FastQCFastQC Report
Tue 31 May 2016
SRR212689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67222
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT21513.1998452887447564No Hit
CCGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA13291.9770313290291868No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13051.9413287316652286No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC10981.6333938294010888No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9721.4459551932403083No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5760.8568623367349975No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5600.8330606051590254No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5550.825622564041534No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4800.7140519472791645No Hit
CCGACGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4450.6619856594567255No Hit
CCGACGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4050.6024813305167951No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.5028115795424117No Hit
CCGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3160.47008419862545No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2960.44033203415548483No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2370.3525631489690875No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1650.24545535687721282No Hit
CCGACGGGGGTCTCAAGGCAACAAGGCAGAGGGCAATAGAGGAAGACACCTGATGT1610.23950492398321976No Hit
CCGACGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1580.235042099312725No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC1570.23355449108922677No Hit
CCGACGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC1530.22760405819523372No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1520.22611644997173544No Hit
CCGACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1520.22611644997173544No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1420.21124036773675284No Hit
CCGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1350.200827110172265No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1330.1978518937252685No Hit
CCGACGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1240.1844634197137842No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC1150.17107494570229984No Hit
CCGACGGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCA1140.1695873374788016No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC1090.16214929636131029No Hit
CCGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.1576864716908155No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTAC1060.1576864716908155No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1050.15619886346731726No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1040.15471125524381898No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1020.1517360387968225No Hit
CCGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG1020.1517360387968225No Hit
CCGACGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCA990.14727321412632768No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC970.14429799767933116No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG970.14429799767933116No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT960.1428103894558329No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG930.13834756478533813No Hit
CCGACGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA930.13834756478533813No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC930.13834756478533813No Hit
CCGACGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT920.13685995656183989No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT920.13685995656183989No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC900.13388474011484336No Hit
CCGACGGGGGAGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC890.13239713189134508No Hit
CCGACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC870.12942191544434856No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG850.12644669899735206Illumina Single End Adapter 2 (95% over 23bp)
CCGACGGGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG830.12347148255035553No Hit
CCGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT810.12049626610335902No Hit
CCGACGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT760.11305822498586772No Hit
CCGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.11305822498586772No Hit
CCGACGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG750.11157061676236946No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA740.11008300853887121No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCGGGCCGCC740.11008300853887121No Hit
CCGACGGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG730.10859540031537296No Hit
CCGACGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG720.10710779209187468No Hit
CCGACGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAA720.10710779209187468No Hit
CCGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC710.10562018386837642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC150.00830776250.13463248
CCAACTG150.00830776250.13463244
CCGGAGC355.0808012E-850.13463240
GTCGCGT301.0140193E-650.13463244
TCGCGTG301.0140193E-650.13463245
TTATTGG150.00830776250.13463250
CGTAGCG451.2914825E-1050.13463241
TAGCAAG150.00830776250.13463248
TTCCTGC150.00830776250.13463243
ATTTGGT150.00830776250.13463247
CTAGCAA150.00830776250.13463247
CGTGCAG355.0808012E-850.13463248
TGGGTCA150.00830776250.13463243
AAGGAGG150.00830776250.13463246
GGGCGCC150.00830776250.13463250
TCCTGAG150.00830776250.13463246
ATTTCCT150.00830776250.13463243
TGGCTAG150.00830776250.13463244
CAGCTCT252.0336423E-550.1346350
GACACCT252.0336423E-550.1346345
Produced by FastQC (version 0.11.5)