FastQCFastQC Report
Tue 31 May 2016
SRR212692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40200
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8142.0248756218905473No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7871.9577114427860698No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6511.6194029850746268No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5681.4129353233830846No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3770.9378109452736318No Hit
CCGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC3520.8756218905472637No Hit
CCGACGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3020.7512437810945274No Hit
CCGACGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2900.7213930348258706No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2700.6716417910447761No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2510.6243781094527363No Hit
CCGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2360.5870646766169154No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.4800995024875622No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1860.46268656716417905No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1760.43781094527363185No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1670.41542288557213936No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1410.35074626865671643No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1250.3109452736318408No Hit
CCGACGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1250.3109452736318408No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1220.3034825870646766No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1080.26865671641791045No Hit
CCGACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1070.26616915422885573No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1030.2562189054726368No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1030.2562189054726368No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1020.2537313432835821No Hit
CCGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG990.24626865671641793No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC950.23631840796019898No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.2263681592039801No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG900.22388059701492538No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA890.22139303482587064No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC870.21641791044776118No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG860.2139303482587065No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA850.21144278606965175No Hit
CCGACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG840.208955223880597No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA840.208955223880597No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCGGGCCGCTCC830.2064676616915423No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA810.20149253731343283No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCGGGCCGCTCC810.20149253731343283No Hit
CCGACGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA800.1990049751243781No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC780.19402985074626866No Hit
CCGACGGGGGAGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA740.18407960199004975No Hit
CCGACGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC740.18407960199004975No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA720.1791044776119403No Hit
CCGACGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA720.1791044776119403No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC710.17661691542288557No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA710.17661691542288557No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCT700.17412935323383086No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA650.1616915422885572No Hit
CCGACGGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA630.15671641791044777No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA620.15422885572139303No Hit
CCGACGGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCAAA620.15422885572139303No Hit
CCGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC620.15422885572139303No Hit
CCGACGGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGGGC600.1492537313432836No Hit
CCGACGGGGATCACGCAGGAGGAGTATGGCGAATTCTATAAGAGCCTCACCAATGACT600.1492537313432836No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTCC560.13930348258706468No Hit
CCGACGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT560.13930348258706468No Hit
CCGACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA550.13681592039800997No Hit
CCGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG540.13432835820895522No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG530.13184079601990048No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCGGGCCGCCCC510.12686567164179105No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCT500.12437810945273632No Hit
CCGACGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAAG490.12189054726368159No Hit
CCGACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACGG490.12189054726368159No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA490.12189054726368159No Hit
CCGACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC480.11940298507462686No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT470.11691542288557215No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC470.11691542288557215No Hit
CCGACGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA460.11442786069651742No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG460.11442786069651742No Hit
CCGACGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC450.11194029850746269No Hit
CCGACGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA440.10945273631840796No Hit
CCGACGGGGGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAAAC440.10945273631840796No Hit
CCGACGGGGGTCTCAAGGCAACAAGGCAGAGGGCAATAGAGGAAGACACCTGATGTCA440.10945273631840796No Hit
CCGACGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC440.10945273631840796No Hit
CCGACGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT430.10696517412935325No Hit
CCGACGGGGCTCTCTCAGCTCCCACAGAGTAGCTAAACGTGCCAGAGCTGAGCCTCTG420.1044776119402985No Hit
CCGACGGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACATGCAGA420.1044776119402985No Hit
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC410.10199004975124377No Hit
CCGACGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA410.10199004975124377No Hit
CCGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT410.10199004975124377No Hit
CCGACGGGGAGCGTGCGTGGTGGAGAAGAGCTTTGGGACCCGTTCCTGCCAGCTCTGT410.10199004975124377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCGC850.052.0597849
CAGCCCA203.3884303E-452.05977652
TGCATCT150.007137262752.05977649
GTGCAGC203.3884303E-452.05977649
CAACCAA150.007137262752.05977649
GGCCGCT750.052.05977650
CCGCCCC503.6379788E-1252.05977652
CCGCTCC750.052.05977652
AACCAAA150.007137262752.05977650
TACAAAG251.6154954E-552.05977652
GATTTCC150.007137262752.05977651
GCCGCTC750.052.05977651
GCATCTT150.007137262752.05977650
AGATTTC150.007137262752.05977650
TGCAGCC203.3884303E-452.05977650
AGATTAA203.3884303E-452.05977649
GGTATGG150.007137262752.05977651
TGAGACG150.00717253851.99502617
GAGGCGT150.00717253851.99502610
ACGCTCG150.00717253851.99502617
Produced by FastQC (version 0.11.5)