FastQCFastQC Report
Tue 31 May 2016
SRR212684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319672
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT103983.2527090267524215No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC47031.4711954753622463No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGT34501.079231211992292No Hit
GAGATCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT28760.8996721639680673No Hit
GAGATCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC22060.6900823343927526No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG20160.6306464125728872No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC19900.6225130759028004No Hit
GAGATCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC17090.5346104757376311No Hit
GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA14920.4667283966065217No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13900.4348206912084887No Hit
GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA13410.41949247979178655No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGG11850.3706924597712655No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.34848219424910537No Hit
GAGATCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA10970.34316424334943313No Hit
GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.3175129507745439No Hit
GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT9700.30343602192247054No Hit
GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.28091293575915316No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8470.26495908306013666No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8330.260579594083936No Hit
GAGATCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7470.233677018944418No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.21960009009234466No Hit
GAGATCGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT5950.18612828148852575No Hit
GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5570.17424109712455269No Hit
GAGATCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4780.14952826647313497No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG4770.1492154458319778Illumina Single End Adapter 2 (95% over 23bp)
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC4320.1351385169799044No Hit
GAGATCGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4140.12950774543907503No Hit
GAGATCGGGAGCCATGTTGTTTGCCTTGCCTCAGAATTTACCTTTATGATTGGAAG4080.12763082159213193No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGAGATCGGGAAGCAGT4050.12669235966866038No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCC4040.1263795390275032No Hit
GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4010.12544107710403163No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC4000.12512825646287445No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG3890.1216872294101454No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3890.1216872294101454No Hit
GAGATCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3810.11918466428088792No Hit
GAGATCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG3780.11824620235741634No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3710.11605645786931604No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.11480517530468731No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.11386671338121575No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3600.11261543081658701No Hit
GAGATCGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3590.11230261017542982No Hit
GAGATCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3510.10980004504617233No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.10948722440501514No Hit
GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.10917440376385795No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3460.1082359418403864No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA3320.1038564528641858No Hit
GAGATCGGGGCCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC3230.10104106709377111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACC150.00825659350.25670250
CGGAGTT150.00825659350.25670250
GTTTGAT301.0102849E-650.2487848
CCTGTCA150.00826174150.2487849
CAGTAAG150.00826174150.2487849
CCAGTAA150.00826174150.2487848
TTATTGC150.00826174150.2487848
AATTCTG150.00827204350.2329447
CAATGAG204.0845317E-450.23293747
ATGAAAG252.029407E-550.22502546
CTAAACG204.0908897E-450.21711345
CGACCCA150.00828235650.2171145
GGTCAAT252.0331945E-550.209242
ATACTGC150.00828751650.209242
TCAATTT252.0331945E-550.209244
ACGTGTG204.0940716E-450.209242
GTCAATT252.0331945E-550.209243
CTGTTAT150.00828751650.209242
CATAATT150.00828751650.209244
TTCGGGT355.102629E-850.209244
Produced by FastQC (version 0.11.5)