FastQCFastQC Report
Tue 31 May 2016
SRR212685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317313
Sequences flagged as poor quality0
Sequence length58
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT82472.5990110710875385No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA39931.2583789507520966No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGTGG34081.0740183982377023No Hit
GAGATCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT28660.9032091341987248No Hit
GAGATCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC24770.7806172454327431No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA19440.6126442975862949No Hit
GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC16740.5275548118104206No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC15300.4821737527299543No Hit
GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG12950.4081143854805822No Hit
GAGATCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT11600.3655696425926451No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10350.32617636214085144No Hit
GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC9290.2927708603177304No Hit
GAGATCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT9050.2852073504709861No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.2798498643295421No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA7780.24518377753196371No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7630.24045658387774846No Hit
GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.23982629139051975No Hit
GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.23068705032570364No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGGGG6830.21524488438860054No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA6250.19696640225896825No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.19223920860475305No Hit
GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5490.17301528774427774No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC5110.16103973048693246Illumina Single End Adapter 2 (96% over 25bp)
GAGATCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4740.14937931947320154No Hit
GAGATCGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4530.14276124835730022No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCCAA4220.13299171480525537No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG4040.1273190824201971No Hit
GAGATCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3920.1235373274968249No Hit
GAGATCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA3890.12259188876598186No Hit
GAGATCGGGAGCCATGTTGTTTGCCTTGCCTCAGAATTTACCTTTATGATTGGAAGAG3860.12164645003513881No Hit
GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3790.11944042632983835No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.11471323267562311No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAA3590.11313750145755137No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3510.11061633150863659No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGAGATCGGGAAGCAGTGG3470.10935574653417918No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.10841030780333613No Hit
GAGATCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT3390.10683457658526439No Hit
GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3360.10588913785442135No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAA3330.1049436991235783No Hit
GAGATCGGGAGGAGCAGGAAGGCGTCATCTGTTAGAAAGAGGGAAAGGAACTCTTGAT3240.10210738293104915No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3240.10210738293104915No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA3210.1011619442002061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG150.007029744352.3437279
GGTACCT150.007029744352.3437278
GGGCTAT307.621984E-752.3437277
GGATCTA150.007029744352.3437278
GGATAGC150.007029744352.3437278
CTACGAA150.007029744352.34372710
CGGGCGA150.007029744352.3437276
GGATTGA203.3336124E-452.3437278
GGCTATC150.007029744352.3437278
GAACTGT251.5892378E-552.3437239
TGCATAC150.00720380152.02102351
CCCGTCA203.4369781E-452.02102352
CCCCGTC203.4369781E-452.02102351
CAGCTAA150.00720380152.02102351
CAATGCG150.00720380152.02102352
TCGCCCG150.00720830652.01280243
CTCACAC150.00720830652.01280244
GCGCGAA150.00720830652.01280239
TAGCGGT150.00720830652.01280227
ACACAAT150.00720830652.01280244
Produced by FastQC (version 0.11.5)