##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212683_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 291675 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.975650981400534 39.0 38.0 40.0 35.0 40.0 2 37.000514271020826 39.0 36.0 40.0 32.0 40.0 3 37.461592525927834 39.0 38.0 40.0 33.0 40.0 4 37.05267506642667 39.0 36.0 40.0 33.0 40.0 5 37.309028884889 39.0 37.0 40.0 33.0 40.0 6 37.66793520185138 39.0 38.0 40.0 34.0 40.0 7 37.88892088797463 39.0 38.0 40.0 35.0 40.0 8 37.7033959029742 39.0 38.0 40.0 35.0 40.0 9 37.524604439873144 39.0 38.0 40.0 34.0 40.0 10 36.51806291248821 39.0 35.0 40.0 31.0 40.0 11 36.35102425644982 39.0 36.0 40.0 30.0 40.0 12 36.306481529099166 39.0 35.0 40.0 30.0 40.0 13 36.28160795405846 39.0 35.0 40.0 30.0 40.0 14 36.030348847175794 39.0 35.0 40.0 30.0 40.0 15 36.04497128653467 39.0 35.0 40.0 30.0 40.0 16 35.64360675409274 38.0 35.0 40.0 29.0 40.0 17 35.71804577012085 38.0 35.0 40.0 29.0 40.0 18 35.53067626639239 38.0 35.0 40.0 29.0 40.0 19 35.05112539641725 38.0 35.0 40.0 28.0 40.0 20 35.211835090425986 38.0 35.0 40.0 28.0 40.0 21 34.88380217708065 38.0 35.0 40.0 27.0 40.0 22 34.74797634353304 38.0 35.0 39.0 27.0 40.0 23 34.365074140738834 38.0 34.0 39.0 26.0 40.0 24 34.299316019542296 38.0 34.0 39.0 26.0 40.0 25 34.203178194908716 38.0 34.0 39.0 25.0 40.0 26 33.59066769520871 38.0 33.0 39.0 22.0 40.0 27 33.477322362218224 38.0 33.0 39.0 21.0 40.0 28 32.8434764721008 37.0 33.0 39.0 17.0 40.0 29 32.9391925944973 38.0 33.0 39.0 17.0 40.0 30 32.23706865518128 37.0 32.0 39.0 9.0 40.0 31 31.356297248650037 36.0 31.0 39.0 2.0 40.0 32 30.940550269992286 36.0 30.0 39.0 2.0 40.0 33 30.790520270849406 36.0 30.0 39.0 2.0 40.0 34 30.75742864489586 36.0 30.0 39.0 2.0 40.0 35 30.28026742093083 35.0 29.0 39.0 2.0 40.0 36 30.24001714236736 36.0 30.0 39.0 2.0 40.0 37 29.911325962115367 36.0 29.0 39.0 2.0 40.0 38 29.830708836890373 36.0 29.0 39.0 2.0 40.0 39 29.75964001028542 36.0 29.0 39.0 2.0 40.0 40 29.557685780406274 36.0 28.0 39.0 2.0 40.0 41 29.253199622867918 36.0 27.0 39.0 2.0 40.0 42 29.017413216765235 35.0 26.0 39.0 2.0 40.0 43 28.86375246421531 35.0 26.0 39.0 2.0 40.0 44 28.6757966915231 35.0 26.0 39.0 2.0 40.0 45 28.23870060855404 35.0 23.0 39.0 2.0 40.0 46 27.489418016628097 35.0 19.0 39.0 2.0 40.0 47 27.39120253707037 35.0 19.0 39.0 2.0 40.0 48 27.27404131310534 35.0 18.0 39.0 2.0 40.0 49 27.002125653552756 34.0 15.0 39.0 2.0 40.0 50 26.606798662895347 34.0 2.0 39.0 2.0 40.0 51 25.98411588240336 34.0 2.0 39.0 2.0 40.0 52 25.803473043627324 34.0 2.0 39.0 2.0 40.0 53 25.46950544270164 33.0 2.0 38.0 2.0 40.0 54 25.146872375075 33.0 2.0 38.0 2.0 40.0 55 24.735702408502615 33.0 2.0 38.0 2.0 40.0 56 14.072779634867576 18.0 2.0 22.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5285921438063426 1 2 -0.6824023631477942 1 3 -0.561821990098494 1 4 -1.0849175364689643 1 5 -0.7754472858077577 1 6 -0.858307181699665 1 7 -0.5093949045527282 1 8 -0.6150798142836393 1 9 -0.8033731553938139 1 10 -0.5190113757777937 1 11 -0.18739036432469902 1 12 -0.2141464218824538 1 13 -0.27876004018943945 1 14 -0.9957316629058397 1 15 -1.0837444207746287 1 16 -1.0636668278753518 1 17 -1.0587585642737878 1 18 -1.173195843050003 1 19 -1.2411242555167021 1 20 -1.0662837823345157 1 21 -1.1805784693488164 1 22 -0.8684488578578495 1 23 -0.9835026556533606 1 24 -1.1292660736988722 1 25 -1.1807643019892566 1 26 -1.312358964305652 1 27 -0.692456140937523 1 28 -0.8279264052825646 1 29 -1.0172888612156967 1 30 -0.9969593789876221 1 31 -0.7468543050343399 1 32 -0.886872433802278 1 33 -1.3226239353081617 1 34 -0.930409639854247 1 35 -1.1930219450721538 1 36 -1.037525029540376 1 37 -1.167092757395693 1 38 -1.4973370667126318 1 39 -1.439071475062697 1 40 -1.3497939739425036 1 41 -1.089236976904015 1 42 -1.0533760849587708 1 43 -1.241803027699131 1 44 -1.0227554143756308 1 45 -1.5147100050056501 1 46 -0.8990187754675922 1 47 -1.0433552551310825 1 48 -1.598837284950374 1 49 -1.1529132929411219 1 50 -1.2830218289158175 1 51 -1.8132218231147164 1 52 -1.3090191336836874 1 53 -1.6126506252841004 1 54 -1.8419305534564963 1 55 -1.6546875828443817 1 56 -1.1363229244231654 2 1 -0.6121536245824402 2 2 -0.9414191468640212 2 3 -0.8510335918919338 2 4 -0.598269121597717 2 5 -1.1201549264325479 2 6 -0.9070447658496974 2 7 -0.7542731597868908 2 8 -0.8277264568790983 2 9 -0.7945514542735808 2 10 -0.9520286091759331 2 11 -1.4370431834335378 2 12 -1.6504172362035021 2 13 -1.3311744353011363 2 14 -1.500856714156349 2 15 -1.4259920367991796 2 16 -0.8065942571496478 2 17 -1.233724507481604 2 18 -1.03854256103331 2 19 -0.8014279230695109 2 20 -1.1395687087321633 2 21 -1.3872998914017458 2 22 -1.2460494080568978 2 23 -0.8717566994841235 2 24 -1.498825253161634 2 25 -0.8988930961804229 2 26 -1.396502386385034 2 27 -1.2007950952948114 2 28 -1.69385506456916 2 29 -1.5955136757219073 2 30 -1.4589678700402722 2 31 -1.5006534559290756 2 32 -1.531610494086106 2 33 -1.2163402918265653 2 34 -1.5252991371363152 2 35 -1.5511935913047452 2 36 -1.7558389868734956 2 37 -1.4926227868895374 2 38 -1.639357196591348 2 39 -1.474080534830712 2 40 -1.61086077815893 2 41 -1.4057601421356694 2 42 -0.6752464972112797 2 43 -1.1434152696280009 2 44 -0.6718580344340914 2 45 -1.0845231224916638 2 46 -1.2688424737045736 2 47 -1.0948041567168723 2 48 -1.6524684664234783 2 49 -1.2307743941327765 2 50 -1.1558808800869755 2 51 -1.126054951446001 2 52 -0.9255594926356636 2 53 -1.2402214171210524 2 54 -1.1240662780395496 2 55 -0.5473855420820399 2 56 -0.8446248461520618 3 1 -0.4295646295748483 3 2 -0.6582088147606981 3 3 -0.551543685228161 3 4 -0.6914561196441724 3 5 -0.793328900819354 3 6 -0.3065862244995827 3 7 -0.26561459673067844 3 8 -0.26461597283307725 3 9 -0.30073769260654615 3 10 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1.7883539993950457 4 35 1.8104300249314171 4 36 1.5356739282556546 4 37 1.6771189105274367 4 38 1.962118818168797 4 39 1.7694619005936403 4 40 2.023825568704538 4 41 1.6828007434731873 4 42 1.3764140193938879 4 43 1.8307544720694153 4 44 1.8299869840160952 4 45 2.0040377631171893 4 46 2.2403826185463487 4 47 2.097077762102316 4 48 2.1704097623588083 4 49 2.4666425800369645 4 50 2.2315729944517706 4 51 3.0732070411739265 4 52 2.5762855258145194 4 53 2.254243527528395 4 54 2.311358076203991 4 55 2.098370915482537 4 56 0.9081426815232163 5 1 0.08563510703621802 5 2 0.2261195434482559 5 3 0.32663893110195374 5 4 0.42800173812580766 5 5 0.2280165776705516 5 6 0.21245460078575462 5 7 0.10115449896730411 5 8 0.14802669617482422 5 9 0.12726933109845362 5 10 0.43302949513645217 5 11 0.3181063456736908 5 12 0.2697512938400095 5 13 0.02476266884602296 5 14 0.13030482133959964 5 15 0.5528040198181046 5 16 0.91643267461221 5 17 0.6536605230851151 5 18 0.9613197510226428 5 19 0.6421475574614135 5 20 0.5972132486396475 5 21 1.2661395534404605 5 22 0.6018742577469283 5 23 0.33371501941217474 5 24 0.8186556082141436 5 25 0.8006993475052084 5 26 0.3551636377378955 5 27 -0.14225018148746216 5 28 -0.15504083314326067 5 29 0.019703595853791 5 30 0.32148870318217604 5 31 0.46346506585315694 5 32 0.629002492640776 5 33 0.31955495518560895 5 34 0.27184521490421787 5 35 1.1956395859822528 5 36 0.37931549899971984 5 37 1.1531474394694428 5 38 0.7203112508393943 5 39 0.42036788726544927 5 40 0.7979484965009789 5 41 1.0732837527808918 5 42 0.7083796524830532 5 43 1.057774354208533 5 44 0.6894764371195805 5 45 0.5358436781912665 5 46 0.643684375444515 5 47 0.4233568831024819 5 48 0.581724957600315 5 49 0.1394822561949205 5 50 0.44552629070114946 5 51 1.1291496528388478 5 52 0.8486735855160106 5 53 0.8216830825209591 5 54 0.5139245794809675 5 55 0.7123214579730366 5 56 0.5588972114565749 6 1 -0.09028274893938004 6 2 -0.4775518804541292 6 3 -0.5357643808056594 6 4 0.04756813969460438 6 5 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0.3743881819498469 6 46 0.6123356446459525 6 47 0.1283084028758772 6 48 0.3885394254072665 6 49 0.17350012306495088 6 50 0.36537050031003915 6 51 0.20462083706371104 6 52 0.3646066936977306 6 53 0.5349960795794644 6 54 0.5511835401520635 6 55 0.43217111479839687 6 56 0.6220032142891281 7 1 -0.2571072878595331 7 2 -0.16011721170726645 7 3 -0.7790919564450434 7 4 -0.27719365893469217 7 5 -0.6318729062077679 7 6 -0.410565793426386 7 7 -0.1444875199457627 7 8 -0.13495723911058377 7 9 -0.1565975938189439 7 10 -0.3887205333470831 7 11 -0.619714094112112 7 12 -0.30760999133715927 7 13 -0.5099017950938176 7 14 -0.10217780370312113 7 15 -0.17808352717973008 7 16 -0.25196199326975943 7 17 -0.07930632207838073 7 18 0.15950819810023376 7 19 -0.12724973090590908 7 20 -0.3976881983240048 7 21 0.5759264965873214 7 22 -0.025703502307763415 7 23 0.7244236898006875 7 24 0.34565800083120735 7 25 -0.1833498720458948 7 26 0.7994498403128034 7 27 0.8147396783735346 7 28 0.417620668508782 7 29 0.25423703552566224 7 30 0.40548665236755 7 31 0.13732857274617416 7 32 0.4230950071673796 7 33 0.4014451226688003 7 34 -0.13253555301192677 7 35 0.11847768556069482 7 36 0.7657801224473673 7 37 0.26004615201359016 7 38 0.6437475046846721 7 39 0.5836924856475818 7 40 0.6207524158356037 7 41 0.5261150163105732 7 42 0.1317589392552989 7 43 0.8594719729848812 7 44 0.6087256110970216 7 45 0.47186965517656887 7 46 0.546697933607895 7 47 0.5601633089883187 7 48 0.6463541019548771 7 49 1.004669677865195 7 50 0.9950021936292579 7 51 0.7601454372754439 7 52 1.4350641517687173 7 53 1.1673023146048145 7 54 1.4253407809165353 7 55 1.062555022972468 7 56 0.7600764298683327 8 1 -0.46125496730689974 8 2 -0.6458514003120257 8 3 -0.38217441660816576 8 4 -0.3747903849092111 8 5 -0.3755672143957369 8 6 -0.06521125245753012 8 7 -0.26119724043454795 8 8 -0.433537914233213 8 9 -0.45819687399773557 8 10 -0.23268187198232937 8 11 -0.9262186075071739 8 12 -0.7862330430307694 8 13 -0.6149144209899617 8 14 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5120.0 29 5964.0 30 7315.0 31 8737.0 32 10616.0 33 13647.0 34 17314.0 35 22228.0 36 28865.0 37 39569.0 38 39795.0 39 25815.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 40.17553784177595 39.10551127110654 6.1369675152138505 14.58198337190366 11 27.994171595097285 34.97762921059399 16.775177852061372 20.253021342247365 12 46.77363503899888 12.32056226964944 24.133024770720837 16.772777920630837 13 22.278563469615154 15.184709008314048 27.411331104825575 35.12539641724522 14 20.51118539470301 34.19628010628268 29.906574097882917 15.385960401131396 15 41.06694094454444 14.892945915830976 27.688351761378243 16.351761378246334 16 23.321162252507072 17.798234336161826 37.342933059055454 21.537670352275647 17 38.70300848547185 14.628610611125398 22.75237850347133 23.91600239993143 18 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31 39.41494171075747 16.813979792145823 29.375646751032225 14.395431746064489 32 30.10883207537438 15.539776146347334 40.16299021714836 14.188401561129929 33 33.25329801688998 15.636469960956983 34.25362480865482 16.856607213498222 34 23.807869454696803 19.655750054176025 38.70507259587437 17.831307895252806 35 19.32180292779617 35.51036841779252 34.99384020317006 10.17398845124125 36 27.43853510533149 27.191263530197784 31.421742679142746 13.94845868532798 37 21.904227524173756 25.01987944964045 40.2580369774975 12.8178560486883 38 26.524538324015417 25.21934130437029 35.616136355024615 12.639984016589679 39 23.05934892952767 21.72199077184966 35.43051492251868 19.788145376103984 40 21.710959617380627 22.045096396219325 40.15813738573315 16.085806600666892 41 25.00750173315904 22.469329120865854 36.925427598790066 15.59774154718504 42 18.57120676270163 25.618562925903564 37.31803674631243 18.492193565082378 43 23.63894870520678 18.732400198774226 37.07864005300646 20.550011043012535 44 19.325651935360312 23.057996970230267 38.11574548553958 19.50060560886984 45 20.659505284740874 20.91459499761824 42.49021408205673 15.935685635584152 46 18.203973985446208 22.82970409135472 38.09461344085279 20.87170848234628 47 24.287312891572093 20.235340438906118 39.031146170796234 16.446200498725556 48 26.00143049158463 19.171696998386377 38.62361831443864 16.203254195590354 49 24.228589583757817 20.004351324563917 37.50082019014598 18.26623890153229 50 15.791656299245252 23.199524480937768 41.59547703961737 19.413342180199606 51 14.130119383186868 24.79031946056486 43.52477477477478 17.554786381473498 52 19.06346885660663 20.7774701631049 43.619414979106395 16.53964600118207 53 13.465885915366375 21.413855800673364 44.47897294793532 20.641285336024946 54 14.143533722819512 24.494416980675492 42.334151484772015 19.027897811732984 55 18.59972885482666 21.62036908945024 37.706817364358244 22.073084691364855 56 17.122731060907334 23.026381766184727 38.47539742095671 21.37548975195123 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 426.5 11 853.0 12 755.0 13 639.0 14 621.0 15 796.5 16 972.0 17 2111.0 18 3250.0 19 3625.0 20 4000.0 21 4309.0 22 5068.5 23 5519.0 24 6390.0 25 7261.0 26 8369.0 27 9477.0 28 10039.5 29 10626.0 30 10650.0 31 10435.5 32 10221.0 33 9342.5 34 8464.0 35 12128.0 36 15792.0 37 10699.5 38 4982.5 39 4358.0 40 4333.0 41 4308.0 42 4399.5 43 4491.0 44 5243.5 45 5996.0 46 6166.5 47 5902.5 48 5468.0 49 5472.5 50 5477.0 51 6569.5 52 7662.0 53 9010.5 54 12396.0 55 14433.0 56 14911.5 57 15390.0 58 16568.5 59 17747.0 60 18661.5 61 19576.0 62 23375.0 63 22993.5 64 18813.0 65 14227.5 66 9642.0 67 7922.5 68 6203.0 69 4706.0 70 3209.0 71 2643.5 72 1894.5 73 1711.0 74 1297.0 75 883.0 76 759.5 77 636.0 78 500.0 79 351.5 80 339.0 81 286.0 82 233.0 83 177.5 84 122.0 85 95.5 86 69.0 87 43.5 88 13.0 89 8.0 90 5.0 91 2.0 92 3.0 93 4.0 94 2.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0010285420416559527 23 0.004114168166623811 24 3.428473472186509E-4 25 0.02982771920802263 26 0.094283020485129 27 0.11211108254049885 28 0.130624839290306 29 0.17210936830376275 30 0.24959286877517783 31 0.27359218308048344 32 0.2948487186080398 33 0.3325619268020914 34 0.32776206394103025 35 0.3706179823433616 36 0.4477586354675581 37 0.40250278563469616 38 0.4710722550784263 39 0.5060426844947288 40 0.5739264592440216 41 0.5972400788548899 42 0.6342675923545041 43 0.6510671123682181 44 0.6459244021599383 45 0.6764378160623983 46 0.6826090683123339 47 0.732664781006257 48 0.7762063941030256 49 0.7227222079369161 50 0.7909488300334275 51 0.7498071483671895 52 0.8063769606582669 53 0.8163195337276078 54 0.8245478700608554 55 0.8677466358104053 56 0.85643267335219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 291675.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.55534439934475 #Duplication Level Percentage of deduplicated Percentage of total 1 72.82669231714176 25.89378126801089 2 12.1110153672288 8.612226448151533 3 4.733475344906416 5.0490103828186434 4 2.651722644366366 3.7713164748794923 5 1.569187350322044 2.7896498333897757 6 1.0950138169324626 2.3360156029844847 7 0.8314896818474358 2.069473140181102 8 0.6548104022037056 1.8625607493300964 9 0.522051566904642 1.6705550929960904 >10 2.6222360604639094 16.54584194931826 >50 0.22758653282402885 5.599394003007276 >100 0.131760624266543 9.317417174427964 >500 0.010980052022211919 3.1799816357320903 >1k 0.010980052022211919 8.067073948681966 >5k 9.981865474738107E-4 3.2357022960903423 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 9117 3.12573926459244 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 4511 1.5465843833033341 No Hit GAGATCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 2863 0.9815719550869975 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGT 2322 0.7960915402417075 No Hit GAGATCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 2293 0.7861489671723666 No Hit GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 2123 0.7278649181451958 No Hit GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1862 0.6383817605211279 No Hit GAGATCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1774 0.6082111939658866 No Hit GAGATCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1373 0.4707294077312077 No Hit GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1322 0.4532441930230565 No Hit GAGATCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1275 0.4371303677037799 No Hit GAGATCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 1012 0.3469615153852747 No Hit GAGATCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 980 0.33599040027427785 No Hit GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.32639067455215565 No Hit GAGATCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 938 0.32159081169109455 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAGCAGG 936 0.32090511699665725 No Hit GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 927 0.31781949087168937 No Hit GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 848 0.290734550441416 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 805 0.27599211451101396 No Hit GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 723 0.2478786320390846 No Hit GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 648 0.22216508099768575 No Hit GAGATCGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT 629 0.2156509814005314 No Hit GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 574 0.19679437730350563 No Hit GAGATCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 484 0.16593811605382702 No Hit GAGATCGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 424 0.145367275220708 No Hit GAGATCGGGTGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 420 0.14399588583183337 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 417 0.14296734379017742 Illumina Single End Adapter 2 (95% over 23bp) GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCCTCC 417 0.14296734379017742 No Hit GAGATCGGGGTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 411 0.14091025970686552 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 387 0.1326819233736179 No Hit GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG 382 0.13096768663752464 No Hit GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 376 0.12891060255421274 No Hit GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 370 0.12685351847090084 No Hit GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 367 0.12582497642924487 No Hit GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 346 0.1186251821376532 No Hit GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 339 0.11622525070712265 No Hit GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 336 0.1151967086654667 No Hit GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 333 0.11416816662381075 No Hit GAGATCGGGGCCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC 322 0.11039684580440559 No Hit GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 317 0.10868260906831233 No Hit GAGATCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 316 0.10833976172109369 No Hit GAGATCGGGAGCCATGTTGTTTGCCTTGCCTCAGAATTTACCTTTATGATTGGAAG 312 0.10696837233221909 No Hit GAGATCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 311 0.10662552498500043 No Hit GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 308 0.10559698294334448 No Hit GAGATCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 301 0.10319705151281393 No Hit GAGATCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 298 0.10216850947115798 No Hit GAGATCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 297 0.10182566212393931 No Hit GAGATCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 294 0.10079712008228336 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGAGATCGGGAAGCAGT 293 0.10045427273506471 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.025027856346961516 0.0 0.0 0.0 0.0 11 0.040798834319019456 0.0 0.0 0.0 0.0 12 0.04251307105511271 0.0 0.0 0.0 0.0 13 0.04457015513842462 0.0 0.0 0.0 0.0 14 0.07199794291591669 0.0 0.0 0.0 0.0 15 0.07268363761035398 0.0 0.0 0.0 0.0 16 0.0747407216936659 0.0 0.0 0.0 0.0 17 0.0781691951658524 0.0 0.0 0.0 0.0 18 0.08399760006856948 0.0 0.0 0.0 0.0 19 0.08605468415188137 0.0 0.0 0.0 0.0 20 0.10868260906831233 0.0 0.0 0.0 0.0 21 0.11828233479043455 0.0 0.0 0.0 0.0 22 0.1326819233736179 0.0 0.0 0.0 0.0 23 0.1508528327762064 0.0 0.0 0.0 0.0 24 0.1703951315676695 0.0 0.0 0.0 0.0 25 0.1731379103454187 0.0 0.0 0.0 0.0 26 0.17862346790091713 0.0 0.0 0.0 0.0 27 0.18410902545641553 0.0 0.0 0.0 0.0 28 0.2708494043027342 0.0 0.0 0.0 0.0 29 0.27736350389988856 0.0 0.0 0.0 0.0 30 0.30959115453844177 0.0 0.0 0.0 0.0 31 0.3126767806634096 0.0 0.0 0.0 0.0 32 0.31507671209394017 0.0 0.0 0.0 0.0 33 0.31850518556612667 0.0 0.0 0.0 0.0 34 0.32399074312162507 0.0 0.0 0.0 0.0 35 0.45152995628696324 0.0 0.0 0.0 0.0 36 0.45701551384246164 0.0 0.0 0.0 0.0 37 0.4621582240507414 0.0 0.0 0.0 0.0 38 0.4655866975229279 0.0 0.0 0.0 0.0 39 0.4707294077312077 0.0 0.0 0.0 0.0 40 0.4775863546755807 0.0 0.0 0.0 0.0 41 0.48410045427273507 0.0 0.0 0.0 0.0 42 0.49404302734207595 0.0 0.0 0.0 0.0 43 0.5022713636753235 0.0 0.0 0.0 0.0 44 0.5080997685780406 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCCG 45 1.2914825E-10 50.274647 50 GAGACTA 15 0.00824422 50.274643 50 TGTCCTA 15 0.0082555 50.25726 48 GGAACTC 25 2.0233394E-5 50.248573 47 GTTGTCC 15 0.008266792 50.239887 46 ACATCCC 15 0.008266792 50.239887 46 CACATCC 15 0.008272441 50.23121 45 CCACATC 15 0.008272441 50.23121 44 TTAAGCG 20 4.0846269E-4 50.231205 44 TAAGCGT 20 4.0846269E-4 50.231205 45 GCCACAT 15 0.008278094 50.222527 43 TGGTCGG 20 4.0881115E-4 50.222527 42 ACACCAT 15 0.008295069 50.19651 41 TAAGTTA 30 1.0172498E-6 50.19651 41 ACTCGGT 15 0.008295069 50.19651 41 CCGGGAT 25 2.03787E-5 50.187843 39 TCTAGTT 15 0.008300734 50.187843 39 TGACGCT 15 0.008300734 50.187843 38 TGTGATT 15 0.008300734 50.187843 40 TTGACGC 15 0.008300734 50.187843 37 >>END_MODULE