FastQCFastQC Report
Tue 31 May 2016
SRR212691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42597
Sequences flagged as poor quality0
Sequence length58
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8171.9179754442801136No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7981.873371364180576No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6851.6080944667464843No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5261.2348287438082493No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4020.9437284315796888No Hit
CCGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC3470.8146113576073432No Hit
CCGACGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2920.6854942836349978No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2710.6361950372091931No Hit
CCGACGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2710.6361950372091931No Hit
CCGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2490.584548207620255No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2230.5235110453787826No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.4882972979317792No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1870.43899805150597454No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1830.4296077188534404No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1770.4155222198746391No Hit
CCGACGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1400.3286616428386976No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1360.31927131018616334No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1330.3122285606967627No Hit
CCGACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1310.3075333943704956No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1200.2817099795760265No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1180.27701481324975935No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1130.2652768974340916No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1130.2652768974340916No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.24180106580275607No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA980.2300631499870883No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC920.21597765100828695No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA910.2136300678451534No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC900.21128248468201988No Hit
CCGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG890.2089349015188863No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA880.20658731835575275No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG860.20189215202948566No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCT820.19250181937695143No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC820.19250181937695143No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA820.19250181937695143No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCGGGCCGCTCC810.19015423621381788No Hit
CCGACGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC810.19015423621381788No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCGGGCCGCTCC780.1831114867244172No Hit
CCGACGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA780.1831114867244172No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG770.18076390356128366No Hit
CCGACGGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCAAA750.17606873723501656No Hit
CCGACGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA750.17606873723501656No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA730.17137357090874944No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC730.17137357090874944No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA710.16667840458248231No Hit
CCGACGGGGGAGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA670.15728807192994812No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA650.152592905603681No Hit
CCGACGGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGGGC640.15024532244054747No Hit
CCGACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG620.14555015611428035No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCT620.14555015611428035No Hit
CCGACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA610.1432025729511468No Hit
CCGACGGGGATCACGCAGGAGGAGTATGGCGAATTCTATAAGAGCCTCACCAATGACT610.1432025729511468No Hit
CCGACGGGGCTCTCTCAGCTCCCACAGAGTAGCTAAACGTGCCAGAGCTGAGCCTCTG580.13615982346174615No Hit
CCGACGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC580.13615982346174615No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA580.13615982346174615No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT570.13381224029861258No Hit
CCGACGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAAG570.13381224029861258No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA560.13146465713547903No Hit
CCGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC560.13146465713547903No Hit
CCGACGGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA510.11972674131981126No Hit
CCGACGGGGGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAAAC510.11972674131981126No Hit
CCGACGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC510.11972674131981126No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCGGGCCGCCCC490.11503157499354415No Hit
CCGACGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT490.11503157499354415No Hit
CCGACGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT490.11503157499354415No Hit
CCGACGGGGGAGGCGTACGCTCGGTGGCTCCTCGGGTAAGATGGCGGCTGCATCTTTG490.11503157499354415No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA480.11268399183041058No Hit
CCGACGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA470.11033640866727704No Hit
CCGACGGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA470.11033640866727704No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG470.11033640866727704No Hit
CCGACGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTT460.10798882550414347No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC460.10798882550414347No Hit
CCGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG460.10798882550414347No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA460.10798882550414347No Hit
CCGACGGGGAATGCACATCTCTCCTAGTGACTCAGCCCTCAGCTCACTTCAGGCAGCT450.10564124234100994No Hit
CCGACGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATG430.10094607601474283No Hit
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC430.10094607601474283No Hit
CCGACGGGGCATCTGCAGTCTAGCAGAGATGGAAGAGCAGTCATTATTCCAAGGTTGC430.10094607601474283No Hit
CCGACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACGG430.10094607601474283No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG430.10094607601474283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCAA550.052.20331225
ACACGGT150.00706258752.2033124
CCAGTAG150.00706258752.2033126
GCTGGGT600.052.2033127
TTGGCGC150.00706258752.2033125
ACGGTGG150.00706258752.2033126
CAGCCCA150.00712895652.0801952
GTATGGG251.6133048E-552.0801952
TTGTATG251.6133048E-552.0801950
TCTGGTA150.00712895652.0801950
CCGCCCC353.720197E-852.0801952
CCGCTCC458.731149E-1152.0801952
TGCTGTA150.00712895652.0801952
TCGGAGA150.00712895652.0801951
ATTAAGC150.00712895652.0801951
AGTCGGA150.00712895652.0801949
ATATGGA251.6133048E-552.0801952
TGTATGG251.6133048E-552.0801951
AGATCCC650.052.0801922
CATCGAA203.3840514E-452.0801951
Produced by FastQC (version 0.11.5)