FastQCFastQC Report
Tue 31 May 2016
SRR212683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291675
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT91173.12573926459244No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC45111.5465843833033341No Hit
GAGATCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT28630.9815719550869975No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGT23220.7960915402417075No Hit
GAGATCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC22930.7861489671723666No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC21230.7278649181451958No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG18620.6383817605211279No Hit
GAGATCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC17740.6082111939658866No Hit
GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA13730.4707294077312077No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13220.4532441930230565No Hit
GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA12750.4371303677037799No Hit
GAGATCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC10120.3469615153852747No Hit
GAGATCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA9800.33599040027427785No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.32639067455215565No Hit
GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT9380.32159081169109455No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGG9360.32090511699665725No Hit
GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.31781949087168937No Hit
GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.290734550441416No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8050.27599211451101396No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7230.2478786320390846No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.22216508099768575No Hit
GAGATCGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT6290.2156509814005314No Hit
GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5740.19679437730350563No Hit
GAGATCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4840.16593811605382702No Hit
GAGATCGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT4240.145367275220708No Hit
GAGATCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4200.14399588583183337No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG4170.14296734379017742Illumina Single End Adapter 2 (95% over 23bp)
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCC4170.14296734379017742No Hit
GAGATCGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4110.14091025970686552No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC3870.1326819233736179No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG3820.13096768663752464No Hit
GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3760.12891060255421274No Hit
GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.12685351847090084No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.12582497642924487No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC3460.1186251821376532No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3390.11622525070712265No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3360.1151967086654667No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3330.11416816662381075No Hit
GAGATCGGGGCCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC3220.11039684580440559No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3170.10868260906831233No Hit
GAGATCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG3160.10833976172109369No Hit
GAGATCGGGAGCCATGTTGTTTGCCTTGCCTCAGAATTTACCTTTATGATTGGAAG3120.10696837233221909No Hit
GAGATCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3110.10662552498500043No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.10559698294334448No Hit
GAGATCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3010.10319705151281393No Hit
GAGATCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2980.10216850947115798No Hit
GAGATCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC2970.10182566212393931No Hit
GAGATCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2940.10079712008228336No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGAGATCGGGAAGCAGT2930.10045427273506471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG451.2914825E-1050.27464750
GAGACTA150.0082442250.27464350
TGTCCTA150.008255550.2572648
GGAACTC252.0233394E-550.24857347
GTTGTCC150.00826679250.23988746
ACATCCC150.00826679250.23988746
CACATCC150.00827244150.2312145
CCACATC150.00827244150.2312144
TTAAGCG204.0846269E-450.23120544
TAAGCGT204.0846269E-450.23120545
GCCACAT150.00827809450.22252743
TGGTCGG204.0881115E-450.22252742
ACACCAT150.00829506950.1965141
TAAGTTA301.0172498E-650.1965141
ACTCGGT150.00829506950.1965141
CCGGGAT252.03787E-550.18784339
TCTAGTT150.00830073450.18784339
TGACGCT150.00830073450.18784338
TGTGATT150.00830073450.18784340
TTGACGC150.00830073450.18784337
Produced by FastQC (version 0.11.5)