FastQCFastQC Report
Tue 31 May 2016
SRR212699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212699_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99771
Sequences flagged as poor quality0
Sequence length58
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17041.7079111164566858No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9310.9331368834631306No Hit
TAGCATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT7550.7567329183830973No Hit
TAGCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7460.7477122610778683No Hit
TAGCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7150.7166411081376352No Hit
TAGCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6260.627436830341482No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5850.5863427248398834No Hit
TAGCATGGGGGGGGCTGGAGAGATGGCTCTGCAGTTAAGAACACCGGTTGCTCTTCCA5240.5252027142155535No Hit
TAGCATGGGATTTTCAAAATCTAATATTTTAATGTCAAAGAGTTTAATAAATGCTTTA4600.46105581782281424No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4020.4029226929668942No Hit
TAGCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4020.4029226929668942No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3930.39390203566166526No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3830.38387908310029967No Hit
TAGCATGGGATGCAACTTAAGTGGAACCAAAAAAAAATTCAAAGATGATTTTGCTTGG3640.3648354732337052No Hit
TAGCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3640.3648354732337052No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC3580.3588217016968859No Hit
TAGCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3330.3337643202934721No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGTTTTCGCTACCGTAGCGCAGGCCGCTCC3310.33175972978119894No Hit
TAGCATGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3180.3187298914514238No Hit
TAGCATGGGTGCTTAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG3090.3097092341461948No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3060.30670234837778515No Hit
TAGCATGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA2780.2786380812059616No Hit
TAGCATGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG2710.27162201441300576No Hit
TAGCATGGGGTCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2640.2646059476200499No Hit
TAGCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2450.2455623377534554No Hit
TAGCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2350.23553938519208986No Hit
TAGCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2300.23052790891140712No Hit
TAGCATGGGACTTACATAGAAGATAATTGAAATTTTTTTCATATAGATATTATGACAA2290.22952561365527058No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.20847741327640296No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1960.19644987020276433No Hit
TAGCATGGGCGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1950.1954475749466278No Hit
TAGCATGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1820.1824177366168526No Hit
TAGCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1820.1824177366168526No Hit
TAGCATGGGAGCGTGTTGTAGGATGACTTGGTCCCATTGGAAATCACTCGCACCACAA1810.18141544136071602No Hit
TAGCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1760.17640396508003328No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1670.1673833077748043No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1470.14733740265207323No Hit
TAGCATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1460.1463351073959367No Hit
TAGCATGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1430.14332822162752704No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1380.13831674534684427No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1350.1353098595784346No Hit
TAGCATGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC1330.1333052690661615No Hit
TAGCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1280.12829379278547876No Hit
TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.12829379278547876No Hit
TAGCATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.12628920227320564No Hit
TAGCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1220.12228002124865943No Hit
TAGCATGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG1170.11726854496797666No Hit
TAGCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1160.11626624971184012No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1160.11626624971184012No Hit
TAGCATGGGGGCTTCCTCCTCGTGGCGGCCCGGGAGCTAGGAGCACCGGTCTGCGCCG1140.114261659199567No Hit
TAGCATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC1130.11325936394343046No Hit
TAGCATGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT1120.1122570686872939No Hit
TAGCATGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG1110.11125477343115736No Hit
TAGCATGGGAGGCAATAATATTTTCTGTGTAAGCTTTTAAAATTATTTTTGGGGATCA1100.11025247817502079No Hit
TAGCATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG1100.11025247817502079No Hit
TAGCATGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1100.11025247817502079No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA1090.10925018291888425No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCAGA1070.10724559240661113No Hit
TAGCATGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT1050.10524100189433805No Hit
TAGCATGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1040.10423870663820148No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1030.10323641138206494No Hit
TAGCATGGGGGTGGTTTTTCTCCTTTAAATACCCCCTTACTCAGCTACTCTGGGCGCC1030.10323641138206494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACA150.007025200452.3255847
GGGCAAT150.007025200452.3255847
GGGTATT150.007025200452.32558410
GGCAATT150.007025200452.3255848
GGAAGCG150.007025200452.3255848
TGGGCCA600.052.3255846
TGGGTAT150.007025200452.3255846
GGGAATG307.5866046E-752.3255847
GGAGTAA150.007025200452.3255849
GGATGGT251.5845038E-552.325588
GGCACTA203.3281246E-452.3255810
GTACCTG550.052.325589
ACGCATT203.3281246E-452.3255810
GATCGGT203.3281246E-452.325589
GAACAGT550.052.325589
TGGGCTC1900.052.325586
TGGGCAC251.5845038E-552.325586
TGGGTAC700.052.325586
GGGGTAG203.3281246E-452.325587
GGAACGC203.3281246E-452.325588
Produced by FastQC (version 0.11.5)