FastQCFastQC Report
Tue 31 May 2016
SRR212677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144344
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT45853.1764396164717623No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT23801.6488388848861053No Hit
ATACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG23481.626669622568309No Hit
ATACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC22771.5774815718006985No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC22621.5670897300892315No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC18691.2948234772487945No Hit
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT17431.2075320068724713No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15781.0932217480463338No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA13010.9013190711079089No Hit
ATACAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT12200.8452031258659868No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA11240.7786953389125977No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9610.6657706589813224No Hit
ATACAGGGGGTTTTAATCATGGGCTTGGTATGTTAGTAACTGTTTTTATATTCACTTC9070.628360028820041No Hit
ATACAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG8170.5660089785512387No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTTAGTGGTTAAGAGCACTGACTGCTCTTCCAA8120.562545031314083No Hit
ATACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6960.4821814554120712No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA6560.4544698775148257No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.37757024884996954No Hit
ATACAGGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5160.3574793548744665No Hit
ATACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5030.34847309205786176No Hit
ATACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT4970.344316355373275No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC4900.339466829241257No Hit
ATACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4770.3304605664246522No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC4420.30621293576456243No Hit
ATACAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC4350.3013634096325445No Hit
ATACAGGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCTGA4040.27988693676217924No Hit
ATACAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4020.27850135786731695No Hit
ATACAGGGGGTCTCAGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCAAG3860.2674167267084188No Hit
ATACAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCCAA3850.26672393726098764No Hit
ATACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3610.25009699052264034No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCAAA3550.24594025383805354No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG3540.2452474643906224No Hit
ATACAGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3500.24247630660089786No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3490.24178351715346671No Hit
ATACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3430.23762678046887992No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCCAA3390.23485562267915533No Hit
ATACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA3290.22792772820484397No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG3270.2265421493099817No Hit
ATACAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC3240.22446378096768832No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCAGA3220.22307820207282605No Hit
ATACAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA3050.2113007814664967No Hit
ATACAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2880.19952336086016736No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2830.1960594136230117No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC2700.18705315080640691No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCGAA2660.18428199301668235No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA2630.18220362467438897No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA2500.1731973618577842No Hit
ATACAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG2350.16280552014631713No Hit
ATACAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2330.16141994125145484No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG2320.16072715180402372No Hit
ATACAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2280.1579559940142992No Hit
ATACAGGGGGGGTGCTGGAGGAATGGCTTAGTGGTTAAGAGTACTAATTGTTCTTCCA2280.1579559940142992No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT2270.15726320456686804No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCGA2150.14894973119769442No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAAA2140.14825694175026324No Hit
ATACAGGGGGGGCTGGAGAGATGGCTTGGCGGTTAAGAGCACTGGCTGCTCTTCCGAA2140.14825694175026324No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCAACTGCTCTTCCG2130.14756415230283212No Hit
ATACAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA2060.14271462617081418No Hit
ATACAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2000.13855788948622735No Hit
ATACAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1990.1378651000387962No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA1960.1357867316965028No Hit
ATACAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA1850.1281660477747603No Hit
ATACAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1830.12678046887989802No Hit
ATACAGGGGGGGCTGTTGAGATGGCTCAGTGGGTAAGAGCACCCGAGTGCTCTTCCAA1780.12331652164274233No Hit
ATACAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1760.12193094274788006No Hit
ATACAGGGGAGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1750.12123815330044893No Hit
ATACAGGGGGGCTCCTCAACCACACCACACCACATCCCCATTATACCCAAGACTTGCA1750.12123815330044893No Hit
ATACAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1730.11985257440558666No Hit
ATACAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCAATGACTGCTCTTCTGA1730.11985257440558666No Hit
ATACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.11777420606329324No Hit
ATACAGGGGGATTGAATGTGGGCTTTATGCAGACCTCTAGGAGGGGCAGATAGATGGT1680.11638862716843096No Hit
ATACAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1650.11431025882613756No Hit
ATACAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAAG1630.11292467993127529No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1630.11292467993127529No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGTGCCGACTGCTCTTCCAAA1610.11153910103641303No Hit
ATACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA1570.10876794324668845No Hit
ATACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTGA1510.10461120656210166No Hit
ATACAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC1480.10253283821980823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGCA203.425238E-452.0378152
ATTAAGC203.425238E-452.0378151
AATGAAG203.425238E-452.0378151
GTATGGG203.425238E-452.0378152
TTAAGCC203.425238E-452.0378152
AGATCTT150.007186718352.03780752
GATAAGG150.007186718352.03780751
AGCCCAA251.6408527E-552.03780752
ATGTGTG150.007186718352.03780751
TATGGGT150.007186718352.03780752
TTCACTT700.052.03780751
GCGCACT150.007186718352.03780751
TCGTCGT150.007186718352.03780752
GCCTAAA251.6408527E-552.03780752
TAGATGG251.6408527E-552.03780751
TCTTTCA251.6408527E-552.03780751
CGACTGA150.007186718352.03780752
AGCTAAG150.007186718352.03780752
TCGAACT353.8297003E-852.03780752
CAGATAG251.6442267E-552.01976847
Produced by FastQC (version 0.11.5)