FastQCFastQC Report
Tue 31 May 2016
SRR212688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67159
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18732.7889039443708215No Hit
CCGACGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA13952.0771601721288286No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13011.9371938236126207No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC12781.9029467383373786No Hit
CCGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA10291.5321848151401898No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6590.9812534433210739No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6410.9544513765839276No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5880.8755341800801083No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4510.6715406721362737No Hit
CCGACGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4430.6596286424753197No Hit
CCGACGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4310.641760597983889No Hit
CCGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3440.5122172754210158No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3130.46605816048481963No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.44074509745529267No Hit
CCGACGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC2770.41245402701052725No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.37076192319718876No Hit
CCGACGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2130.3171577897228964No Hit
CCGACGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG2110.314179782307658No Hit
CCGACGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1930.2873777155705118No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC1640.24419660804955406No Hit
CCGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1580.23526258580383866No Hit
CCGACGGGGGTCTCAAGGCAACAAGGCAGAGGGCAATAGAGGAAGACACCTGATGT1530.22781756726574248No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1410.20994952277431173No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC1410.20994952277431173No Hit
CCGACGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1400.20846051906669247No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1330.19803749311335786No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1220.1816584523295463No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTAC1210.18016944862192708No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1160.1727244300838309No Hit
CCGACGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1150.17123542637621167No Hit
CCGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG1140.16974642266859244No Hit
CCGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1130.1682574189609732No Hit
CCGACGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1110.16527941154573475No Hit
CCGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1110.16527941154573475No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC1080.16081240042287706No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC1030.15336738188478088No Hit
CCGACGGGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1010.15038937446954243No Hit
CCGACGGGGGGGCTGGTGAGACGGCTCAGTGGTTAAGAGCACTGACTACTCTTCCA1010.15038937446954243No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC990.14741136705430397No Hit
CCGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT980.14592236334668474No Hit
CCGACGGGGGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTGAGGAAA970.1444333596390655No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG970.1444333596390655No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT960.14294435593144628No Hit
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG950.14145535222382705No Hit
CCGACGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCGGGCCGCC950.14145535222382705No Hit
CCGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGC940.13996634851620782No Hit
CCGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.13996634851620782No Hit
CCGACGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT920.13698834110096933No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC890.13252132997811164No Hit
CCGACGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCA870.12954332256287318No Hit
CCGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.12805431885525395No Hit
CCGACGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG860.12805431885525395No Hit
CCGACGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT860.12805431885525395No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC800.11912029660953855No Hit
CCGACGGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT790.11763129290191933No Hit
CCGACGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG790.11763129290191933No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG780.11614228919430009Illumina Single End Adapter 2 (95% over 23bp)
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG760.11316428177906163No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT760.11316428177906163No Hit
CCGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC740.11018627436382317No Hit
CCGACGGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG740.11018627436382317No Hit
CCGACGGGGGAGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC710.10571926324096548No Hit
CCGACGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC710.10571926324096548No Hit
CCGACGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG690.10274125582572699No Hit
CCGACGGGGGGAGCAGCCTCTGCTGTGCTAACTGGCTAATAAAAACACAGTAGGCC690.10274125582572699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTT150.00824236850.23480646
GTCCGCG150.00824236850.23480649
CTCTTAC150.00824236850.23480650
CTGATGT252.0097055E-550.23480650
TCTACAC150.00824236850.23480646
TGCCCTC150.00824236850.23480647
ACCTGAT252.0097055E-550.23480648
CCTCCTG150.00824236850.23480650
TTCTTCG150.00824236850.23480650
CGTCCGC150.00824236850.23480648
CTTCTGA150.00824236850.23480650
TTCGCAC150.00826686250.1971544
CCCGGGT150.00826686250.1971544
TCGCACC150.00826686250.1971545
TGGCGTT150.00826686250.1971545
GACACCT252.0186566E-550.19714745
CGTTCGC150.00829140850.1595542
ACCGGAG650.050.1595539
ACCGTAG550.050.1595539
CGTAGCG550.050.1595541
Produced by FastQC (version 0.11.5)