FastQCFastQC Report
Tue 31 May 2016
SRR212670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127561
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC15531.2174567461841785No Hit
TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC9570.750229302059407No Hit
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9060.710248430162824No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8690.6812426995711856No Hit
TGAAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC8660.6788908835772689No Hit
TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8370.6561566623027414No Hit
TGAAGCGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT7870.6169597290707974No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7700.6036327717719366No Hit
TGAAGCGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC7680.6020648944426588No Hit
TGAAGCGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGCTCTTCCA6840.536214046612993No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6140.48133834008827153No Hit
TGAAGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5240.4107838602707724No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5000.3919693323194393No Hit
TGAAGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4570.35825996973996754No Hit
TGAAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG4560.35747603107532866No Hit
TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT4090.32063091383730136No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3860.30260032455060715No Hit
TGAAGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT3810.2986806312274128No Hit
TGAAGCGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3510.27516247128824645No Hit
TGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3170.24850855669052452No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2970.23282978339774696No Hit
TGAAGCGGGGGGCCATACTGTTCTGTCTGAGGGAAGTAGGGAAAAGATTGCGGATCTC2710.2124473781171361No Hit
TGAAGCGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2680.21009556212321948No Hit
TGAAGCGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG2490.19520072749508077No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTTCTCC2480.19441678883044192No Hit
TGAAGCGGGAGGGGCTGGAGAGATGGCTCAGCAGGTTAAGAGCCCTGACTGCTCTTCC2460.19284891150116415No Hit
TGAAGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2330.18265770886085872No Hit
TGAAGCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2210.17325044488519217No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGA2140.16776287423272004No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC2120.16619499690344228No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.1654110582388034No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2090.16384318090952563No Hit
TGAAGCGGGAACTTCCTAAGATTTAGCTAATACACGAAGACAAATCCTAGGGAAGTGT2040.15992348758633124No Hit
TGAAGCGGGCTGTCATAGGCTTTGGACTCAGCTTTTGAGACGGAAGCGTTAGGTTTTG2020.15835561025705347No Hit
TGAAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1940.15208410093994246No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1890.14816440761674807No Hit
TGAAGCGGGGGGCTGGAGAGATGACTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAA1870.1465965302874703No Hit
TGAAGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1800.14110895963499817No Hit
TGAAGCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1770.13875714364108152No Hit
TGAAGCGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1770.13875714364108152No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1730.13562138898252601No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1730.13562138898252601No Hit
TGAAGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1730.13562138898252601No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1640.1285659410007761No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1630.1277820023361372No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.12543018634222058No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1580.12386230901294283No Hit
TGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1550.1215104930190262No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1540.12072655435438732No Hit
TGAAGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1500.11759079969583179No Hit
TGAAGCGGGGCTTTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC1500.11759079969583179No Hit
TGAAGCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1410.1105353517140819No Hit
TGAAGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1410.1105353517140819No Hit
TGAAGCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1400.10975141304944301No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.10818353572016526No Hit
TGAAGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1380.10818353572016526No Hit
TGAAGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1360.1066156583908875No Hit
TGAAGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1350.10583171972624861No Hit
TGAAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1340.10504778106160974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC700.052.325166
GGCAAGC203.3318085E-452.325169
GATCACA203.3318085E-452.325169
CGGGATA700.052.325166
AAGCTGT203.3318085E-452.3251610
CGGGTAT251.5871157E-552.3251576
GGTACCT458.54925E-1152.3251578
GACACTC150.007029928352.3251579
AACCACA251.5871157E-552.32515710
AAGCATG1500.052.32515710
GCGGGTC1950.052.3251575
GCGGGAT4900.052.3251575
GGAACCT150.007029928352.3251578
GGAACCA307.6040305E-752.3251578
CGGGATT1050.052.3251576
GGAAAGT150.007029928352.3251578
GAACCAC251.5871157E-552.3251579
GGATTCA150.007029928352.3251578
AAGCTTA150.007029928352.32515710
GACTAGT150.007029928352.32515710
Produced by FastQC (version 0.11.5)