##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 312178 Sequences flagged as poor quality 0 Sequence length 58 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.57433259230311 39.0 38.0 39.0 33.0 40.0 2 36.131155943083755 38.0 35.0 39.0 30.0 40.0 3 36.776387189359916 38.0 36.0 39.0 33.0 40.0 4 36.2912601144219 38.0 35.0 39.0 31.0 40.0 5 36.67978525072235 39.0 35.0 39.0 31.0 40.0 6 37.11965609363889 39.0 36.0 39.0 33.0 40.0 7 37.131123910076944 39.0 37.0 39.0 33.0 40.0 8 36.880372736067244 39.0 37.0 39.0 31.0 40.0 9 36.662413751129165 39.0 37.0 39.0 31.0 40.0 10 35.78779414308504 38.0 35.0 39.0 29.0 40.0 11 35.952527083907256 38.0 35.0 39.0 30.0 40.0 12 35.771928194811935 38.0 35.0 39.0 29.0 40.0 13 35.58229279449545 38.0 35.0 39.0 29.0 40.0 14 35.4410592674692 38.0 35.0 39.0 28.0 40.0 15 35.48028368430831 38.0 35.0 39.0 29.0 40.0 16 34.999359339863794 38.0 35.0 39.0 28.0 40.0 17 35.143171523938264 38.0 35.0 39.0 28.0 40.0 18 34.97639808058223 38.0 35.0 39.0 28.0 40.0 19 34.346392122442964 38.0 33.0 39.0 26.0 40.0 20 34.60652896744806 38.0 33.0 39.0 27.0 40.0 21 34.46227793118029 38.0 34.0 39.0 26.0 40.0 22 34.29969760841571 38.0 33.0 39.0 26.0 40.0 23 33.98986155334457 38.0 33.0 39.0 25.0 40.0 24 33.93435155584314 38.0 33.0 39.0 25.0 40.0 25 33.8481251081114 38.0 33.0 39.0 25.0 40.0 26 33.353823780022935 38.0 33.0 39.0 22.0 40.0 27 33.00889236269052 38.0 33.0 39.0 19.0 40.0 28 32.65744863507358 36.0 32.0 39.0 19.0 40.0 29 32.900620799671984 37.0 33.0 39.0 20.0 40.0 30 32.218718167199484 36.0 31.0 39.0 16.0 40.0 31 31.553325346436967 36.0 31.0 39.0 2.0 40.0 32 31.073102524841598 36.0 30.0 39.0 2.0 40.0 33 31.013059856876527 36.0 30.0 38.0 2.0 40.0 34 30.615482192851513 35.0 29.0 38.0 2.0 40.0 35 30.254909058293666 35.0 28.0 38.0 2.0 40.0 36 30.7828546534349 36.0 30.0 39.0 2.0 40.0 37 30.583119246071153 36.0 29.0 39.0 2.0 40.0 38 30.55124960759567 36.0 30.0 39.0 2.0 40.0 39 30.517470801914293 36.0 30.0 39.0 2.0 40.0 40 30.38910173042303 36.0 29.0 39.0 2.0 40.0 41 30.611606199027477 36.0 30.0 39.0 2.0 40.0 42 30.439637001966826 36.0 30.0 39.0 2.0 40.0 43 30.29699402264093 36.0 29.0 39.0 2.0 40.0 44 30.200321611388375 36.0 29.0 39.0 2.0 40.0 45 29.896770432253394 36.0 29.0 39.0 2.0 40.0 46 29.475328178154772 36.0 28.0 39.0 2.0 40.0 47 29.430405089404122 36.0 28.0 39.0 2.0 40.0 48 29.33900531107253 35.0 27.0 39.0 2.0 40.0 49 29.145734164483084 35.0 27.0 39.0 2.0 40.0 50 28.90945229964956 35.0 26.0 39.0 2.0 40.0 51 28.592754774519666 35.0 26.0 39.0 2.0 40.0 52 28.429517775115478 35.0 25.0 39.0 2.0 40.0 53 28.22040950995906 35.0 24.0 39.0 2.0 40.0 54 28.013149549295594 35.0 23.0 39.0 2.0 40.0 55 27.77392064783553 35.0 21.0 39.0 2.0 40.0 56 27.240603117452224 35.0 2.0 39.0 2.0 40.0 57 26.856802849656287 35.0 2.0 39.0 2.0 39.0 58 26.461787826176092 35.0 2.0 39.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.0760842017088166 1 2 -2.3067185632270295 1 3 -1.9535002356751079 1 4 -2.9256525217402114 1 5 -2.808338745444374 1 6 -2.563822236756316 1 7 -2.4421360453721874 1 8 -2.499271454810035 1 9 -2.4283933964178317 1 10 -2.8443920441529116 1 11 -1.6615568935686937 1 12 -1.9161431236538178 1 13 -1.9233785477727139 1 14 -1.8463221267339662 1 15 -2.246820609959684 1 16 -1.8067127413513688 1 17 -1.4515066162291532 1 18 -1.5333805986227844 1 19 -2.1241365991995593 1 20 -1.7790950199904074 1 21 -1.9338609986091342 1 22 -1.711745805120458 1 23 -1.3285799955014 1 24 -1.6474373191253875 1 25 -1.9231870529244226 1 26 -1.4521359383846821 1 27 -0.8663318823480566 1 28 -1.4079267476446624 1 29 -1.2684280089352917 1 30 -1.9593730877406834 1 31 -2.243648442764318 1 32 -2.3010747475713274 1 33 -2.118529171055787 1 34 -1.930615849100672 1 35 -2.3662983352677642 1 36 -1.668469254386153 1 37 -2.418576831195935 1 38 -2.668649530069647 1 39 -2.4203962397618355 1 40 -2.616934838441477 1 41 -2.4457498153334463 1 42 -2.535620867117295 1 43 -2.4801858569453827 1 44 -2.1686927550627537 1 45 -2.000127913402519 1 46 -1.3521285871149225 1 47 -1.7715341536621914 1 48 -1.6447636063007387 1 49 -1.499855381875573 1 50 -1.6366918864522049 1 51 -1.6178903205826494 1 52 -2.151016338917028 1 53 -2.2084073233224224 1 54 -1.714823106120459 1 55 -2.033265922454998 1 56 -2.3797962072375682 1 57 -1.7772164989103274 1 58 -2.1463229876279613 2 1 -0.6165637128996266 2 2 -1.1418118753077877 2 3 -1.1023014576980898 2 4 -1.240107592471432 2 5 -1.474523908491463 2 6 -0.8257934129534306 2 7 -1.0046651016250934 2 8 -0.9940247257139347 2 9 -1.0712173321287963 2 10 -0.7809829985262908 2 11 -0.689002599884688 2 12 -1.3095979951421626 2 13 -1.0575520219043497 2 14 -0.64832451764849 2 15 -0.8589777461753982 2 16 -0.8511937136566132 2 17 -1.0208099303023417 2 18 -1.1106536277786603 2 19 -1.004325215789848 2 20 -1.3740674580590806 2 21 -0.6181938670424216 2 22 -0.9879960541740544 2 23 -0.8205438661263642 2 24 -1.2774100892655227 2 25 -1.0641015777301632 2 26 -1.264117076725654 2 27 -1.358013492230505 2 28 -0.8269345181168681 2 29 -1.1673952666891587 2 30 -1.0013967312287626 2 31 -0.7763574817495069 2 32 -1.0116412614851207 2 33 -0.40165329936819916 2 34 -0.5993413588303653 2 35 -0.6986354542157613 2 36 -0.2084174512381125 2 37 -0.08831515888405406 2 38 0.06893099725983376 2 39 -0.32080800837443846 2 40 -0.47540598143144663 2 41 -0.35647240283427806 2 42 -0.9274817955891024 2 43 -0.8712664174820084 2 44 -0.9041148178906724 2 45 -0.49624267729971194 2 46 -1.3456199864640617 2 47 -1.0869522316325693 2 48 -1.4557817374025532 2 49 -0.8296355505678754 2 50 -1.0583728321917292 2 51 -1.4160240789674532 2 52 -1.4320816241664147 2 53 -1.2125914237408324 2 54 -1.7914818030720454 2 55 -1.6224935242349012 2 56 -1.5977845183221149 2 57 -2.267901894407437 2 58 -1.6960672926023932 3 1 -0.3734798126728691 3 2 -1.012106659888289 3 3 -0.7651132490813097 3 4 -0.790787864580274 3 5 -0.8574037270855612 3 6 -0.5688773131801881 3 7 -0.9484292739606985 3 8 -0.8060428663035069 3 9 -1.025865858205897 3 10 -1.001731297846021 3 11 -0.8811794706329863 3 12 -0.6922510563666506 3 13 -0.6828354686163678 3 14 -0.5391408441791015 3 15 -0.5713360228193878 3 16 -0.718880788486544 3 17 -0.6734176400529108 3 18 -0.9006763035156169 3 19 -1.2129419958351946 3 20 -0.697650224498993 3 21 -0.9478990824619231 3 22 -0.8730300677794958 3 23 -0.9373239114778329 3 24 -1.0259361663630244 3 25 -0.6447138226281197 3 26 -0.9493778477007098 3 27 -0.3842039684209837 3 28 -1.0024447221985007 3 29 -1.284515448095057 3 30 -0.7742992255598296 3 31 -0.4026613366247922 3 32 -0.34452108007922533 3 33 -0.63974853746344 3 34 -0.2587517896693683 3 35 -0.48639055625658045 3 36 -0.6846079274285906 3 37 -0.6156394219226051 3 38 -0.9853773927628424 3 39 -0.9872479176714926 3 40 -0.6200771832455061 3 41 -0.7851572100905138 3 42 -0.47612125137141703 3 43 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0.6082938344749387 117 51 0.4411187781754009 117 52 0.9885532964685062 117 53 0.8389958778464681 117 54 0.5497880381977964 117 55 0.8536969519555768 117 56 0.9299932594556637 117 57 0.34320921670367355 117 58 0.33964699311189506 118 1 0.0020464608286587804 118 2 -0.03919970182947452 118 3 0.018495942626870487 118 4 -0.13783912602973203 118 5 -0.06641867164606197 118 6 0.07359416359950188 118 7 -0.15120928360234842 118 8 0.0031006336161496506 118 9 -0.11127482494219265 118 10 -0.07616485079476121 118 11 -0.045489289048546766 118 12 0.10406904647763326 118 13 -0.046084705318918395 118 14 -0.19416253789595572 118 15 -0.1317249027808174 118 16 -0.1401888392659103 118 17 -0.40788654572541105 118 18 -0.13365075313798513 118 19 -0.24773228990558493 118 20 -0.3012015662955534 118 21 -0.400783543332885 118 22 -0.3526379739340868 118 23 -0.2079329424918157 118 24 -0.2865339487830809 118 25 -0.26348290506424377 118 26 0.38030237084340257 118 27 0.7943112171808195 118 28 0.2568675066300372 118 29 -0.12611917620046853 118 30 0.16211407991984572 118 31 0.7764484175130839 118 32 0.3146304545503753 118 33 0.6324674355399367 118 34 0.5655130343704862 118 35 0.6945029034895249 118 36 0.601668787981982 118 37 0.576695464787985 118 38 0.2408157616642832 118 39 0.7638689070111369 118 40 0.6628892316669166 118 41 0.4680807780181162 118 42 0.9070701354195201 118 43 1.0781774020405521 118 44 0.8337616975448974 118 45 1.1972331382233499 118 46 1.1653161465193165 118 47 1.2292894881524568 118 48 1.0455305985554517 118 49 1.2861624746789673 118 50 1.135884135687288 118 51 1.5514174908363216 118 52 1.0684183133413967 118 53 1.3818967652355454 118 54 1.2622302329234572 118 55 1.491179766603743 118 56 1.4169948842525635 118 57 1.2946215401632415 118 58 1.6998207213958594 119 1 -0.40682659011902444 119 2 -0.7582871874352719 119 3 -0.5594505887602637 119 4 -0.651221840542199 119 5 -1.0246054694425837 119 6 -0.5106858152371387 119 7 -0.6019422206413481 119 8 -0.4243214820976533 119 9 -0.6782817652801896 119 10 -0.3698687504555238 119 11 0.013472461286021087 119 12 -0.37862679243183095 119 13 -0.24942415942865637 119 14 -0.32350802978852045 119 15 -0.3950998366453149 119 16 -0.9705707134683905 119 17 -1.100488705633289 119 18 -0.4897865721289918 119 19 -0.7292765929151344 119 20 -0.853300785118499 119 21 -0.17111437648466676 119 22 -0.0919862668599194 119 23 -0.44583403489479423 119 24 -0.21282008989291512 119 25 0.13818526586103275 119 26 -0.8181041524518591 119 27 -0.3882694681386525 119 28 -0.4864489167617023 119 29 -0.869995806246223 119 30 -0.8639822133794617 119 31 -1.1707987892342935 119 32 -1.1858052617360784 119 33 -0.8706915075368435 119 34 -0.8565093116504912 119 35 -0.815518571098881 119 36 -0.4203504457798033 119 37 -0.18927820549927432 119 38 -0.4317434490667864 119 39 -0.5391717702164627 119 40 -0.42377162544926605 119 41 -0.2627243634340637 119 42 -0.15084900808675172 119 43 -0.248423077247363 119 44 0.12240817602362597 119 45 0.10680644748854462 119 46 0.6223202858240384 119 47 -0.032949471108072004 119 48 0.3788691189874349 119 49 -0.13909662089851338 119 50 -0.17627546096392166 119 51 -0.27031230282089425 119 52 0.1254815855707072 119 53 -0.007057826902887143 119 54 0.531923052941508 119 55 0.3140144122730355 119 56 -0.3986660039862109 119 57 -0.2938288659865833 119 58 -0.46967291403409916 120 1 -1.2998527652873904 120 2 -2.5220830109437316 120 3 -1.8726195992608652 120 4 -2.4494372470800556 120 5 -3.3790644085255224 120 6 -2.1722407186282027 120 7 -2.8024714917061644 120 8 -3.404168468475703 120 9 -3.353797889387465 120 10 -2.4901082795399887 120 11 -1.9327692052392749 120 12 -2.2459867137197236 120 13 -2.0046362919499927 120 14 -1.993358239291652 120 15 -2.017179939785315 120 16 -1.5299695158229696 120 17 -1.8404842950410867 120 18 -1.8939494926195835 120 19 -1.949212469689975 120 20 -2.4183824658934014 120 21 -2.1165248160171473 120 22 -1.9112537692772662 120 23 -2.1090853199038655 120 24 -2.210818359583662 120 25 -2.7353155568840535 120 26 -1.97649530412534 120 27 -1.6093419205975614 120 28 -2.0016739417833307 120 29 -2.2815549505913353 120 30 -2.0552662046261325 120 31 -1.5700082754003013 120 32 -1.2639745266939215 120 33 -1.4460023694683422 120 34 -1.443949302150756 120 35 -1.9353000771464792 120 36 -1.9304693621312268 120 37 -2.0396912744232587 120 38 -1.8592146529888218 120 39 -1.9831487672817225 120 40 -1.7509697119308 120 41 -1.7455145719193652 120 42 -1.3535496476226214 120 43 -1.6996266600053396 120 44 -2.0221235378307227 120 45 -1.4312518741114815 120 46 -1.3451431147425552 120 47 -1.2826229176466057 120 48 -1.0358961866157124 120 49 -1.2949601639611608 120 50 -1.359840915848654 120 51 -1.3964382761198486 120 52 -1.6818942817043947 120 53 -1.7791150288910487 120 54 -1.4893665363645034 120 55 -1.6688182057527143 120 56 -1.8216587604367014 120 57 -1.7029621846179417 120 58 -1.50841827018942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 901.0 3 65.0 4 458.0 5 319.0 6 811.0 7 2944.0 8 1268.0 9 1764.0 10 2067.0 11 1786.0 12 2021.0 13 2219.0 14 2227.0 15 2435.0 16 2905.0 17 3010.0 18 3369.0 19 3927.0 20 3628.0 21 2717.0 22 3449.0 23 3288.0 24 3475.0 25 3307.0 26 3827.0 27 4631.0 28 5681.0 29 6925.0 30 8647.0 31 10485.0 32 12824.0 33 15557.0 34 18745.0 35 23104.0 36 28842.0 37 42209.0 38 46199.0 39 30079.0 40 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 41.23705065699697 39.43935831480758 5.640692169211156 13.682898858984297 11 29.399252990281187 35.16038926509876 16.36982747022532 19.070530274394738 12 48.06360473832237 12.562704610830998 23.30241080409254 16.071279846754095 13 22.833447584390957 15.932576927265854 26.5409477926055 34.69302769573769 14 20.567432682636188 34.95537802151337 29.093658105311714 15.383531190538733 15 42.02666427486882 14.926420183356932 27.13323808852642 15.913677453247827 16 24.26756529928438 18.23350780644376 37.24125338749047 20.257673506781387 17 39.13696673051913 14.865877800485618 23.244110731697944 22.753044737297312 18 39.37162776987453 18.69192715342125 20.66592333423046 21.270521742473758 19 27.312740051189564 20.74971249915912 35.99638661895808 15.941160830693239 20 28.497203518505472 34.29806072176771 20.778530197515522 16.4262055622113 21 27.90005605830063 21.10066469127893 34.91246896772643 16.086810282694003 22 29.666918661268006 19.863474450310083 21.195735738814005 29.273871149607917 23 31.1224081210339 34.658543070117275 19.744567985469782 14.474480823379043 24 29.262893516961235 33.9346410253946 20.28043939990966 16.52202605773451 25 22.582773819896467 19.168033417046797 22.29927989339347 35.949912869663265 26 34.20225597390989 17.503179571294478 25.78768472748591 22.506879727309716 27 20.255462141679974 18.405995907208645 24.457480266898827 36.881061684212554 28 22.335814129323907 34.35800993813495 25.37364119552367 17.93253473701747 29 36.267373237044964 17.65229816928213 27.141337571046847 18.93899102262606 30 21.712259826517332 30.125143446958287 30.667196645745314 17.49540008077907 31 39.524386180737096 17.560164539443335 28.98117535496438 13.934273924855194 32 30.50026275650803 16.236429587664546 40.07549443084377 13.187813224983657 33 32.56583058538508 16.13710579360425 34.234788544180276 17.062275076830392 34 24.205001345826016 20.555569797100706 38.05419192760738 17.1852369294659 35 20.103381862347657 36.456687614365784 33.75933753569174 9.680592987594817 36 25.864257280402047 28.881866141883705 31.19114621060121 14.062730367113033 37 22.25983376127144 26.260013270889633 39.73124845733922 11.74890451049971 38 25.59521710557951 26.580647229479553 35.18056067576015 12.643574989180786 39 23.35936798635425 23.102868941813192 34.762033011427036 18.775730060405525 40 21.155424204736324 23.086046183631865 39.65964894285385 16.09888066877797 41 23.367521312715812 23.954562796766943 36.74603963411702 15.931876256400226 42 18.258413457685045 26.152331915193756 36.65180131511874 18.93745331200246 43 22.247332923013797 19.55140534441196 37.365684464276555 20.835577268297687 44 19.233568101455734 23.343666550190903 38.21003465421124 19.212730694142124 45 21.253734405251887 21.560821120385683 41.71539023733508 15.47005423702735 46 18.22282493700348 24.401613223818774 38.49712428107026 18.878437558107475 47 23.021149005082815 21.51522439751792 39.45067102794747 16.01295556945179 48 24.878222416054438 20.18734026212244 39.18656742742249 15.747869894400637 49 23.36826846601842 20.907201683043848 38.11767196038689 17.606857890550838 50 15.65802513879579 22.78624326066667 42.096417769709646 19.4593138308279 51 14.026478851556787 24.42943463033522 43.87901063515889 17.665075882949107 52 17.47596477613089 21.53774123411815 44.30530346273979 16.680990527011165 53 13.12490579712469 21.818187648920713 45.67179878586524 19.38510776808936 54 13.625355645582937 24.243240729157645 43.22854017712513 18.902863448134283 55 17.869623116787267 21.878158743401734 38.48540760825966 21.766810531551332 56 16.85801496336191 23.86210084060585 38.687127079448324 20.59275711658391 57 21.189840430311822 25.977893885515492 35.79662888172844 17.035636802444255 58 19.6947427199733 28.789561176614058 36.2252331469869 15.290462956425744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 539.0 10 1078.0 11 948.5 12 819.0 13 767.5 14 716.0 15 832.5 16 2190.5 17 3432.0 18 3727.5 19 4023.0 20 4376.0 21 4729.0 22 5155.5 23 6427.0 24 7272.0 25 8262.5 26 9253.0 27 9874.0 28 10704.0 29 10913.0 30 10808.5 31 10704.0 32 9886.5 33 9069.0 34 13102.0 35 11764.0 36 6393.0 37 5685.0 38 4977.0 39 4661.5 40 4346.0 41 4482.5 42 4903.5 43 5188.0 44 6068.0 45 6948.0 46 7049.5 47 6607.5 48 6064.0 49 6448.0 50 6832.0 51 7207.0 52 7582.0 53 9719.5 54 12608.0 55 13359.0 56 15368.0 57 17377.0 58 17826.0 59 21321.0 60 24367.0 61 24262.0 62 24157.0 63 21713.0 64 19269.0 65 14990.0 66 8579.0 67 6447.0 68 5011.0 69 3575.0 70 2916.0 71 2257.0 72 1989.5 73 1436.0 74 1150.0 75 903.5 76 657.0 77 489.0 78 254.0 79 187.0 80 145.5 81 104.0 82 82.5 83 61.0 84 50.0 85 23.5 86 8.0 87 7.0 88 6.0 89 4.5 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.022423104767152074 19 0.0016016503405108624 20 0.0 21 9.609902043065175E-4 22 6.406601362043449E-4 23 3.2033006810217247E-4 24 0.00800825170255431 25 6.406601362043449E-4 26 0.009289571974963003 27 0.2879767312238531 28 0.015055513200802107 29 0.01921980408613035 30 0.06919129471006925 31 0.010891222315473865 32 0.03267366694642159 33 0.040681918648975905 34 0.03267366694642159 35 0.041963238921384594 36 0.056378091985982356 37 0.06951162477817142 38 0.07463690586780619 39 0.09161439947722133 40 0.06598799402904754 41 0.08809076872809743 42 0.08552812818328005 43 0.07239459539109099 44 0.07655888627641923 45 0.06855063457386491 46 0.08200449743415615 47 0.10987321335904517 48 0.10859189308663646 49 0.11595948465298643 50 0.12460839649174509 51 0.12236608601502989 52 0.11019354342714732 53 0.11243585390386254 54 0.1348589586710146 55 0.17393922697947964 56 0.1976436520190404 57 0.18899474018028176 58 0.18322879895444266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 312178.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.14833714307409 #Duplication Level Percentage of deduplicated Percentage of total 1 71.43871191861818 24.39513219663919 2 12.229736003031624 8.352502964046305 3 5.331789358264087 5.4621522174557 4 2.770489426948539 3.7843042801104336 5 1.6895436465851597 2.884755303076443 6 1.1755973444002021 2.4086816676648413 7 0.8540460958426288 2.0414977811592174 8 0.687811354132037 1.87900912093881 9 0.5484567478593653 1.6855997340866025 >10 2.8617291154048625 17.37877003433938 >50 0.227942943142867 5.326344176136772 >100 0.15926144499064943 10.376683791263776 >500 0.012939992405490265 3.183909757362676 >1k 0.010949224343107147 7.613239110217703 >5k 9.953840311915589E-4 3.227417865502147 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 9495 3.041533996630128 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 4310 1.3806225935203633 No Hit GAGATCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 2922 0.936004458994548 No Hit GAGATCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 2678 0.8578439223776179 No Hit GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 2081 0.6666068717206209 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGTGG 2076 0.6650052213801101 No Hit GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1946 0.6233623125268276 No Hit GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1510 0.48369840283428045 No Hit GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1432 0.458712657522311 No Hit GAGATCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 1346 0.4311642716655241 No Hit GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1086 0.3478784539589593 No Hit GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 1011 0.3238536988512964 No Hit GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 986 0.3158454471487421 No Hit GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 921 0.2950239927221009 No Hit GAGATCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 862 0.2761245187040727 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 844 0.27035857747823355 No Hit GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.26235032577567924 No Hit GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 0.2613893355713727 No Hit GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.2306376490335642 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 678 0.2171837861732729 No Hit GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 580 0.18579143949926005 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 544 0.17425955704758184 Illumina Single End Adapter 2 (96% over 25bp) GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGGGG 539 0.17265790670707096 No Hit GAGATCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 535 0.17137658643466228 No Hit GAGATCGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 523 0.1675326256174362 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCCAA 496 0.15888371377867755 No Hit GAGATCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 463 0.14831282153130587 No Hit GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG 425 0.13614027894342332 No Hit GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 419 0.13421829853481027 No Hit GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 416 0.13325730833050375 No Hit GAGATCGGGAGCCATGTTGTTTGCCTTGCCTCAGAATTTACCTTTATGATTGGAAGAG 405 0.12973367758137985 No Hit GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 399 0.1278116971727668 No Hit GAGATCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 398 0.12749136710466466 No Hit GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 395 0.12653037690035812 No Hit GAGATCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA 392 0.1255693866960516 No Hit GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 392 0.1255693866960516 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA 391 0.12524905662794944 No Hit GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAA 385 0.1233270762193364 No Hit GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 385 0.1233270762193364 No Hit GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA 371 0.118842455265906 No Hit GAGATCGGGGCCATTGCTTTCTGGATATGGATTAACAAACATCCTTGCAGAGATTAGA 335 0.10731057281422778 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGAGATCGGGAAGCAGTGG 327 0.10474793226941041 No Hit GAGATCGGGAGGAGCAGGAAGGCGTCATCTGTTAGAAAGAGGGAAAGGAACTCTTGAT 326 0.10442760220130823 No Hit GAGATCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 323 0.1034666119970017 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.020501124358539038 0.0 0.0 0.0 0.0 11 0.03715828789985201 0.0 0.0 0.0 0.0 12 0.0384396081722607 0.0 0.0 0.0 0.0 13 0.040681918648975905 0.0 0.0 0.0 0.0 14 0.06951162477817142 0.0 0.0 0.0 0.0 15 0.07239459539109099 0.0 0.0 0.0 0.0 16 0.07431657579970402 0.0 0.0 0.0 0.0 17 0.0755978960721127 0.0 0.0 0.0 0.0 18 0.07751987648072574 0.0 0.0 0.0 0.0 19 0.08168416736605398 0.0 0.0 0.0 0.0 20 0.10346661199700172 0.0 0.0 0.0 0.0 21 0.11499849444867992 0.0 0.0 0.0 0.0 22 0.1313353279218907 0.0 0.0 0.0 0.0 23 0.15215678234853192 0.0 0.0 0.0 0.0 24 0.174579887115684 0.0 0.0 0.0 0.0 25 0.1813068185458296 0.0 0.0 0.0 0.0 26 0.18451011922685134 0.0 0.0 0.0 0.0 27 0.18899474018028176 0.0 0.0 0.0 0.0 28 0.30815752551428993 0.0 0.0 0.0 0.0 29 0.31104049612720946 0.0 0.0 0.0 0.0 30 0.36421528743217013 0.0 0.0 0.0 0.0 31 0.3667779279769875 0.0 0.0 0.0 0.0 32 0.369660898589907 0.0 0.0 0.0 0.0 33 0.37446584961143964 0.0 0.0 0.0 0.0 34 0.38151311110968744 0.0 0.0 0.0 0.0 35 0.5602572891106997 0.0 0.0 0.0 0.0 36 0.568265540813254 0.0 0.0 0.0 0.0 37 0.5727501617666844 0.0 0.0 0.0 0.0 38 0.5797974232649322 0.0 0.0 0.0 0.0 39 0.58684468476318 0.0 0.0 0.0 0.0 40 0.5954935966019387 0.0 0.0 0.0 0.0 41 0.605423828713106 0.0 0.0 0.0 0.0 42 0.6198386817777037 0.0 0.0 0.0 0.0 43 0.6288079236845646 0.0 0.0 0.0 0.0 44 0.6377771655914254 0.0 0.0 0.0 0.0 45 0.6461057473620819 0.0 0.0 0.0 0.0 46 0.655715649405147 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTATTA 20 3.3981245E-4 52.140697 27 CCTCGAC 15 0.0071385335 52.140697 27 ACGCGAT 45 9.094947E-11 52.123936 24 CTCGTGA 15 0.007147617 52.123936 26 ACACCTC 15 0.007147617 52.123936 24 CGCGATC 50 3.6379788E-12 52.123936 25 ACCTCGA 15 0.007147617 52.123936 26 ACGTGCC 15 0.007147617 52.123936 26 GCTACTA 20 3.403524E-4 52.123936 24 CCACGTG 15 0.007147617 52.123936 24 CCTAATC 15 0.0071521625 52.11556 22 TATGACA 15 0.0071521625 52.11556 21 AAATTGA 25 1.630822E-5 52.11556 23 TGCTACT 20 3.4062265E-4 52.11556 23 ATAACTC 15 0.0071521625 52.11556 22 GCCACGT 15 0.0071521625 52.11556 23 GCGTCAT 40 1.8517312E-9 52.11556 22 GAGGGAC 15 0.0071521625 52.11556 21 CTTAATA 15 0.0071794786 52.06535 52 GTCGAAC 35 3.8329745E-8 52.06535 52 >>END_MODULE