FastQCFastQC Report
Tue 31 May 2016
SRR212695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62988
Sequences flagged as poor quality0
Sequence length58
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT36895.856671111957834No Hit
AAAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG14952.373467962151521No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC12591.9987934209690736No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10761.7082618911538707No Hit
AAAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT6621.0509938400965262No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC4670.7414110624245889No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.7160093986156093No Hit
AAAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3410.5413729599288754No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.5143836921318345No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2460.3905505810630596No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC2220.35244808534959043Illumina Single End Adapter 2 (96% over 25bp)
AAAACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2160.34292246142122307No Hit
AAAACGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.3206960055883661No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGTGG2010.3191084016003048No Hit
AAAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.2651298660062234No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG1630.25877945005397857No Hit
AAAACGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1600.2540166380897949No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1480.2349653902330603No Hit
AAAACGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1360.21591414237632564Illumina Single End Adapter 2 (96% over 32bp)
AAAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.211151330412142No Hit
AAAACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.211151330412142No Hit
AAAACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1270.20162570648377467No Hit
AAAACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.18892487457928495No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC1110.17622404267479522Illumina Single End Adapter 2 (100% over 31bp)
AAAACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1030.16352321077030546No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC1020.16193560678224422No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC1020.16193560678224422No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGGGG940.1492347748777545No Hit
AAAACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.1412967549374484No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTT840.13335873499714232No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAAAA830.13177113100908108No Hit
AAAACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT810.12859592303295864No Hit
AAAACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.12859592303295864No Hit
AAAACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC770.12224550708071379No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCCG750.11907029910459135No Hit
AAAACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.11271988315234648No Hit
AAAACGGGGTTCTCTCTTCAATTACTGGTGAATTGTTAACAGGTAGATTTGTTTTTTT710.11271988315234648No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA680.10795707118816282No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.10795707118816282No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCCG670.1063694672001016No Hit
AAAACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA660.10478186321204039No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT630.10001905124785673No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTG630.10001905124785673Illumina Single End Adapter 2 (96% over 30bp)
AAAACGGGGAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT630.10001905124785673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT251.6274365E-552.0525539
AACCGCG251.6274365E-552.0525527
CGCCCCG503.6379788E-1252.0525552
TCGAATG251.6274365E-552.0525540
TGCGATT251.6274365E-552.0525536
GTAGATT150.0071599152.05254743
CTCGAAC150.0071599152.05254739
GAGGTGG150.0071599152.05254731
GGAGACA150.0071599152.05254752
AAACTGG150.0071599152.05254739
TTGCAAT203.4055783E-452.05254749
CAGTTCT150.0071599152.05254734
AGCTCGA401.8299033E-952.05254737
TATGCGT150.0071599152.05254732
TCGAACT800.052.05254752
CGCTCCG600.052.05254752
ATGCGTT150.0071599152.05254733
AGGGAGA150.0071599152.05254733
AGGTGGC150.0071599152.05254732
CAGAGGG850.052.05254727
Produced by FastQC (version 0.11.5)