FastQCFastQC Report
Tue 31 May 2016
SRR212676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143064
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT44003.0755466085108765No Hit
ATACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG22751.5901973941732372No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT22261.555946988760275No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC22051.5412682435832914No Hit
ATACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC21991.5370743163898675No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC18461.2903315998434268No Hit
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT16641.1631158083095676No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15371.0743443493820948No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA12330.861852038248616No Hit
ATACAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT11190.7821674215735614No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA10490.7332382709836157No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9450.6605435329642677No Hit
ATACAGGGGGTTTTAATCATGGGCTTGGTATGTTAGTAACTGTTTTTATATTCACTTC8450.5906447464072023No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTTAGTGGTTAAGAGCACTGACTGCTCTTCCAA8260.5773639769613599No Hit
ATACAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG7110.4969803724207348No Hit
ATACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6960.48649555443717496No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA6410.448051221830789No Hit
ATACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT5390.37675445954258235No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.3760554716770117No Hit
ATACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC5060.35368785997875074No Hit
ATACAGGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4640.32433036962478334No Hit
ATACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4570.3194374545657887No Hit
ATACAGGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCTGA4260.2977688307330985No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC4220.2949728792708159No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC4170.29147793994296256No Hit
ATACAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC3920.27400324330369624No Hit
ATACAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCCAA3770.2635184253201364No Hit
ATACAGGGGGTCTCAGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCAAG3720.26002348599228314No Hit
ATACAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3700.25862551026114183No Hit
ATACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3620.25303360733657665No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG3610.252334619471006No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3400.23765587429402227No Hit
ATACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3380.23625789856288096No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG3350.234160934966169No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCAAA3350.234160934966169No Hit
ATACAGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3340.23346194710059834No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCCAA3120.21808421405804393No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCAGA3070.21458927473019068No Hit
ATACAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2900.20270648101548958No Hit
ATACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2870.2006095174187776No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCGAA2700.1887267237040765No Hit
ATACAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC2690.18802773583850585No Hit
ATACAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2600.18173684504836995No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC2570.17963988145165802No Hit
ATACAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG2510.17544595425823406No Hit
ATACAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2500.1747469663926634No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2460.1719510149303808No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA2270.1586702454845384No Hit
ATACAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA2230.15587429402225578No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA2230.15587429402225578No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG2210.15447631829111447No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT2190.15307834255997316No Hit
ATACAGGGGGGGTGCTGGAGGAATGGCTTAGTGGTTAAGAGTACTAATTGTTCTTCCA2160.1509813789632612No Hit
ATACAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2160.1509813789632612No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA2030.1418945367108427No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCGA1900.13280769445842422No Hit
ATACAGGGGGGGCTGGAGAGATGGCTTGGCGGTTAAGAGCACTGGCTGCTCTTCCGAA1830.12791477939942963No Hit
ATACAGGGGGGCTCCTCAACCACACCACACCACATCCCCATTATACCCAAGACTTGCA1830.12791477939942963No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAAA1800.12581781580271767No Hit
ATACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.12511882793714701No Hit
ATACAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1760.12302186434043505No Hit
ATACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA1760.12302186434043505No Hit
ATACAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1730.12092490074372308No Hit
ATACAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1730.12092490074372308No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1710.11952692501258178No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCAACTGCTCTTCCG1660.11603198568472851No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA1650.11533299781915786No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1630.11393502208801654No Hit
ATACAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC1630.11393502208801654No Hit
ATACAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1620.11323603422244591No Hit
ATACAGGGGGATTGAATGTGGGCTTTATGCAGACCTCTAGGAGGGGCAGATAGATGGT1610.11253704635687525No Hit
ATACAGGGGGGGCTGTTGAGATGGCTCAGTGGGTAAGAGCACCCGAGTGCTCTTCCAA1590.11113907062573394No Hit
ATACAGGGGGGGGCTCGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1560.10904210702902196No Hit
ATACAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1560.10904210702902196No Hit
ATACAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAAG1550.10834311916345134No Hit
ATACAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCT1540.10764413129788067No Hit
ATACAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA1500.10484817983559805No Hit
ATACAGGGGAGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1490.1041491919700274No Hit
ATACAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1470.1027512162388861No Hit
ATACAGGGGGGGCTGGAGAGATAGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA1450.10135324050774479No Hit
ATACAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCAATGACTGCTCTTCTGA1440.10065425264217413No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC1440.10065425264217413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAC150.00715409352.09744326
AATCTCC150.00715409352.09744324
TAAATCA150.00715409352.09744326
CCAGCTC150.00715409352.09744326
ACGTCCC203.4058077E-452.09744326
TAAGTGG150.00715409352.09744326
CACCGTC150.00715409352.09744325
ACCGTCA150.00715409352.09744326
GTTTTAG251.6296819E-552.09744324
CCGTCAC150.00715409352.09744327
TCCAGCT150.00715409352.09744325
CCACCGT150.00715409352.09744324
GACGTCC203.4058077E-452.09744325
TCTTGCG150.00715409352.09744325
CCATATA203.4058077E-452.09744326
CTTGCGT150.00715409352.09744326
CTTAAAT150.00715409352.09744324
TTGGCCT203.4058077E-452.09744325
TTGCGTC150.00715409352.09744327
AAGTGGG150.00715409352.09744327
Produced by FastQC (version 0.11.5)