FastQCFastQC Report
Tue 31 May 2016
SRR212675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143133
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT42682.981842062976393No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC21401.4951129369188096No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT20691.4455087226565502No Hit
ATACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG20661.4434127699412433No Hit
ATACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC19521.3637665667595873No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17041.1905011422942298No Hit
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT14871.0388938958870422No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13770.9620422963257949No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA10670.7454605157440982No Hit
ATACAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT10160.7098293195838835No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA9810.6853765379053048No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8920.6231966073512049No Hit
ATACAGGGGGTTTTAATCATGGGCTTGGTATGTTAGTAACTGTTTTTATATTCACTTC7700.5379611969287306No Hit
ATACAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG7380.5156043679654587No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTTAGTGGTTAAGAGCACTGACTGCTCTTCCAA6860.4792745209001418No Hit
ATACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5730.40032696862358785No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC5710.3989296668133834No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA5180.36190116884296425No Hit
ATACAGGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4710.3290645763031586No Hit
ATACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT4610.32207806725213617No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.3060090964347844No Hit
ATACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC4330.30251584190927316No Hit
ATACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4120.287844172902126No Hit
ATACAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC3820.26688464574905857No Hit
ATACAGGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCTGA3680.2571035330776271No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3490.2438291658806844No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC3460.24173321316537769No Hit
ATACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3400.23754130773476415No Hit
ATACAGGGGGTCTCAGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCAAG3350.23404805320925293No Hit
ATACAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA3290.22985614777863944No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG3270.22845884596843494No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCAAA3270.22845884596843494No Hit
ATACAGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3270.22845884596843494No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG3260.2277601950633327No Hit
ATACAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCCAA3230.22566424234802596No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCCAA3190.22286963872761695No Hit
ATACAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC3010.21029392243577652No Hit
ATACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2890.20191011157454952No Hit
ATACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2860.1998141588592428No Hit
ATACAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2830.19771820614393606No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCGAA2540.17745732989597088No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCAGA2490.17396407537045963No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC2420.1690735190347439No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2360.16488161360413042No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA2280.15929240636331246No Hit
ATACAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2190.1530045482173922No Hit
ATACAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2130.14881264278677872No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA2120.14811399188167648No Hit
ATACAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2100.14671669007147198No Hit
ATACAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG2090.14601803916636974No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG2050.14322343554596076No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAAA2030.14182613373575625No Hit
ATACAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1950.13623692649493827No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCGA1940.13553827558983603No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCAACTGCTCTTCCG1920.13414097377963152No Hit
ATACAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCAATGACTGCTCTTCTGA1910.13344232287452928No Hit
ATACAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCA1810.12645581382350682No Hit
ATACAGGGGGGGCTGGAGAGATGGCTTGGCGGTTAAGAGCACTGGCTGCTCTTCCGAA1780.12435986110820006No Hit
ATACAGGGGGGGTGCTGGAGGAATGGCTTAGTGGTTAAGAGTACTAATTGTTCTTCCA1770.12366121020309782No Hit
ATACAGGGGGATTGAATGTGGGCTTTATGCAGACCTCTAGGAGGGGCAGATAGATGGT1700.11877065386738207No Hit
ATACAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1680.1173733520571776No Hit
ATACAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1680.1173733520571776No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA1660.11597605024697309No Hit
ATACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.1145787484367686No Hit
ATACAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAAG1610.11248279572146187No Hit
ATACAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1600.11178414481635962No Hit
ATACAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1570.10968819210105288No Hit
ATACAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1540.10759223938574612No Hit
ATACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAAACATCGAA1530.10689358848064388No Hit
ATACAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC1510.10549628667043938No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1500.10479763576533713No Hit
ATACAGGGGGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCCC1480.10340033395513264No Hit
ATACAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1480.10340033395513264No Hit
ATACAGGGGGGGCTGTTGAGATGGCTCAGTGGGTAAGAGCACCCGAGTGCTCTTCCAA1460.10200303214492813No Hit
ATACAGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1440.10060573033472366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTG550.052.3220569
AACGTAA150.007033344452.32205610
GGCAGTT251.5886699E-552.3220568
GGATGTA150.007033344452.3220568
GTCTCAG203.3341805E-452.32205610
GGATCTT203.3341805E-452.3220568
CATTATC150.007033344452.32205610
GCATTAT150.007033344452.3220569
GGAGTTT203.3341805E-452.3220568
GAAGTGT203.3341805E-452.3220569
GAACGTA150.007033344452.3220569
GGTGCCT401.7753337E-952.3220568
GTTTTAA1200.052.32205610
GGCATTA203.3341805E-452.3220568
GGCATAG150.007033344452.3220569
GGCATAC203.3341805E-452.3220568
GGGATGC503.6379788E-1252.3220567
GCTCATT150.007033344452.32205610
AGACTTG251.6424638E-552.02873250
TATCACT150.00719152952.02873250
Produced by FastQC (version 0.11.5)