FastQCFastQC Report
Tue 31 May 2016
SRR212697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212697_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149846
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC32372.1602178236322622No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT26371.7598067349145121No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC14500.9676601310678964No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12010.8014895292500301No Hit
TAGCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11850.7908119002175567No Hit
TAGCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11400.7607810685637254No Hit
TAGCATGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC11170.745431976829545No Hit
TAGCATGGGGGGGGCTGGAGAGATGGCTCTGCAGTTAAGAACACCGGTTGCTCTTC9760.6513353709808737No Hit
TAGCATGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT9470.6319821683595158No Hit
TAGCATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA9200.613963669367217No Hit
TAGCATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA7170.47849125101771156No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT6990.466478918356179No Hit
TAGCATGGGATTTTCAAAATCTAATATTTTAATGTCAAAGAGTTTAATAAATGCTT6740.4497951229929394No Hit
TAGCATGGGATGCAACTTAAGTGGAACCAAAAAAAAATTCAAAGATGATTTTGCTT5960.39774168145963185No Hit
TAGCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5380.35903527621691605No Hit
TAGCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4990.33300855545026226No Hit
TAGCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4820.32166357460325934No Hit
TAGCATGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT4800.32032887097420015No Hit
TAGCATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC4540.302977723796431No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4170.2782857066588364No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC4100.2736142439571293No Hit
TAGCATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA4010.2676080776263631No Hit
TAGCATGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT4000.2669407258118335No Hit
TAGCATGGGCGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3880.2589325040374785No Hit
TAGCATGGGTGCTTAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAG3550.23690989415800223No Hit
TAGCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.23157107964176551No Hit
TAGCATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3410.22756696875458807No Hit
TAGCATGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTT3380.2255649133109993No Hit
TAGCATGGGATAGCATACCCAAAATAGTTTTTCACTAAGTTCTTTTGAGTATTCCT3300.22022609879476263No Hit
TAGCATGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3240.21622198790758512No Hit
TAGCATGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3190.21288522883493718No Hit
TAGCATGGGAGCGTGTTGTAGGATGACTTGGTCCCATTGGAAATCACTCGCACCAC3190.21288522883493718No Hit
TAGCATGGGACTTACATAGAAGATAATTGAAATTTTTTTCATATAGATATTATGAC3150.21021582157681887No Hit
TAGCATGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3110.20754641431870052No Hit
TAGCATGGGGTCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3010.2008728961734047No Hit
TAGCATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.1962014334716976No Hit
TAGCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2720.18151969355204678No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC2540.16950736089051424No Hit
TAGCATGGGGGTTTTATCACATTGGAGCCTTACATGGTAGGAAAGGCGCTGACCTC2320.15482562097086341No Hit
TAGCATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG2170.14481534375291966No Hit
TAGCATGGGGGGTATTAGTTTCAAGCGGACCGCGCCGGCTTCAGGCTATTGGAAGG2150.1434806401238605No Hit
TAGCATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC2150.1434806401238605No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC2000.13347036290591674No Hit
TAGCATGGGGGCACTAAGGAGGCAGAGGAAGGCGGATCTCTGAGTTTGAAGTCAGC1850.123460085687973No Hit
TAGCATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA1830.12212538205891381No Hit
TAGCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1820.12145803024438423No Hit
TAGCATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.1181212711717363No Hit
TAGCATGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1760.11745391935720673No Hit
TAGCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGCATGGGAAGCAGT1730.11545186391361797No Hit
TAGCATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCA1730.11545186391361797No Hit
TAGCATGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1700.11344980847002922No Hit
TAGCATGGGACTGCTTTGAGAGTTACTCAGAAAGTGATGCTGACTGCCCCACCTGC1690.11278245665549964No Hit
TAGCATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT1650.11011304939738131No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1630.10877834576832213No Hit
TAGCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1610.10744364213926298No Hit
TAGCATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1570.10477423488114465No Hit
TAGCATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.10410688306661504No Hit
TAGCATGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1550.10343953125208546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACT600.050.36765749
GTATTAT150.00817637850.36765749
TTTAACA150.00817637850.36765750
GTCCTAA150.00817637850.36765749
TTATGAC309.903306E-750.36765750
ACCTCAA309.903306E-750.36765750
GATATGG150.00817637850.36765750
CAAGCCC251.9904432E-550.36765750
AGTTACG150.00817637850.36765750
TACTAAA150.00817637850.36765749
ATTATGA309.903306E-750.36765749
TTGGGAC150.00817637850.36765750
GTACCTC309.949563E-750.33367548
TGTATTA150.00819823150.33367548
TATTATG309.949563E-750.33367548
CTCGCAC309.949563E-750.33367547
CGTCCTA150.00819823150.33367548
CGATCCA150.00819823150.33367548
TATGTAA150.00819823150.33367547
ATATTAT309.949563E-750.33367547
Produced by FastQC (version 0.11.5)