FastQCFastQC Report
Tue 31 May 2016
SRR212694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64135
Sequences flagged as poor quality0
Sequence length58
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT33715.256100413190926No Hit
AAAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG13572.115849380213612No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC11311.7634676853512123No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9181.4313557339985967No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC6330.9869805878225618No Hit
AAAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT5880.9168160910579247No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.623684415685663No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGTGG3700.5769080845092384No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.46932252280346143No Hit
AAAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2860.44593435721524904No Hit
AAAACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2450.3820067046074686No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG2350.36641459421532707No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2180.33990800654868636No Hit
AAAACGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.2993685195291183No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1790.27909877601933425Illumina Single End Adapter 2 (96% over 25bp)
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1510.2354408669213378No Hit
AAAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.23076323380369534No Hit
AAAACGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1310.20425664613705463No Hit
AAAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.19490137990176973No Hit
AAAACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.18086848054884228No Hit
AAAACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1110.1730724253527715No Hit
AAAACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.16995400327434318No Hit
AAAACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1020.15903952599984408No Hit
AAAACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT1010.15748031496062992No Hit
AAAACGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1000.15592110392141575Illumina Single End Adapter 2 (96% over 32bp)
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGGGG850.1325329383332034No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTT820.12785530521556093No Hit
AAAACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.12473688313713262No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA680.10602635066656273No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCCG670.10446713962734856No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGC660.1029079285881344Illumina Single End Adapter 2 (100% over 31bp)
AAAACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATGGA650.10134871754892023No Hit
AAAACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.10134871754892023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATGT203.2753218E-452.4660388
AAGCGGT203.2753218E-452.46603810
GACTGGT203.2753218E-452.4660389
GGAACGC401.7062121E-952.4660388
GGAACAT401.7062121E-952.4660388
AAGCATA203.2753218E-452.46603810
AACATGG203.2753218E-452.46603810
ACAGTAT150.006939617552.46603410
GACAGTT150.006939617552.4660349
GGACTGT150.006939617552.4660348
GGACATT251.5531248E-552.4660348
GGCTGTC150.006939617552.4660348
GGACAGC251.5531248E-552.4660348
GGGAGAC150.006939617552.4660347
GGATACA150.006939617552.4660348
GGAAGTA150.006939617552.4660348
GAACATG251.5531248E-552.4660349
GACTGTA150.006939617552.4660349
GGGACTC150.006939617552.4660347
GGCTACG150.006939617552.4660348
Produced by FastQC (version 0.11.5)