FastQCFastQC Report
Tue 31 May 2016
SRR212698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157299
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC31522.0038271063388833No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT30851.9612330656901826No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC15110.960590976420702No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12870.8181870196250453No Hit
TAGCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11650.7406277217274109No Hit
TAGCATGGGGGGGGCTGGAGAGATGGCTCTGCAGTTAAGAACACCGGTTGCTCTTC11430.7266416188278374No Hit
TAGCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11160.709476856178361No Hit
TAGCATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT9730.6185671873311337No Hit
TAGCATGGGATTTTCAAAATCTAATATTTTAATGTCAAAGAGTTTAATAAATGCTT9210.585509125932142No Hit
TAGCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA9200.5848733939821614No Hit
TAGCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC6920.43992650938658223No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT6690.4253046745370282No Hit
TAGCATGGGATGCAACTTAAGTGGAACCAAAAAAAAATTCAAAGATGATTTTGCTT6260.397968200687862No Hit
TAGCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5690.3617314795389672No Hit
TAGCATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA5590.3553741600391611No Hit
TAGCATGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT5000.3178659749903051No Hit
TAGCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4840.3076942637906153No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC4660.29625108869096434No Hit
TAGCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4610.29307242894106134No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4330.2752719343416042No Hit
TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.26891461484179807No Hit
TAGCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC4080.25937863559208896No Hit
TAGCATGGGTGCTTAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAG3920.24920692439239922No Hit
TAGCATGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3910.2485711924424186No Hit
TAGCATGGGCGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3730.2371280173427676No Hit
TAGCATGGGACTTACATAGAAGATAATTGAAATTTTTTTCATATAGATATTATGAC3720.236492285392787No Hit
TAGCATGGGATAGCATACCCAAAATAGTTTTTCACTAAGTTCTTTTGAGTATTCCT3690.23458508954284515No Hit
TAGCATGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3490.22187045054323293No Hit
TAGCATGGGGTCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3390.21551313104342684No Hit
TAGCATGGGAGCGTGTTGTAGGATGACTTGGTCCCATTGGAAATCACTCGCACCAC3380.21487739909344622No Hit
TAGCATGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTT3360.21360593519348503No Hit
TAGCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3190.20279849204381464No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGCATGGGAAGCAGT3080.19580544059402796No Hit
TAGCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2960.1881766571942606No Hit
TAGCATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.1805478737944933No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC2750.1748262862446678No Hit
TAGCATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2640.16783323479488108No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.16401884309499742No Hit
TAGCATGGGGGGTATTAGTTTCAAGCGGACCGCGCCGGCTTCAGGCTATTGGAAGG2550.1621116472450556No Hit
TAGCATGGGGGTTTTATCACATTGGAGCCTTACATGGTAGGAAAGGCGCTGACCTC2490.15829725554517193No Hit
TAGCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG2160.13731810119581178No Hit
TAGCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG2130.13541090534586997No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2120.13477517339588935No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC2050.1303250497460251No Hit
TAGCATGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT2020.12841785389608326No Hit
TAGCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1990.12651065804614142No Hit
TAGCATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.12587492609616083No Hit
TAGCATGGGGGCACTAAGGAGGCAGAGGAAGGCGGATCTCTGAGTTTGAAGTCAGC1870.11888187464637409No Hit
TAGCATGGGACTGCTTTGAGAGTTACTCAGAAAGTGATGCTGACTGCCCCACCTGC1820.11570321489647105No Hit
TAGCATGGGAGCCTTGGCACAGTGATTCTGAGAAAGGTAGCTGTGCTTGAGTTACG1770.11252455514656799No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCA1760.1118888231965874No Hit
TAGCATGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1740.11061735929662617No Hit
TAGCATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1700.10807443149670373No Hit
TAGCATGGGGGGACTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCT1690.10743869954672311No Hit
TAGCATGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC1660.1055315036967813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCA204.070282E-450.25248350
AGTCAGC252.0180843E-550.25248350
TTATGAC150.00825152350.25248350
GTAGGCC204.070282E-450.25248349
TTATATG150.00825152350.25248350
GCACCAC252.0180843E-550.25248350
TGACCTC451.2914825E-1050.25248350
CGATGAT150.00825152350.25248349
TAGGCCG204.070282E-450.25248350
TATTATG204.0767246E-450.2363948
TAAATGC800.050.2363948
TATCTCA150.00826198250.23638548
GCGATGA150.00826198250.23638548
CACCAAA150.00826198250.23638548
CAAGTGT204.0896342E-450.20422447
TGGAAGT204.0896342E-450.20422447
TTATCTC150.00828293150.20422447
TATTGGA150.00828293150.20422447
CTCGCAC252.0295947E-550.20422447
ATATTAT204.0896342E-450.20422447
Produced by FastQC (version 0.11.5)