FastQCFastQC Report
Tue 31 May 2016
SRR212679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55392
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC15712.836149624494512No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10121.8269786250722126No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC9721.7547660311958406No Hit
CTTCTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8621.5561813980358175No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7881.4225880993645292No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7851.4171721548238012No Hit
CTTCTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA6441.1626227614095899No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3840.6932409012131715No Hit
CTTCTGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3490.630054881571346No Hit
CTTCTGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC3440.6210283073367995No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAAGGTCTGCTAG3080.5560369728480647No Hit
CTTCTGGGGGCCTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT3050.5506210283073367No Hit
CTTCTGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC2870.5181253610629694No Hit
CTTCTGGGGGGTTGGTCCGAGAGTAGTGGTGTTTACAACTAATTGATCACAACCAG2270.4098064702484114No Hit
CTTCTGGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC2210.3989745811669555No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2020.3646735990756788No Hit
CTTCTGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC1720.31051415366839974No Hit
CTTCTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1650.29787694974003465No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1630.29426632004621606No Hit
CTTCTGGGGAGGCAAGGCAAATGGACTGTGGAAAAAAATAAATTCTTTTTTGTTTC1630.29426632004621606No Hit
CTTCTGGGGGGGCTGGAGGGATGGCTCAGCAGTTAAGAACACTCACTGATTGTTCT1550.27982380127094164No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.26177065280184864No Hit
CTTCTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1450.26177065280184864No Hit
CTTCTGGGGGAATGGAGAAACTCCCAAGGATCTTAGTGATGAGAAGCAGAAGAAAA1400.25274407856730213No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1340.2419121894858463No Hit
CTTCTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1330.240106874638937No Hit
CTTCTGGGGAGGGCACACCCGGTGAGGACACAGGCGTGTGTTTGGAGACATTATAA1270.22927498555748121No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCG1260.22746967071057192No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCTGCTCTTCC1250.22566435586366262No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAGGGTCTGCTAG1210.21844309647602542No Hit
CTTCTGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1190.2148324667822068No Hit
CTTCTGGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCG1170.2112218370883882No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1120.2021952628538417No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1070.1931686886192952No Hit
CTTCTGGGGGTATCTCGGCTGCCTCGTGGTCAGGAAGCCATGGAAGGAGACTAGTG1060.1913633737723859No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1030.185947429231658No Hit
CTTCTGGGGATCAGTGTGGGCCTGGAGTTACTACCTGCAGCTTCAGGACTTACCTG1020.1841421143847487No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1010.1823367995378394No Hit
CTTCTGGGGGAAGAAGGGAGACAGTTCTGTAAGTCCTGCGGGGCGGCAGAGGTCCG990.17872616984402082No Hit
CTTCTGGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTC980.17692085499711152No Hit
CTTCTGGGGATTACAATTAAATGACTTGACTGCATTTATTTTTACTTAATTTGTTC930.167894280762565No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC910.16428365106874637No Hit
CTTCTGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG900.16247833622183708No Hit
CTTCTGGGGGAACTACAAGCCTCCAGCCCAGAAGAGCATCCAGGAGATCCAGGAAC880.1588677065280185No Hit
CTTCTGGGGATGGGGGGTTGTATTTATAGGGTGAGGAGGGACTAGATGTCCGAGGA870.1570623916811092No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTTCTGGGGAAGCAGT870.1570623916811092No Hit
CTTCTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA860.15525707683419987No Hit
CTTCTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG850.15345176198729057No Hit
CTTCTGGGGAAGTTTTTTGTTGTTGGTGGTAGTGGTTTTTTATATATATATATATA840.15164644714038128No Hit
CTTCTGGGGGGTCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT840.15164644714038128No Hit
CTTCTGGGGGTCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG820.1480358174465627No Hit
CTTCTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC810.1462305025996534No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC810.1462305025996534No Hit
CTTCTGGGGACATGTGAAAGACAAATGCACATGATGAAAACTAAAATCTTTACAAA750.13539861351819757No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAATGGTTAAAGGCAGTGACTGCTCTTCCA730.13178798382437898No Hit
CTTCTGGGGGGCTGGAGAGATGGCTTAGTGGGTAAGAGCACCAACTGTTCTTCCGA730.13178798382437898No Hit
CTTCTGGGGACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGGC710.1281773541305604No Hit
CTTCTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA710.1281773541305604No Hit
CTTCTGGGGAGGCTTAATGAATTCTAAGTCTATTGGTCCTGCAGAGTGATGTTGAT700.12637203928365107No Hit
CTTCTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT690.12456672443674176No Hit
CTTCTGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC670.12095609474292315No Hit
CTTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG660.11915077989601386No Hit
CTTCTGGGGATCTTTGAAGTTAGGAGACTTTTCATTACAAGATGTGCTTTGCCATA650.11734546504910456No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC640.11554015020219525No Hit
CTTCTGGGGATGGAACAGAAATGTGAGAGATAATTGTGTCTTTTGTGTTTATGATA640.11554015020219525No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTTCTGGGGAAGCAGG640.11554015020219525No Hit
CTTCTGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC630.11373483535528596No Hit
CTTCTGGGGGGGCTAGAGAGATGGCTCAGTCGTTAAGAGCACTAACTGCTCTTCCA610.11012420566146736No Hit
CTTCTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC610.11012420566146736No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT600.10831889081455806No Hit
CTTCTGGGGGAGTCCGACTGCCGCTGTAGATCACGCGGCTGCAGCTTCCCGGACTC590.10651357596764875No Hit
CTTCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.10470826112073946No Hit
CTTCTGGGGACACCCTTCGCTGACTCCACCGATCCGCCGGAACGAAAGCGGGTCCC580.10470826112073946No Hit
CTTCTGGGGACTACTTAATGGATGAATTGTCAAGTTCACCAAGCACAGTAATAGCC580.10470826112073946No Hit
CTTCTGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC580.10470826112073946No Hit
CTTCTGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG570.10290294627383015No Hit
CTTCTGGGGGGGCGGTGGTGGCACACGCCTTTAATCCCGGCACTTGGAAGGCAGAG570.10290294627383015No Hit
CTTCTGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGA570.10290294627383015No Hit
CTTCTGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG570.10290294627383015No Hit
CTTCTGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC560.10109763142692085No Hit
CTTCTGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA560.10109763142692085No Hit
CTTCTGGGGGTGCGTGCTTAGGACCACCGTGGCAGCTTGAGGGCGGCCTAGAGGCC560.10109763142692085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGT402.4829205E-950.26800543
GTTCTCG204.0408707E-450.26800550
GATAAGG402.4829205E-950.26800542
GTCGAGA204.0408707E-450.26800543
CGAGGAA204.0408707E-450.26800543
TCTGCTA550.050.26800549
AACCAGT204.0408707E-450.26800545
AAGGCCT204.0408707E-450.26800549
AAGGTCT402.4829205E-950.26800545
CTGCTAG550.050.26800550
GCACAGT204.0408707E-450.26800543
TCGAGGA204.0408707E-450.26800542
TCGAGAT204.0408707E-450.26800544
TAAGGTC402.4829205E-950.26800544
TGTCGAG204.0408707E-450.26800542
GGACGCG204.0408707E-450.26800550
ACAGTGT150.00821315550.26844
AGAAGTG150.00821315550.26842
CCAGTTC251.9971758E-550.26847
AATTTGT150.00821315550.26848
Produced by FastQC (version 0.11.5)