FastQCFastQC Report
Tue 31 May 2016
SRR212700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95201
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17011.786745937542673No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10791.1333914559721012No Hit
TAGCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7860.8256215796052563No Hit
TAGCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7590.7972605329775948No Hit
TAGCATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT7490.7867564416340165No Hit
TAGCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6570.6901188012730959No Hit
TAGCATGGGATTTTCAAAATCTAATATTTTAATGTCAAAGAGTTTAATAAATGCTTTA5640.5924307517778175No Hit
TAGCATGGGGGGGGCTGGAGAGATGGCTCTGCAGTTAAGAACACCGGTTGCTCTTCCA5550.582977069568597No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5290.5556664320752933No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4090.42961733595235346No Hit
TAGCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3940.413861198936986No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC3900.4096595623995546No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3720.39075219798111366No Hit
TAGCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3660.38444974317496666No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3520.369744015293957No Hit
TAGCATGGGATGCAACTTAAGTGGAACCAAAAAAAAATTCAAAGATGATTTTGCTTGG3490.3665927878908835No Hit
TAGCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3420.3592399239503787No Hit
TAGCATGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3130.32877805905400154No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3100.32562683165092804No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGTTTTCGCTACCGTAGCGCAGGCCGCTCC3070.32247560424785454No Hit
TAGCATGGGTGCTTAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG2990.3140723311729919No Hit
TAGCATGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG2820.2962153758889087No Hit
TAGCATGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA2600.2731063749330364No Hit
TAGCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2550.26785432926124725No Hit
TAGCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2490.26155187445510025No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.25209819224587976No Hit
TAGCATGGGACTTACATAGAAGATAATTGAAATTTTTTTCATATAGATATTATGACAA2380.2499973739771641No Hit
TAGCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2320.2436949191710171No Hit
TAGCATGGGGTCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2270.23844287349922794No Hit
TAGCATGGGCGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2190.23003960042436528No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1950.2048297811997773No Hit
TAGCATGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1820.1911744624531255No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1800.18907364418440983No Hit
TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.18277118937826284No Hit
TAGCATGGGAGCGTGTTGTAGGATGACTTGGTCCCATTGGAAATCACTCGCACCACAA1740.18277118937826284No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1650.17331750716904235No Hit
TAGCATGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC1570.16491423409417968No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1570.16491423409417968No Hit
TAGCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1530.16071259755674835No Hit
TAGCATGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1520.15966218842239052No Hit
TAGCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1510.1586117792880327No Hit
TAGCATGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT1500.15756137015367486No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1420.1491580970788122No Hit
TAGCATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.14495646054138087No Hit
TAGCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1370.14390605140702303No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1370.14390605140702303No Hit
TAGCATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1370.14390605140702303No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA1360.1428556422726652No Hit
TAGCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1280.13445236919780254No Hit
TAGCATGGGAGGCAATAATATTTTCTGTGTAAGCTTTTAAAATTATTTTTGGGGATCA1190.12499868698858205No Hit
TAGCATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1190.12499868698858205No Hit
TAGCATGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1140.11974664131679288No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCAGA1110.1165954139137194No Hit
TAGCATGGGGGGACTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCTAG1110.1165954139137194No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1100.11554500477936155No Hit
TAGCATGGGAGCCTTGGCACAGTGATTCTGAGAAAGGTAGCTGTGCTTGAGTTACGTA1100.11554500477936155No Hit
TAGCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1080.1134441865106459No Hit
TAGCATGGGACTGCTTTGAGAGTTACTCAGAAAGTGATGCTGACTGCCCCACCTGCCT1060.11134336824193024No Hit
TAGCATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG1020.10714173170449891No Hit
TAGCATGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT1010.10609132257014106No Hit
TAGCATGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT1000.10504091343578323No Hit
TAGCATGGGGGCACTAAGGAGGCAGAGGAAGGCGGATCTCTGAGTTTGAAGTCAGCCT1000.10504091343578323No Hit
TAGCATGGGGGCTTCCTCCTCGTGGCGGCCCGGGAGCTAGGAGCACCGGTCTGCGCCG990.10399050430142541No Hit
TAGCATGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA980.10294009516706758No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG970.10188968603270973No Hit
TAGCATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG960.10083927689835191No Hit
TAGCATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.10083927689835191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACAA203.3971222E-452.10637352
CCGCCCC401.8299033E-952.10637352
CTTGTGG150.0071419252.10637352
TGTCACT251.6289428E-552.0789549
ACTTTGT150.00715681452.0789549
AGGTCCT150.00715681452.0789550
AAGCTTG150.00715681452.0789549
AGTCAGC307.8346966E-752.0789550
TTTGTGG150.00715681452.0789551
AAGTTAG251.6289428E-552.0789549
AGTTAGA251.6289428E-552.0789550
CACCAGC353.803143E-852.05155648
TACTCTG353.803143E-852.05155646
TAGAAAG150.00717173152.0515545
GTTTGTA150.00717173152.0515542
GTTTGAA203.4148383E-452.0515544
TAAATGC459.094947E-1152.0515548
TCTAGAA150.00717173152.0515543
ACGTACT203.4148383E-452.0515541
TCAGCTA307.8632183E-752.0515541
Produced by FastQC (version 0.11.5)