FastQCFastQC Report
Tue 31 May 2016
SRR212673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124284
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC35592.863602716359306No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC21231.7081844807054811No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC20961.6864600431270316No Hit
ATACAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC19991.6084129896044543No Hit
ATACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA18671.502204628109813No Hit
ATACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA17411.4008239194103826No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC17191.3831225258279425No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC16741.3469151298638602No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT14981.2053039812043385No Hit
ATACAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14231.1449583212642012No Hit
ATACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT9470.7619645328441312No Hit
ATACAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA8510.6847220881207556No Hit
ATACAGGGGGTTTTAATCATGGGCTTGGTATGTTAGTAACTGTTTTTATATTCACT6710.5398925042644266No Hit
ATACAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG6150.4948344115091242No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTTAGTGGTTAAGAGCACTGACTGCTCTTCC5780.46506388593865666No Hit
ATACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC5350.430465707572978No Hit
ATACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5250.42241961958095975No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCA5100.4103504875929323No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG4710.37897074442406103No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.37494770042805187No Hit
ATACAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4630.3725338740304464No Hit
ATACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4560.3669016124360336No Hit
ATACAGGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3720.29931447330308003No Hit
ATACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3590.2888545589134563No Hit
ATACAGGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCT3430.27598081812622705No Hit
ATACAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3320.2671301213350069No Hit
ATACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA3290.26471629493740145No Hit
ATACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3260.26230246853979594No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3160.25425638054777766No Hit
ATACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3160.25425638054777766No Hit
ATACAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3140.252647162949374No Hit
ATACAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCC3020.24299185735895207No Hit
ATACAGGGGCTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2950.23735959576453927No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC2760.22207202857970457No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCC2730.21965820218209905No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC2690.21643976698529177No Hit
ATACAGGGGGTCTCAGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCA2650.21322133178848443No Hit
ATACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2530.2035660261980625No Hit
ATACAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2500.201152199800457No Hit
ATACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2490.2003475910012552No Hit
ATACAGGGGTGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2470.19873837340285153No Hit
ATACAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCCG2250.1810369798204113No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC2200.17701393582440217No Hit
ATACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC2160.17379550062759488No Hit
ATACAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2100.1689678478323839No Hit
ATACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT2090.16816323903318206No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC2060.16574941263557658No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2040.16414019503717292No Hit
ATACAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC2000.1609217598403656No Hit
ATACAGGGGGCCCTTTATGCCAGGAAATTCCGGCTTTCTTGCTCGGAGATGAACGG1960.1577033246435583No Hit
ATACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.15368028064754916No Hit
ATACAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1810.14563419265553088No Hit
ATACAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1810.14563419265553088No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTC1770.14241575745872356No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCTTCC1730.13919732226191625No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC1700.13678349586431077No Hit
ATACAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCAATGACTGCTCTTCT1700.13678349586431077No Hit
ATACAGGGGGATTGAATGTGGGCTTTATGCAGACCTCTAGGAGGGGCAGATAGATG1680.1351742782659071No Hit
ATACAGGGGGGGTGCTGGAGGAATGGCTTAGTGGTTAAGAGTACTAATTGTTCTTC1670.13436966946670528No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCAACTGCTCTTC1670.13436966946670528No Hit
ATACAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1590.12793279907309066No Hit
ATACAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1580.12712819027388883No Hit
ATACAGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1580.12712819027388883No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA1480.11908210228187056No Hit
ATACAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1420.1142544494866596No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1410.11344984068745775No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1400.11264523188825593No Hit
ATACAGGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC1350.10862218789224679No Hit
ATACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC1300.10459914389623765No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTC1300.10459914389623765No Hit
ATACAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1300.10459914389623765No Hit
ATACAGGGGGGGGCTCGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1290.10379453509703582No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGTGCCGACTGCTCTTCCA1290.10379453509703582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTG204.0883492E-450.19902850
TGCAATG252.0283267E-550.19902450
GCGCGAT150.00828221450.19902450
TAATCGC150.00828221450.19902450
TTGTGCT150.00828221450.19902450
AACATCC150.00828221450.19902450
CATCGAA252.0283267E-550.19902450
CGCGACC204.0965388E-450.17868842
CAAGACT204.0965388E-450.17868848
CCTATCG402.566594E-950.17868845
CGGAGAT355.0928065E-850.17868844
TATACCC204.0965388E-450.17868842
TTGTATC204.0965388E-450.17868843
CGCCCAC204.0965388E-450.17868846
ACGCACC204.0965388E-450.17868849
GTCAAGA204.0965388E-450.17868846
GTCCGCG204.0965388E-450.17868849
TATCGGG402.566594E-950.17868847
GTATCGC204.0965388E-450.17868845
CCCACAG204.0965388E-450.17868848
Produced by FastQC (version 0.11.5)