FastQCFastQC Report
Tue 31 May 2016
SRR212682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23331
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6372.7302730273027302No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4752.035917877502036No Hit
CTTCTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4271.8301830183018304No Hit
CTTCTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG3721.5944451588015944No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2581.1058248682011058No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2240.9600960096009602No Hit
CTTCTGGGGGCCTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC1360.5829154344005829No Hit
CTTCTGGGGGGTTGGTCCGAGAGTAGTGGTGTTTACAACTAATTGATCACAACCAGTT1250.5357678625005358No Hit
CTTCTGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT1220.5229094338005229No Hit
CTTCTGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1210.5186232909005186No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1200.5143371480005143No Hit
CTTCTGGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT1150.49290643350049285No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1050.45004500450045004No Hit
CTTCTGGGGGAGTCCGACTGCCGCTGTAGATCACGCGGCTGCAGCTTCCCGGACTCCG1040.44575886160044575No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1010.4329004329004329No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG980.42004200420042004No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAAGGTCTGCTAGTG900.38575286100038575No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC860.3686082894003686No Hit
CTTCTGGGGGGGCTGGAGGGATGGCTCAGCAGTTAAGAACACTCACTGATTGTTCTTC850.3643221465003643No Hit
CTTCTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT820.35146371780035146No Hit
CTTCTGGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCCT790.3386052891003386No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC750.32146071750032146No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA750.32146071750032146No Hit
CTTCTGGGGAGGCAAGGCAAATGGACTGTGGAAAAAAATAAATTCTTTTTTGTTTCCC710.3043161459003043No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT690.29574386010029574No Hit
CTTCTGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC670.28717157430028717No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT660.2828854314002829No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCGAA650.2785992885002786No Hit
CTTCTGGGGGTATCTCGGCTGCCTCGTGGTCAGGAAGCCATGGAAGGAGACTAGTGCC620.26574085980026574No Hit
CTTCTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT610.26145471690026145No Hit
CTTCTGGGGACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGGCTG590.2528824311002529No Hit
CTTCTGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG590.2528824311002529No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC570.24431014530024428No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA560.24002400240024005No Hit
CTTCTGGGGACATGTGAAAGACAAATGCACATGATGAAAACTAAAATCTTTACAAATA550.23573785950023576No Hit
CTTCTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC540.23145171660023145No Hit
CTTCTGGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCGCC510.2185932879002186No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC500.2143071450002143No Hit
CTTCTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC490.21002100210021002No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAGGGTCTGCTAGTG480.20573485920020573No Hit
CTTCTGGGGATCAGTGTGGGCCTGGAGTTACTACCTGCAGCTTCAGGACTTACCTGGG470.20144871630020145No Hit
CTTCTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA470.20144871630020145No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCTGCTCTTCCAG460.19716257340019716No Hit
CTTCTGGGGAGGGCACACCCGGTGAGGACACAGGCGTGTGTTTGGAGACATTATAAAA460.19716257340019716No Hit
CTTCTGGGGACACCCTTCGCTGACTCCACCGATCCGCCGGAACGAAAGCGGGTCCCGG450.19287643050019287No Hit
CTTCTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC450.19287643050019287No Hit
CTTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG450.19287643050019287No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.1843041447001843No Hit
CTTCTGGGGGAATGGAGAAACTCCCAAGGATCTTAGTGATGAGAAGCAGAAGAAAAAC410.17573185890017573No Hit
CTTCTGGGGATTACAATTAAATGACTTGACTGCATTTATTTTTACTTAATTTGTTCTC410.17573185890017573No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA400.17144571600017144No Hit
CTTCTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG380.16287343020016287No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT370.15858728730015859No Hit
CTTCTGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAG370.15858728730015859No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC360.1543011444001543No Hit
CTTCTGGGGGATTTCCTGGGCCTTTCAGAAATACCACGACGCTACTCAGACTACCCAG360.1543011444001543No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC360.1543011444001543No Hit
CTTCTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTGA350.15001500150015No Hit
CTTCTGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT350.15001500150015No Hit
CTTCTGGGGGGGCTAGAGAGATGGCTCAGTCGTTAAGAGCACTAACTGCTCTTCCAGA350.15001500150015No Hit
CTTCTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA350.15001500150015No Hit
CTTCTGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG350.15001500150015No Hit
CTTCTGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT350.15001500150015No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC350.15001500150015No Hit
CTTCTGGGGGCTCTTGCATCGCGGCTGCAGCTTGCTTCTGTTCCCGCGCCCGCTTGCA350.15001500150015No Hit
CTTCTGGGGAGGCTTAATGAATTCTAAGTCTATTGGTCCTGCAGAGTGATGTTGATGG350.15001500150015No Hit
CTTCTGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC340.14572885860014573No Hit
CTTCTGGGGACTACTTAATGGATGAATTGTCAAGTTCACCAAGCACAGTAATAGCCAT340.14572885860014573No Hit
CTTCTGGGGATGGAACAGAAATGTGAGAGATAATTGTGTCTTTTGTGTTTATGATAGC340.14572885860014573No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.14572885860014573No Hit
CTTCTGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT330.14144271570014144No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAATGGTTAAAGGCAGTGACTGCTCTTCCAGA330.14144271570014144No Hit
CTTCTGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA330.14144271570014144No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC320.13715657280013716No Hit
CTTCTGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC320.13715657280013716No Hit
CTTCTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA300.12858428700012858No Hit
CTTCTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC300.12858428700012858No Hit
CTTCTGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA300.12858428700012858No Hit
CTTCTGGGGGGCTGGAGAGATGGCTTAGTGGGTAAGAGCACCAACTGTTCTTCCGAAG300.12858428700012858No Hit
CTTCTGGGGGGTCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA290.12429814410012428No Hit
CTTCTGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA290.12429814410012428No Hit
CTTCTGGGGGAGTCCTTTTTAAAAGATAACTGCCAGCTGCAGGCATCTGGCTCTGCAA290.12429814410012428No Hit
CTTCTGGGGGGGCGGTGGTGGCACACGCCTTTAATCCCGGCACTTGGAAGGCAGAGGC270.11572585830011572No Hit
CTTCTGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT270.11572585830011572No Hit
CTTCTGGGGGTCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA270.11572585830011572No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCGA260.11143971540011144No Hit
CTTCTGGGGGGCTTTGCTGCTGGCGCCTTGCGGGAAGTCGCTCGAGGAAGCCATGGAG260.11143971540011144No Hit
CTTCTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG260.11143971540011144No Hit
CTTCTGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG260.11143971540011144No Hit
CTTCTGGGGGCCTGAGAGATGGCTCAGTGGTTAATAGTACTGACTGCTCTTCCAAAGG260.11143971540011144No Hit
CTTCTGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA260.11143971540011144No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC260.11143971540011144No Hit
CTTCTGGGGCTCACAACAACTACCAGGCCCAGTCCGCTGTTCCCCTGCGCCATGAGAC250.10715357250010715No Hit
CTTCTGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC240.10286742960010287No Hit
CTTCTGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTG240.10286742960010287No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA240.10286742960010287No Hit
CTTCTGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.10286742960010287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC203.3779602E-452.00000426
ACTAATT203.3779602E-452.00000438
ACTGCCG203.3779602E-452.00000417
CAATGGG203.3779602E-452.00000428
TCCTTCC203.3779602E-452.00000414
AGCTTCC203.3779602E-452.00000443
TAGTGGT203.3779602E-452.00000424
TAGTGAG203.3779602E-452.00000415
TGGTATC353.6676283E-852.00000416
AGTAGTG203.3779602E-452.00000422
GGGTATC203.3779602E-452.0000048
CACGCGG203.3779602E-452.00000432
GGTCCGA203.3779602E-452.00000414
CAACTAA203.3779602E-452.00000436
CACAACC203.3779602E-452.00000448
GTCCGAG203.3779602E-452.00000415
GTCCGAC203.3779602E-452.00000412
TCGAACT203.3779602E-452.00000452
TGATCAC203.3779602E-452.00000444
TGTTTAC203.3779602E-452.00000430
Produced by FastQC (version 0.11.5)