FastQCFastQC Report
Tue 31 May 2016
SRR212680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27004
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7062.614427492223374No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5371.988594282328544No Hit
CTTCTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4471.6553103243963856No Hit
CTTCTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG4201.5553251370167382No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3161.1701970078506887No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2260.8369130499185307No Hit
CTTCTGGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT1590.5888016590134795No Hit
CTTCTGGGGGCCTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC1460.5406606428677233No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1330.49251962672196714No Hit
CTTCTGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGT1310.4851133165456969No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1280.47400385128129163No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAAGGTCTGCTAGTG1160.4295659902236706No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1100.40734705969486No Hit
CTTCTGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1060.3925344393423197No Hit
CTTCTGGGGGAGTCCGACTGCCGCTGTAGATCACGCGGCTGCAGCTTCCCGGACTCCG980.3629091986372389No Hit
CTTCTGGGGGGTTGGTCCGAGAGTAGTGGTGTTTACAACTAATTGATCACAACCAGTT940.34809657828469853No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG920.3406902681084284No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA910.3369871130202933No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT900.3332839579321582No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC880.325877647755888No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC840.3110650274033476No Hit
CTTCTGGGGACATGTGAAAGACAAATGCACATGATGAAAACTAAAATCTTTACAAATA750.2777366316101318No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC750.2777366316101318No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCGAA740.2740334765219967No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC720.2666271663457266No Hit
CTTCTGGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCGCC710.26292401125759146No Hit
CTTCTGGGGGGGCTGGAGGGATGGCTCAGCAGTTAAGAACACTCACTGATTGTTCTTC710.26292401125759146No Hit
CTTCTGGGGAGGCAAGGCAAATGGACTGTGGAAAAAAATAAATTCTTTTTTGTTTCCC700.25922085616945634No Hit
CTTCTGGGGACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGGCTG670.2481113909050511No Hit
CTTCTGGGGATCAGTGTGGGCCTGGAGTTACTACCTGCAGCTTCAGGACTTACCTGGG650.2407050807287809No Hit
CTTCTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC630.23329877055251075No Hit
CTTCTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC630.23329877055251075No Hit
CTTCTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT610.22589246037624058No Hit
CTTCTGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC600.22218930528810546No Hit
CTTCTGGGGAGGGCACACCCGGTGAGGACACAGGCGTGTGTTTGGAGACATTATAAAA600.22218930528810546No Hit
CTTCTGGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCCT590.21848615019997036No Hit
CTTCTGGGGGTATCTCGGCTGCCTCGTGGTCAGGAAGCCATGGAAGGAGACTAGTGCC580.2147829951118353No Hit
CTTCTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT540.1999703747592949No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA530.19626721967115984No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT510.18886090949488965No Hit
CTTCTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA500.18515775440675455No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCTGCTCTTCCAG470.17404828914234927No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC440.162938823877944No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAGGGTCTGCTAGTG440.162938823877944No Hit
CTTCTGGGGGTCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA440.162938823877944No Hit
CTTCTGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG430.15923566878980894No Hit
CTTCTGGGGACACCCTTCGCTGACTCCACCGATCCGCCGGAACGAAAGCGGGTCCCGG420.1555325137016738No Hit
CTTCTGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT420.1555325137016738No Hit
CTTCTGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAG410.15182935861353875No Hit
CTTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG410.15182935861353875No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.14812620352540362No Hit
CTTCTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC400.14812620352540362No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA390.14442304843726855No Hit
CTTCTGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA390.14442304843726855No Hit
CTTCTGGGGATTACAATTAAATGACTTGACTGCATTTATTTTTACTTAATTTGTTCTC380.14071989334913348No Hit
CTTCTGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT370.13701673826099836No Hit
CTTCTGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC360.1333135831728633No Hit
CTTCTGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT350.12961042808472817No Hit
CTTCTGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA350.12961042808472817No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC340.1259072729965931No Hit
CTTCTGGGGGCTCTTGCATCGCGGCTGCAGCTTGCTTCTGTTCCCGCGCCCGCTTGCA340.1259072729965931No Hit
CTTCTGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGAC330.12220411790845802No Hit
CTTCTGGGGGGGCTAGAGAGATGGCTCAGTCGTTAAGAGCACTAACTGCTCTTCCAGA330.12220411790845802No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAATGGTTAAAGGCAGTGACTGCTCTTCCAGA330.12220411790845802No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC330.12220411790845802No Hit
CTTCTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG330.12220411790845802No Hit
CTTCTGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC320.11850096282032291No Hit
CTTCTGGGGGATTTCCTGGGCCTTTCAGAAATACCACGACGCTACTCAGACTACCCAG320.11850096282032291No Hit
CTTCTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTGA320.11850096282032291No Hit
CTTCTGGGGGGCTGGAGAGATGGCTTAGTGGGTAAGAGCACCAACTGTTCTTCCGAAG320.11850096282032291No Hit
CTTCTGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG320.11850096282032291No Hit
CTTCTGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT310.11479780773218783No Hit
CTTCTGGGGGATCTCCAGGCGGCTTTTGGGCTCCAGAGGCTGTGTGCTCGCCGAGGTT310.11479780773218783No Hit
CTTCTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC310.11479780773218783No Hit
CTTCTGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC300.11109465264405273No Hit
CTTCTGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA300.11109465264405273No Hit
CTTCTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA290.10739149755591765No Hit
CTTCTGGGGATGGAACAGAAATGTGAGAGATAATTGTGTCTTTTGTGTTTATGATAGC290.10739149755591765No Hit
CTTCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG280.10368834246778254No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT280.10368834246778254No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCGAA280.10368834246778254No Hit
CTTCTGGGGGAATGGAGAAACTCCCAAGGATCTTAGTGATGAGAAGCAGAAGAAAAAC280.10368834246778254No Hit
CTTCTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG280.10368834246778254No Hit
CTTCTGGGGAGGCTTAATGAATTCTAAGTCTATTGGTCCTGCAGAGTGATGTTGATGG280.10368834246778254No Hit
CTTCTGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA280.10368834246778254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCAA150.00704370752.186229
GATGCCA150.00704370752.186229
GGATCAA150.00704370752.186228
ACCAAGG150.00704370752.1862210
TGGGGCG251.5781914E-552.186225
TGGGGCA650.052.186225
CCATCTC150.00704370752.1862210
ATGCCAC150.00704370752.1862210
GGGACAC150.00704370752.186227
AGGCAAG150.00704370752.1862210
GGGGTTT150.00704370752.186228
GGGCAGA203.3280582E-452.1862148
TGGGGTC203.3280582E-452.1862145
TGGGGTA203.3280582E-452.1862145
GTTTCCC150.007095862652.0892252
CCGTCAT203.3588795E-452.0892252
CCCGTCA251.5957468E-552.0892251
AAGATGC203.3588795E-452.0892252
TGCTAGT307.614126E-752.0892251
CTGCTAG307.614126E-752.0892250
Produced by FastQC (version 0.11.5)