FastQCFastQC Report
Tue 31 May 2016
SRR212681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25498
Sequences flagged as poor quality0
Sequence length58
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7282.8551258922268414No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5382.1099694093654406No Hit
CTTCTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4251.6667973958741862No Hit
CTTCTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4081.600125500039219No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3211.2589222684132089No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2170.8510471409522316No Hit
CTTCTGGGGGCCTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC1560.611812691191466No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1490.5843595576123618No Hit
CTTCTGGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT1450.5686720527100165No Hit
CTTCTGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT1370.537297042905326No Hit
CTTCTGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1330.5216095380029807No Hit
CTTCTGGGGGAGTCCGACTGCCGCTGTAGATCACGCGGCTGCAGCTTCCCGGACTCCG1260.49415640442387637No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1130.44317201349125424No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1130.44317201349125424No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1110.4353282610400816No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAAGGTCTGCTAGTG1100.4314063848144953No Hit
CTTCTGGGGGGTTGGTCCGAGAGTAGTGGTGTTTACAACTAATTGATCACAACCAGTT1030.40395325123539105No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA950.37257824143070045No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCGAA900.35296886030276886No Hit
CTTCTGGGGAGGCAAGGCAAATGGACTGTGGAAAAAAATAAATTCTTTTTTGTTTCCC880.3451251078515962No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC860.3372813554004236No Hit
CTTCTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT810.31767197427249194No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC800.3137500980469056No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT780.305906345595733No Hit
CTTCTGGGGGGGCTGGAGGGATGGCTCAGCAGTTAAGAACACTCACTGATTGTTCTTC760.29806259314456035No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC720.2823750882422151No Hit
CTTCTGGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCGCC700.27453133579104244No Hit
CTTCTGGGGACATGTGAAAGACAAATGCACATGATGAAAACTAAAATCTTTACAAATA690.2706094595654561No Hit
CTTCTGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC650.2549219546631108No Hit
CTTCTGGGGGTATCTCGGCTGCCTCGTGGTCAGGAAGCCATGGAAGGAGACTAGTGCC650.2549219546631108No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT640.2510000784375245No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA640.2510000784375245No Hit
CTTCTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC630.24707820221193819No Hit
CTTCTGGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCCT610.23923444976076555No Hit
CTTCTGGGGATCAGTGTGGGCCTGGAGTTACTACCTGCAGCTTCAGGACTTACCTGGG610.23923444976076555No Hit
CTTCTGGGGAGGGCACACCCGGTGAGGACACAGGCGTGTGTTTGGAGACATTATAAAA560.21962506863283396No Hit
CTTCTGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG530.207859439956075No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA520.20393756373048869No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC470.18432818260255707No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCTGCTCTTCCAG470.18432818260255707No Hit
CTTCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG470.18432818260255707No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAGGGTCTGCTAGTG470.18432818260255707No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC470.18432818260255707No Hit
CTTCTGGGGGCTCTTGCATCGCGGCTGCAGCTTGCTTCTGTTCCCGCGCCCGCTTGCA470.18432818260255707No Hit
CTTCTGGGGACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGGCTG460.18040630637697075No Hit
CTTCTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA450.17648443015138443No Hit
CTTCTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC450.17648443015138443No Hit
CTTCTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA450.17648443015138443No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.1725625539257981No Hit
CTTCTGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA440.1725625539257981No Hit
CTTCTGGGGACACCCTTCGCTGACTCCACCGATCCGCCGGAACGAAAGCGGGTCCCGG430.1686406777002118No Hit
CTTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG420.16471880147462545No Hit
CTTCTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT410.16079692524903913No Hit
CTTCTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC400.1568750490234528No Hit
CTTCTGGGGGATCTCCAGGCGGCTTTTGGGCTCCAGAGGCTGTGTGCTCGCCGAGGTT390.1529531727978665No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC390.1529531727978665No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC390.1529531727978665No Hit
CTTCTGGGGGTCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA390.1529531727978665No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCG380.14903129657228018No Hit
CTTCTGGGGGAATGGAGAAACTCCCAAGGATCTTAGTGATGAGAAGCAGAAGAAAAAC380.14903129657228018No Hit
CTTCTGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAG370.14510942034669386No Hit
CTTCTGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC360.14118754412110754No Hit
CTTCTGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT360.14118754412110754No Hit
CTTCTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG360.14118754412110754No Hit
CTTCTGGGGATTACAATTAAATGACTTGACTGCATTTATTTTTACTTAATTTGTTCTC360.14118754412110754No Hit
CTTCTGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT350.13726566789552122No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC350.13726566789552122No Hit
CTTCTGGGGCTCACAACAACTACCAGGCCCAGTCCGCTGTTCCCCTGCGCCATGAGAC350.13726566789552122No Hit
CTTCTGGGGGATTTCCTGGGCCTTTCAGAAATACCACGACGCTACTCAGACTACCCAG340.1333437916699349No Hit
CTTCTGGGGGGTCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA340.1333437916699349No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT340.1333437916699349No Hit
CTTCTGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC330.12942191544434856No Hit
CTTCTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA320.12550003921876224No Hit
CTTCTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTGA320.12550003921876224No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC320.12550003921876224No Hit
CTTCTGGGGGGCTGGAGAGATGGCTTAGTGGGTAAGAGCACCAACTGTTCTTCCGAAG310.12157816299317593No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA310.12157816299317593No Hit
CTTCTGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT300.11765628676758962No Hit
CTTCTGGGGGGGCGGTGGTGGCACACGCCTTTAATCCCGGCACTTGGAAGGCAGAGGC300.11765628676758962No Hit
CTTCTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC300.11765628676758962No Hit
CTTCTGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA300.11765628676758962No Hit
CTTCTGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC300.11765628676758962No Hit
CTTCTGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT280.10981253431641698No Hit
CTTCTGGGGGAGTCCTTTTTAAAAGATAACTGCCAGCTGCAGGCATCTGGCTCTGCAA280.10981253431641698No Hit
CTTCTGGGGGCCTGAGAGATGGCTCAGTGGTTAATAGTACTGACTGCTCTTCCAAAGG270.10589065809083066No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA270.10589065809083066No Hit
CTTCTGGGGGGGCTAGAGAGATGGCTCAGTCGTTAAGAGCACTAACTGCTCTTCCAGA260.10196878186524434No Hit
CTTCTGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA260.10196878186524434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCC150.007056466352.15384752
AACCAGT150.007056466352.15384751
CCCCGTC203.3346884E-452.15384750
TCATGGG150.007056466352.15384750
CATGGGC150.007056466352.15384751
ATCGAAC401.7262209E-952.15384752
ACCAGTT150.007056466352.15384752
TCCCCGT203.3346884E-452.15384749
TTTGATC150.007056466352.15384749
TGATGAA150.00711182652.0511832
ATCCTAT150.00711182652.0511838
ACTAATT150.00711182652.0511838
TTGTCAT150.00711182652.0511844
CCTATTG150.00711182652.0511840
GTTTGAT150.00711182652.0511848
TCCTATT150.00711182652.0511839
GAGGACT150.00711182652.0511837
AGGACTG150.00711182652.0511838
CGCCTCG150.00711182652.0511845
ATGATGA150.00711182652.0511831
Produced by FastQC (version 0.11.5)