FastQCFastQC Report
Tue 31 May 2016
SRR212690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44547
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8001.7958560621366197No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7841.7599389408938872No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7231.6230049161559699No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5241.176285720699486No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3970.8911935708352976No Hit
CCGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC2970.66671156306822No Hit
CCGACGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2930.657732282757537No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2880.6465081823691831No Hit
CCGACGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2880.6465081823691831No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2490.5589601993400228No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2180.48937077693222886No Hit
CCGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2050.46018811592250886No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1940.4354950950681303No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.4310054549127887No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1910.42876063483511795No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1910.42876063483511795No Hit
CCGACGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1360.30529553056322534No Hit
CCGACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1350.30305071048555454No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1280.2873369699418592No Hit
CCGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG1100.24693020854378522No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1040.23346128807776056No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1020.228971647922419No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCGGGCCGCTCC990.22223718768940667No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA960.21550272745639434No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA950.21325790737872358No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.2110130873010528No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC940.2110130873010528No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTACTG930.20876826722338201No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA860.19305452667968662No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA850.19080970660201585No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC830.18632006644667432No Hit
CCGACGGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCAAA820.18407524636900352No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT820.18407524636900352No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG800.17958560621366199No Hit
CCGACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG750.1683615058253081No Hit
CCGACGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA750.1683615058253081No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA710.159382225514625No Hit
CCGACGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA700.15713740543695423No Hit
CCGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC690.15489258535928344No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCGGGCCGCTCC680.15264776528161267No Hit
CCGACGGGGGAGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA660.14815812512627113No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA660.14815812512627113No Hit
CCGACGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA650.14591330504860034No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC640.1436684849709296No Hit
CCGACGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC640.1436684849709296No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA630.1414236648932588No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA620.13917884481558804No Hit
CCGACGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC610.13693402473791727No Hit
CCGACGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT600.13468920466024648No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTCT540.12122028419422183No Hit
CCGACGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT530.11897546411655105No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA520.11673064403888028No Hit
CCGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG510.1144858239612095No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA510.1144858239612095No Hit
CCGACGGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA500.11224100388353873No Hit
CCGACGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC490.10999618380586795No Hit
CCGACGGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTT480.10775136372819717No Hit
CCGACGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG460.10326172357285565No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCT450.10101690349518487No Hit
CCGACGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAAG450.10101690349518487No Hit
CCGACGGGGATCACGCAGGAGGAGTATGGCGAATTCTATAAGAGCCTCACCAATGACT450.10101690349518487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTT203.306529E-452.3299378
CGGGGTA401.724402E-952.3299375
CGGGGAC1600.052.3299375
GATGTTA203.306529E-452.3299379
CCATCTC203.306529E-452.32993710
GAGTACA203.306529E-452.3299379
GGGGATA203.306529E-452.3299376
GGAGTAC203.306529E-452.3299378
CGGGGAT2150.052.3299335
GAAGCTG353.5868652E-852.3299339
GGACAGT353.5868652E-852.3299338
GGGCATC150.0069972252.329937
GGACTTT150.0069972252.329938
GGGTACC150.0069972252.329937
GAACGCA150.0069972252.329939
GGATCAC251.5693062E-552.329938
GGAAGTC150.0069972252.329938
GGGATTG150.0069972252.329937
GGGATTC150.0069972252.329937
GAACAGT150.0069972252.329939
Produced by FastQC (version 0.11.5)