FastQCFastQC Report
Tue 31 May 2016
SRR212671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122012
Sequences flagged as poor quality0
Sequence length58
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC14261.1687375012293872No Hit
TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC10630.8712257810707144No Hit
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10540.8638494574304166No Hit
TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8830.7236993082647608No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8540.6999311543126906No Hit
TGAAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC8270.6778021833917975No Hit
TGAAGCGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC7370.6040389469888208No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7360.6032193554732321No Hit
TGAAGCGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGCTCTTCCA7190.5892862997082254No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6780.5556830475690916No Hit
TGAAGCGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT6660.5458479493820281No Hit
TGAAGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5020.4114349408254926No Hit
TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4960.4065173917319608No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4720.3868471953578337No Hit
TGAAGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT4560.37373373110841557No Hit
TGAAGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4290.35160476018752257No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3990.3270170147198636No Hit
TGAAGCGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3590.2942333540963184No Hit
TGAAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3520.288496213487198No Hit
TGAAGCGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG3410.27948070681572307No Hit
TGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3250.26636724256630495No Hit
TGAAGCGGGGGGCCATACTGTTCTGTCTGAGGGAAGTAGGGAAAAGATTGCGGATCTC2870.23522276497393702No Hit
TGAAGCGGGAGGGGCTGGAGAGATGGCTCAGCAGGTTAAGAGCCCTGACTGCTCTTCC2670.2188309346621644No Hit
TGAAGCGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2670.2188309346621644No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2600.213093794053044No Hit
TGAAGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2450.20079992131921448No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTTCTCC2350.1926040061633282No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC2240.18358849949185327No Hit
TGAAGCGGGCTGTCATAGGCTTTGGACTCAGCTTTTGAGACGGAAGCGTTAGGTTTTG2210.18112972494508736No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2210.18112972494508736No Hit
TGAAGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2170.17785135888273285No Hit
TGAAGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2160.1770317673671442No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA2130.17457299282037833No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.16965544372684654No Hit
TGAAGCGGGAACTTCCTAAGATTTAGCTAATACACGAAGACAAATCCTAGGGAAGTGT2050.16801626069566927No Hit
TGAAGCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2010.16473789463331476No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1980.16227912008654888No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1940.15900075402419434No Hit
TGAAGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1890.15490279644625118No Hit
TGAAGCGGGGGGCTGGAGAGATGACTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAA1870.15326361341507394No Hit
TGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1850.15162443038389667No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1820.1491656558371308No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1790.14670688129036488No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1670.1368717831033013No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1610.13195423400976955No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1590.13031505097859228No Hit
TGAAGCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1540.12621709340064913No Hit
TGAAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1490.12211913582270598No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCGA1460.11966036127594008No Hit
TGAAGCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1460.11966036127594008No Hit
TGAAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1460.11966036127594008No Hit
TGAAGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.11802117824476283No Hit
TGAAGCGGGGATTATAGTGGCTTTTATACTTAAATTTCTCTTCTTCCAGGAGCCCCAA1430.11720158672917419No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.11556240369799693No Hit
TGAAGCGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1410.11556240369799693No Hit
TGAAGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1380.11310362915123104No Hit
TGAAGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1370.1122840376356424No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.10900567157328789No Hit
TGAAGCGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA1330.10900567157328789No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG1320.10818608005769925No Hit
TGAAGCGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTT1230.10080975641740156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTG203.4092925E-452.08049852
CTCATCA203.4092925E-452.08049851
TTTACGA251.6313968E-552.0804952
AGCCCCA150.00716079552.0804951
TATGGGT150.00716079552.0804952
CGACTGA150.00716079552.0804951
TGTCCCC150.00716079552.0804952
CATCAAC203.4231486E-452.03775426
TTAGGTT307.8961966E-752.0377549
GAGCTAG150.007184093452.0377527
TGAACCC150.007184093452.0377549
ACGTGTA150.007184093452.0377527
CACAGTG150.007184093452.0377526
GTCCGAC150.007184093452.0377526
CGAGCTA150.007184093452.0377526
CACGTGT150.007184093452.0377526
AAAATGC150.007184093452.0377549
AAATGCC150.007184093452.0377550
AGCGTTA307.9186066E-752.01640745
ATCCTAG203.4300933E-452.01640744
Produced by FastQC (version 0.11.5)