FastQCFastQC Report
Tue 31 May 2016
SRR212686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312178
Sequences flagged as poor quality0
Sequence length58
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT94953.041533996630128No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA43101.3806225935203633No Hit
GAGATCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT29220.936004458994548No Hit
GAGATCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC26780.8578439223776179No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA20810.6666068717206209No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGTGG20760.6650052213801101No Hit
GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC19460.6233623125268276No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC15100.48369840283428045No Hit
GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG14320.458712657522311No Hit
GAGATCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT13460.4311642716655241No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10860.3478784539589593No Hit
GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC10110.3238536988512964No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.3158454471487421No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9210.2950239927221009No Hit
GAGATCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8620.2761245187040727No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA8440.27035857747823355No Hit
GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.26235032577567924No Hit
GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.2613893355713727No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.2306376490335642No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA6780.2171837861732729No Hit
GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5800.18579143949926005No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC5440.17425955704758184Illumina Single End Adapter 2 (96% over 25bp)
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGGGG5390.17265790670707096No Hit
GAGATCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5350.17137658643466228No Hit
GAGATCGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5230.1675326256174362No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCCAA4960.15888371377867755No Hit
GAGATCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4630.14831282153130587No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG4250.13614027894342332No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4190.13421829853481027No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.13325730833050375No Hit
GAGATCGGGAGCCATGTTGTTTGCCTTGCCTCAGAATTTACCTTTATGATTGGAAGAG4050.12973367758137985No Hit
GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3990.1278116971727668No Hit
GAGATCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT3980.12749136710466466No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.12653037690035812No Hit
GAGATCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA3920.1255693866960516No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.1255693866960516No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA3910.12524905662794944No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAA3850.1233270762193364No Hit
GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.1233270762193364No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA3710.118842455265906No Hit
GAGATCGGGGCCATTGCTTTCTGGATATGGATTAACAAACATCCTTGCAGAGATTAGA3350.10731057281422778No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGAGATCGGGAAGCAGTGG3270.10474793226941041No Hit
GAGATCGGGAGGAGCAGGAAGGCGTCATCTGTTAGAAAGAGGGAAAGGAACTCTTGAT3260.10442760220130823No Hit
GAGATCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3230.1034666119970017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTA203.3981245E-452.14069727
CCTCGAC150.007138533552.14069727
ACGCGAT459.094947E-1152.12393624
CTCGTGA150.00714761752.12393626
ACACCTC150.00714761752.12393624
CGCGATC503.6379788E-1252.12393625
ACCTCGA150.00714761752.12393626
ACGTGCC150.00714761752.12393626
GCTACTA203.403524E-452.12393624
CCACGTG150.00714761752.12393624
CCTAATC150.007152162552.1155622
TATGACA150.007152162552.1155621
AAATTGA251.630822E-552.1155623
TGCTACT203.4062265E-452.1155623
ATAACTC150.007152162552.1155622
GCCACGT150.007152162552.1155623
GCGTCAT401.8517312E-952.1155622
GAGGGAC150.007152162552.1155621
CTTAATA150.007179478652.0653552
GTCGAAC353.8329745E-852.0653552
Produced by FastQC (version 0.11.5)