FastQCFastQC Report
Tue 31 May 2016
SRR212672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114505
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC13881.212174140867211No Hit
TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC10160.8872974979258548No Hit
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9140.7982184184096763No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8750.764158770359373No Hit
TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8310.7257325007641588No Hit
TGAAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7890.6890528797869089No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7250.6331601240120519No Hit
TGAAGCGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT6980.609580367669534No Hit
TGAAGCGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGCTCTTCCA6980.609580367669534No Hit
TGAAGCGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC6760.590367232871927No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6630.5790140168551592No Hit
TGAAGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4930.43054888432819527No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4860.4244356141653203No Hit
TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4640.4052224793677132No Hit
TGAAGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4360.3807693987162133No Hit
TGAAGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT4210.3676695340814812No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3980.34758307497489194No Hit
TGAAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3840.33535653464914195No Hit
TGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3150.2750971573293743No Hit
TGAAGCGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2960.2585039954587136No Hit
TGAAGCGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG2940.2567573468407493No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2520.2200777258634994No Hit
TGAAGCGGGAGGGGCTGGAGAGATGGCTCAGCAGGTTAAGAGCCCTGACTGCTCTTCC2500.21833107724553513No Hit
TGAAGCGGGGGGCCATACTGTTCTGTCTGAGGGAAGTAGGGAAAAGATTGCGGATCTC2500.21833107724553513No Hit
TGAAGCGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2480.21658442862757085No Hit
TGAAGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2280.19911794244792802No Hit
TGAAGCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2260.19737129382996374No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC2180.19038469935810665No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC2050.1790314833413388No Hit
TGAAGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2030.17728483472337453No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTTCTCC2020.17641151041439238No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.17553818610541025No Hit
TGAAGCGGGAACTTCCTAAGATTTAGCTAATACACGAAGACAAATCCTAGGGAAGTGT1960.17117156456049953No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1920.16767826732457097No Hit
TGAAGCGGGCTGTCATAGGCTTTGGACTCAGCTTTTGAGACGGAAGCGTTAGGTTTTG1860.16243832147067813No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1840.16069167285271385No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA1830.1598183485437317No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1780.155451726998821No Hit
TGAAGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1760.15370507838085673No Hit
TGAAGCGGGGGGCTGGAGAGATGACTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAA1750.1528317540718746No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1720.15021178114492817No Hit
TGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1690.14759180821798176No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1620.14147853805510677No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCGA1620.14147853805510677No Hit
TGAAGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1610.1406052137461246No Hit
TGAAGCGGGGATTATAGTGGCTTTTATACTTAAATTTCTCTTCTTCCAGGAGCCCCAA1570.13711191651019605No Hit
TGAAGCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1520.13274529496528537No Hit
TGAAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1520.13274529496528537No Hit
TGAAGCGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1500.13099864634732108No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.12663202480241037No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1430.12488537618444609No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1410.12313872756648181No Hit
TGAAGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1410.12313872756648181No Hit
TGAAGCGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTT1390.12139207894851754No Hit
TGAAGCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1380.1205187546395354No Hit
TGAAGCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1360.11877210602157112No Hit
TGAAGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1280.11178551154971399No Hit
TGAAGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.10916553862276757No Hit
TGAAGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1250.10916553862276757No Hit
TGAAGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1250.10916553862276757No Hit
TGAAGCGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1220.10654556569582115No Hit
TGAAGCGGGGCTTTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC1220.10654556569582115No Hit
TGAAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1210.105672241386839No Hit
TGAAGCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1200.10479891707785687No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG1190.10392559276887472No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.10392559276887472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAG203.426007E-452.02622644
AGCCGTC203.426007E-452.02622639
AATCCTA203.426007E-452.02622643
AAATCCT203.426007E-452.02622642
GCGACAT203.426007E-452.02622637
ATGGCTA203.426007E-452.02622642
ACTTGGA203.426007E-452.02622639
ACGCACC203.426007E-452.02622650
TCACAAT203.426007E-452.02622641
GACAAAT203.426007E-452.02622639
CAATTAT203.426007E-452.02622644
CGACATG203.426007E-452.02622638
TGAGTGG203.426007E-452.02622650
TCCTAGG203.426007E-452.02622645
TATCTCA203.426007E-452.02622648
ACCAGAC203.426007E-452.02622648
AGACCAG203.426007E-452.02622651
CTCATCA203.426007E-452.02622651
ATGACTT203.426007E-452.02622636
AATTATC203.426007E-452.02622645
Produced by FastQC (version 0.11.5)