FastQCFastQC Report
Tue 31 May 2016
SRR212678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58151
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16872.9010679094082645No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11481.974170693539234No Hit
CTTCTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9251.590686316658355No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9091.5631717425323728No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8821.5167408986947775No Hit
CTTCTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA7691.322419218930027No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7361.2656704097951883No Hit
CTTCTGGGGGCCTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT4220.7256968925727847No Hit
CTTCTGGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3940.6775463878523155No Hit
CTTCTGGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC3860.6637891007893243No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3650.6276762222489726No Hit
CTTCTGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC3410.586404361059999No Hit
CTTCTGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC3390.5829650392942511No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAAGGTCTGCTAG2850.49010335161906077No Hit
CTTCTGGGGGGTTGGTCCGAGAGTAGTGGTGTTTACAACTAATTGATCACAACCAG2560.4402331860157177No Hit
CTTCTGGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC2510.43163488160134816No Hit
CTTCTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2430.417877594538357No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2090.3594091245206445No Hit
CTTCTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2010.3456518374576533No Hit
CTTCTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1980.34049285480903163No Hit
CTTCTGGGGGAATGGAGAAACTCCCAAGGATCTTAGTGATGAGAAGCAGAAGAAAA1900.32673556774604046No Hit
CTTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1630.28030472390844524No Hit
CTTCTGGGGAGGCAAGGCAAATGGACTGTGGAAAAAAATAAATTCTTTTTTGTTTC1590.27342608037694965No Hit
CTTCTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1550.26654743684545407No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.2596687933139585No Hit
CTTCTGGGGGTGCGTGCTTAGGACCACCGTGGCAGCTTGAGGGCGGCCTAGAGGCC1480.2545098106653368No Hit
CTTCTGGGGGGGCTGGAGGGATGGCTCAGCAGTTAAGAACACTCACTGATTGTTCT1460.251070488899589No Hit
CTTCTGGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCG1400.24075252360234561No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1280.22011659300785885No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCG1260.21667727124211103No Hit
CTTCTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGTCTGCTCTTCC1250.21495761035923716No Hit
CTTCTGGGGGAACTACAAGCCTCCAGCCCAGAAGAGCATCCAGGAGATCCAGGAAC1160.1994806624133721No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1150.1977610015304982No Hit
CTTCTGGGGGTATCTCGGCTGCCTCGTGGTCAGGAAGCCATGGAAGGAGACTAGTG1110.19088235799900258No Hit
CTTCTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1090.18744303623325478No Hit
CTTCTGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1070.184003714467507No Hit
CTTCTGGGGGAAGAAGGGAGACAGTTCTGTAAGTCCTGCGGGGCGGCAGAGGTCCG1070.184003714467507No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1060.18228405358463312No Hit
CTTCTGGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTC1040.17884473181888533No Hit
CTTCTGGGGATTACAATTAAATGACTTGACTGCATTTATTTTTACTTAATTTGTTC1040.17884473181888533No Hit
CTTCTGGGGGACATAGGGTGTTGAGGTATTGCAGTATTCATGATAGGGTCTGCTAG1030.1771250709360114No Hit
CTTCTGGGGATGGGGGGTTGTATTTATAGGGTGAGGAGGGACTAGATGTCCGAGGA1010.1736857491702636No Hit
CTTCTGGGGGAGTCCGACTGCCGCTGTAGATCACGCGGCTGCAGCTTCCCGGACTC990.17024642740451582No Hit
CTTCTGGGGAGGGCACACCCGGTGAGGACACAGGCGTGTGTTTGGAGACATTATAA980.16852676652164195No Hit
CTTCTGGGGACACCCTTCGCTGACTCCACCGATCCGCCGGAACGAAAGCGGGTCCC980.16852676652164195No Hit
CTTCTGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG980.16852676652164195No Hit
CTTCTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC970.16680710563876802No Hit
CTTCTGGGGACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGACAGTGTGGTGGC940.16164812299014633No Hit
CTTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG930.15992846210727243No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC900.15476947945865074No Hit
CTTCTGGGGATCAGTGTGGGCCTGGAGTTACTACCTGCAGCTTCAGGACTTACCTG900.15476947945865074No Hit
CTTCTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC880.15133015769290295No Hit
CTTCTGGGGGTCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG860.14789083592715516No Hit
CTTCTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA840.14445151416140736No Hit
CTTCTGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC830.14273185327853347No Hit
CTTCTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA820.14101219239565957No Hit
CTTCTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA820.14101219239565957No Hit
CTTCTGGGGATGGAACAGAAATGTGAGAGATAATTGTGTCTTTTGTGTTTATGATA800.13757287062991178No Hit
CTTCTGGGGGGCTGGAGAGATGGCTTAGTGGGTAAGAGCACCAACTGTTCTTCCGA790.13585320974703788No Hit
CTTCTGGGGGATCTCCAGGCGGCTTTTGGGCTCCAGAGGCTGTGTGCTCGCCGAGG780.13413354886416398No Hit
CTTCTGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT780.13413354886416398No Hit
CTTCTGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG700.12037626180117281No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC690.1186566009182989No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTTCTGGGGAAGCAGT690.1186566009182989No Hit
CTTCTGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC690.1186566009182989No Hit
CTTCTGGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGA680.11693694003542501No Hit
CTTCTGGGGACATGTGAAAGACAAATGCACATGATGAAAACTAAAATCTTTACAAA670.1152172791525511No Hit
CTTCTGGGGAAGTTTTTTGTTGTTGGTGGTAGTGGTTTTTTATATATATATATATA670.1152172791525511No Hit
CTTCTGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG670.1152172791525511No Hit
CTTCTGGGGGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGG660.11349761826967722No Hit
CTTCTGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA650.11177795738680331No Hit
CTTCTGGGGCTCACAACAACTACCAGGCCCAGTCCGCTGTTCCCCTGCGCCATGAG630.10833863562105552No Hit
CTTCTGGGGGCTTTTTAGTTAGTGCTGGAACGTGGAGGAAGCTGCTCCCTCCGAAG620.10661897473818163No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT620.10661897473818163No Hit
CTTCTGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC620.10661897473818163No Hit
CTTCTGGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC610.10489931385530772No Hit
CTTCTGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC610.10489931385530772No Hit
CTTCTGGGGAGGCTTAATGAATTCTAAGTCTATTGGTCCTGCAGAGTGATGTTGAT610.10489931385530772No Hit
CTTCTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC590.10145999208955993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTC204.013337E-450.34201450
AGAAACA550.050.34201450
ACTAGTG204.013337E-450.34201450
CCCCTAT204.013337E-450.34201450
CATCGAA204.013337E-450.34201450
AGGTCCG150.00816760250.3420150
CAGACCG150.00816760250.3420150
CGCACTC150.00816760250.3420150
TCTTCGG150.00816760250.3420150
AAGTTCA150.00816760250.3420150
TCCCCTA204.0305854E-450.29835549
GACTAGT204.0305854E-450.29835549
TCTGCTA309.8907E-750.2983549
GCGCACT150.00819569750.2983549
TCCCCGT309.8907E-750.2983549
ATCCCCT204.065259E-450.21125848
TGATCAC204.065259E-450.21125844
ACATCCC204.065259E-450.21125846
AGGTCTG204.065259E-450.21125846
GATCACA204.065259E-450.21125845