FastQCFastQC Report
Tue 31 May 2016
SRR212674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132540
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC34772.623358985966501No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC22471.6953372566772293No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC21231.6017805945374983No Hit
ATACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA18881.424475629998491No Hit
ATACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA17671.3331824354911723No Hit
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT16991.281877169156481No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16421.2388712841406369No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16161.2192545646597255No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT15621.1785121472762938No Hit
ATACAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC13641.0291232835370454No Hit
ATACAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA9190.6933755847291384No Hit
ATACAGGGGGTTTTAATCATGGGCTTGGTATGTTAGTAACTGTTTTTATATTCACT7520.5673758865248227No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTTAGTGGTTAAGAGCACTGACTGCTCTTCC6390.48211860570393844No Hit
ATACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT6250.47155575675267847No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCA5990.451939037271767No Hit
ATACAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5980.4511845480609627No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC5840.4406216991097027No Hit
ATACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5650.4262864041044213No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG5220.39384336803983705No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.3606458427644485No Hit
ATACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4490.33876565565112415No Hit
ATACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3810.28746038931643275No Hit
ATACAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3750.282933454051607No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3600.2716161158895428No Hit
ATACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3600.2716161158895428No Hit
ATACAGGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCT3590.2708616266787385No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3520.2655802022031085No Hit
ATACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3460.2610532669382828No Hit
ATACAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCC3460.2610532669382828No Hit
ATACAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3170.23917307982495847No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3110.23464614456013277No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCC2960.2233288063980685No Hit
ATACAGGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2950.2225743171872642No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC2860.21578391429002566No Hit
ATACAGGGGGTCTCAGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCA2700.2037120869171571No Hit
ATACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2690.20295759770635277No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC2650.19993964086313568No Hit
ATACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2560.19314923796589709No Hit
ATACAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2490.18786781349026707No Hit
ATACAGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2370.17881394296061567No Hit
ATACAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2270.1712690508525728No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCG2250.16976007243096422No Hit
ATACAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2220.16749660479855139No Hit
ATACAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2200.1659876263769428No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC2180.16447864795533423No Hit
ATACAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT2150.1622151803229214No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC2090.15768824505809567No Hit
ATACAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA2030.15316130979326995No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCC2000.1508978421608571No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1990.15014335295005282No Hit
ATACAGGGGGCCCTTTATGCCAGGAAATTCCGGCTTTCTTGCTCGGAGATGAACGG1900.14335295005281423No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC1890.14259846084200997No Hit
ATACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.1403349932095971No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTC1840.13882601478798853No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1820.13731703636637996No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1820.13731703636637996No Hit
ATACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1760.13279010110155426No Hit
ATACAGGGGGGGTGCTGGAGGAATGGCTTAGTGGTTAAGAGTACTAATTGTTCTTC1740.13128112267994568No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCAACTGCTCTTC1700.12826316583672853No Hit
ATACAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCAATGACTGCTCTTCT1680.12675418741511996No Hit
ATACAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1660.12524520899351138No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA1660.12524520899351138No Hit
ATACAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1650.12449071978270711No Hit
ATACAGGGGGATTGAATGTGGGCTTTATGCAGACCTCTAGGAGGGGCAGATAGATG1640.12373623057190283No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTC1570.11845480609627282No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1560.11770031688546854No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1530.11543684925305567No Hit
ATACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.1146823600422514No Hit
ATACAGGGGGGGCTGTTGAGATGGCTCAGTGGGTAAGAGCACCCGAGTGCTCTTCC1520.1146823600422514No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCT1450.1094009355666214No Hit
ATACAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGA1420.10713746793420853No Hit
ATACAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1400.10562848951259998No Hit
ATACAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1380.1041195110909914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCAAA204.067886E-450.2525749
GCAAGCC204.067886E-450.2525749
GTCGTGG204.067886E-450.2525750
CTAATCG252.0163081E-550.2525749
TAATCGC252.0163081E-550.2525750
CAAGCCC204.067886E-450.2525750
AGATAGA204.067886E-450.2525748
TAGGTTT204.067886E-450.2525749
ATCCCCT451.2732926E-1050.25256748
CAAGACT150.00824859150.25256748
GCAAGCA600.050.25256750
ACCATGA150.00824859150.25256749
TGATTTG150.00824859150.25256750
ATCAGAC150.00824859150.25256748
TCTGTCC150.00824859150.25256750
GTCCCGA150.00824859150.25256749
AATGGCC301.0052045E-650.25256749
TATCACT150.00824859150.25256750
AGACTTG150.00824859150.25256750
GCCAAAT150.00824859150.25256749
Produced by FastQC (version 0.11.5)