SRR212696_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212696_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64175
Sequences flagged as poor quality	0
Sequence length	58
%GC	38

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	3779	5.888585897935333	No Hit
AAAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	1629	2.5383716400467473	No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	1283	1.9992208804051423	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1101	1.715621347876899	No Hit
AAAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	636	0.9910401246591353	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	606	0.9442929489676665	No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	541	0.8430074016361512	No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	377	0.5874561745227893	No Hit
AAAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	335	0.5220101285547332	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGTGG	298	0.46435527853525516	No Hit
AAAACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	262	0.4082586677054928	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	259	0.40358395013634596	No Hit
AAAACGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	237	0.36930268796260224	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	225	0.3506038176860148	Illumina Single End Adapter 2 (96% over 25bp)
AAAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	158	0.24620179197506817	No Hit
AAAACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	156	0.24308531359563693	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG	153	0.23841059602649006	No Hit
AAAACGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	147	0.2290611608881963	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG	143	0.22282820412933385	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	139	0.21659524737047137	Illumina Single End Adapter 2 (96% over 32bp)
AAAACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	135	0.21036229061160888	No Hit
AAAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.20257109466303075	No Hit
AAAACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.20257109466303075	No Hit
AAAACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG	122	0.1901051811453058	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGGGG	109	0.16984807167900273	No Hit
AAAACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT	103	0.160498636540709	No Hit
AAAACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102	0.15894039735099338	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC	98	0.1527074405921309	Illumina Single End Adapter 2 (100% over 31bp)
AAAACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC	97	0.15114920140241525	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT	93	0.1449162446435528	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA	92	0.14335800545383717	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC	88	0.1371250486949747	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCCG	85	0.13245033112582782	No Hit
AAAACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	83	0.12933385274639658	No Hit
AAAACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC	80	0.1246591351772497	No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA	71	0.11063498246980913	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT	68	0.10596026490066225	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCCG	67	0.10440202571094662	No Hit
AAAACGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67	0.10440202571094662	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG	66	0.10284378652123101	Illumina Single End Adapter 2 (96% over 30bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTTTT	20	3.4117236E-4	52.035133	34
GAAAACG	20	3.4117236E-4	52.035133	40
TGCGAAA	20	3.4117236E-4	52.035133	37
TGGGGTC	20	3.4117236E-4	52.035133	45
CAGTGCG	20	3.4117236E-4	52.035133	34
GTGCTTA	40	1.8353603E-9	52.035133	36
ATGGGGT	30	7.839626E-7	52.03513	44
TTGCAAT	15	0.007170043	52.03513	49
AGCTTTC	15	0.007170043	52.03513	34
AGCTCGA	25	1.631032E-5	52.03513	37
GGCCGTG	15	0.007170043	52.03513	50
CCCGGCA	15	0.007170043	52.03513	38
TATGCGT	30	7.839626E-7	52.03513	32
CACCGCC	15	0.007170043	52.03513	34
TCGAACT	65	0.0	52.03513	52
CGCTCCG	55	0.0	52.03513	52
ATGCGTT	30	7.839626E-7	52.03513	33
CCGTCTC	15	0.007170043	52.03513	41
ATGAAGG	15	0.007170043	52.03513	52
TCCAGTT	15	0.007170043	52.03513	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content