flagged as poor quality	0
Sequence length	58
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	1656	1.847265912589519	No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	970	1.0820337773018318	No Hit
TAGCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	770	0.8589340294045468	No Hit
TAGCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	694	0.7741561252035786	No Hit
TAGCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	691	0.7708096289851193	No Hit
TAGCATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	685	0.7641166365482007	No Hit
TAGCATGGGGGGGGCTGGAGAGATGGCTCTGCAGTTAAGAACACCGGTTGCTCTTCCA	524	0.5845213394908864	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	512	0.5711353546170493	No Hit
TAGCATGGGATTTTCAAAATCTAATATTTTAATGTCAAAGAGTTTAATAAATGCTTTA	503	0.5610958659616715	No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	420	0.4685094705842982	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	418	0.4662784731053254	No Hit
TAGCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	385	0.4294670147022734	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	358	0.3993485487361399	No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	354	0.39488655377819426	No Hit
TAGCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	345	0.3848470651228164	No Hit
TAGCATGGGATGCAACTTAAGTGGAACCAAAAAAAAATTCAAAGATGATTTTGCTTGG	328	0.3658835865515472	No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGTTTTCGCTACCGTAGCGCAGGCCGCTCC	313	0.3491511054592508	No Hit
TAGCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	312	0.3480356067197644	No Hit
TAGCATGGGTGCTTAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG	304	0.33911161680387303	No Hit
TAGCATGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	289	0.3223791357115766	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	278	0.310108649577226	No Hit
TAGCATGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG	265	0.29560716596390246	No Hit
TAGCATGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA	265	0.29560716596390246	No Hit
TAGCATGGGACTTACATAGAAGATAATTGAAATTTTTTTCATATAGATATTATGACAA	238	0.26548869999776903	No Hit
TAGCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	237	0.26437320125828256	No Hit
TAGCATGGGGTCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	232	0.25879570756085046	No Hit
TAGCATGGGCGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	215	0.23983222898958123	No Hit
TAGCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	214	0.23871673025009482	No Hit
TAGCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	208	0.23202373781317626	No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	204	0.2275617428552306	No Hit
TAGCATGGGAGCGTGTTGTAGGATGACTTGGTCCCATTGGAAATCACTCGCACCACAA	200	0.22309974789728484	No Hit
TAGCATGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	183	0.20413626932601564	No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	181	0.2019052718470428	No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC	170	0.18963478571269213	No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC	164	0.1829417932757736	No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC	158	0.17624880083885505	No Hit
TAGCATGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC	153	0.17067130714142292	No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	152	0.16955580840193651	No Hit
TAGCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	150	0.16732481092296367	No Hit
TAGCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	149	0.16620931218347723	No Hit
TAGCATGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT	146	0.16286281596501795	No Hit
TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.16286281596501795	No Hit
TAGCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	140	0.1561698235280994	No Hit
TAGCATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	135	0.15059232983066728	No Hit
TAGCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC	131	0.1461303348727216	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA	129	0.14389933739374874	No Hit
TAGCATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG	128	0.14278383865426234	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG	125	0.13943734243580305	No Hit
TAGCATGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC	123	0.1372063449568302	No Hit
TAGCATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.13497534747785733	No Hit
TAGCATGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT	115	0.1282823550409388	No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	113	0.12605135756196595	No Hit
TAGCATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC	111	0.12382036008299312	No Hit
TAGCATGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG	111	0.12382036008299312	No Hit
TAGCATGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG	109	0.12158936260402026	No Hit
TAGCATGGGGGTGGTTTTTCTCCTTTAAATACCCCCTTACTCAGCTACTCTGGGCGCC	104	0.11601186890658813	No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTGCAGA	103	0.1148963701671017	No Hit
TAGCATGGGGGCTTCCTCCTCGTGGCGGCCCGGGAGCTAGGAGCACCGGTCTGCGCCG	103	0.1148963701671017	No Hit
TAGCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	102	0.1137808714276153	No Hit
TAGCATGGGGGGACTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCTAG	102	0.1137808714276153	No Hit
TAGCATGGGAGGCAATAATATTTTCTGTGTAAGCTTTTAAAATTATTTTTGGGGATCA	98	0.10931887646966959	No Hit
TAGCATGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA	94	0.10485688151172388	No Hit
TAGCATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	91	0.10151038529326462	No Hit
TAGCATGGGGGCGCCACAGTCCTCTACCCCTGCGTGGTGTATGACCGTGGGCCCGAGA	90	0.1003948865537782	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGCG	15	0.007160995	52.068752	52
TTCATAG	20	3.4081846E-4	52.068752	52
ATGATTG	15	0.007160995	52.068752	51
AAACAAC	20	3.4081846E-4	52.068752	51
ACCACAA	25	1.6300566E-5	52.068752	52
CAACCCC	15	0.007160995	52.068752	52
GTTCATA	20	3.4081846E-4	52.068752	51
CATTTAG	15	0.007160995	52.068752	51
TCAGCCT	20	3.4081846E-4	52.068752	52
CCCCGCC	15	0.007160995	52.068752	51
CGAACGC	15	0.007160995	52.068752	51
GTCAGCC	20	3.4081846E-4	52.068752	51
TTACGGC	15	0.007160995	52.068752	52
ATCCCGC	25	1.6354637E-5	52.039665	43
ACGTCAT	20	3.4176093E-4	52.039665	50
AATCACT	25	1.6354637E-5	52.039665	42
CATCCCG	25	1.6354637E-5	52.039665	42
AGAATGT	25	1.6354637E-5	52.039665	45
CACTCGC	25	1.6354637E-5	52.039665	45
AAGAATG	25	1.6354637E-5	52.039665	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content