SRR212693_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212693_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57717
Sequences flagged as poor quality	0
Sequence length	56
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	3745	6.488556231266351	No Hit
AAAACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	1198	2.0756449572916122	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1010	1.7499177018902574	No Hit
AAAACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	989	1.7135332744252127	No Hit
AAAACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	518	0.8974825441377757	No Hit
AAAACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	455	0.7883292617426408	No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.5977441654971672	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGT	269	0.4660671899093855	No Hit
AAAACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	230	0.3984961103314448	No Hit
AAAACGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	205	0.35518131573020084	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	190	0.3291924389694544	No Hit
AAAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	177	0.3066687457768075	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCC	162	0.2806798690160611	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	144	0.24949321690316545	Illumina Single End Adapter 2 (95% over 23bp)
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAACGGGGAAGCAGG	142	0.24602803333506593	No Hit
AAAACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	128	0.22177174835836927	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	124	0.21484138122217022	No Hit
AAAACGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	122	0.21137619765407073	No Hit
AAAACGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121	0.20964360587002098	No Hit
AAAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	116	0.2009806469497722	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	116	0.2009806469497722	Illumina Single End Adapter 2 (96% over 30bp)
AAAACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	113	0.1957828715976229	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCC	102	0.1767243619730755	No Hit
AAAACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.15939844413257792	No Hit
AAAACGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTC	84	0.14553770986017986	No Hit
AAAACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT	79	0.13687475093993104	No Hit
AAAACGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	78	0.1351421591558813	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA	77	0.13340956737183152	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	70	0.1212814248834832	Illumina Single End Adapter 2 (100% over 29bp)
AAAACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	66	0.11435105774728416	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT	65	0.11261846596323441	No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTT	65	0.11261846596323441	No Hit
AAAACGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.10915328239513489	No Hit
AAAACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	62	0.10742069061108511	No Hit
AAAACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.10568809882703536	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTGG	15	0.008142966	50.379826	48
GCCGCTC	20	3.9981422E-4	50.379826	50
TGGCGCT	15	0.008142966	50.379826	46
AGATTTG	15	0.0081712715	50.33567	45
GTAGATT	15	0.008199652	50.291595	43
GCCCAGA	15	0.008199652	50.291595	43
CGGTGGC	15	0.008199652	50.291595	43
GGTAGAT	15	0.008199652	50.291595	42
AGGTAGA	15	0.008199652	50.291595	41
TAGATTT	15	0.008199652	50.291595	44
CAGGTAG	15	0.008228105	50.247597	40
ACAGGTA	15	0.00825663	50.203674	39
AGCTAAA	20	4.0855666E-4	50.15983	37
AGAGCTA	20	4.0855666E-4	50.15983	35
GAGCGGA	30	1.0079366E-6	50.159824	36
GAGTGCT	15	0.00828523	50.159824	34
GCGAAAA	15	0.00828523	50.159824	38
TGTTAAC	15	0.00828523	50.159824	34
AGTGCTT	15	0.00828523	50.159824	35
TGCTTTA	15	0.00828523	50.159824	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content