FastQCFastQC Report
Tue 31 May 2016
SRR212669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185863
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC22011.1842055707698682No Hit
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT21191.1400870533672651No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC20621.1094193034654558No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC18010.9689932907571706No Hit
TGAAGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC14100.7586232870447587No Hit
TGAAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC12150.6537073005385687No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11440.615507120836315No Hit
TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA11130.5988281691353309No Hit
TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA10360.5573998052328866No Hit
TGAAGCGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT7710.41482166972447454No Hit
TGAAGCGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC7320.39383847242323644No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG6980.3755454286221572No Hit
TGAAGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6830.367474968121681No Hit
TGAAGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA6750.36317072252142707No Hit
TGAAGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6280.33788327961993514No Hit
TGAAGCGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5460.29376476221733216No Hit
TGAAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA5440.29268870081726867No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC5300.2851562710168242No Hit
TGAAGCGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGCTCTTC5250.2824661175166655No Hit
TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA5030.2706294421159672No Hit
TGAAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA4510.24265184571431644No Hit
TGAAGCGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT4270.2297391089135546No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTTCT4260.22920107821352287No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4180.2248968326132689No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.218440464212888No Hit
TGAAGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.20983197301238007No Hit
TGAAGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3720.20014742041180872No Hit
TGAAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3720.20014742041180872No Hit
TGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3560.1915389292113008No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3520.1893868064111738No Hit
TGAAGCGGGGGGCCATACTGTTCTGTCTGAGGGAAGTAGGGAAAAGATTGCGGATC3420.1840064994108564No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC3260.17539800821034848No Hit
TGAAGCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3130.16840360910993582No Hit
TGAAGCGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3120.16786557840990407No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC3000.16140921000952316No Hit
TGAAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2920.1571049644092692No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.1560289030092057No Hit
TGAAGCGGGCTGTCATAGGCTTTGGACTCAGCTTTTGAGACGGAAGCGTTAGGTTT2850.153338749509047No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCT2820.15172465740895175No Hit
TGAAGCGGGAGGGGCTGGAGAGATGGCTCAGCAGGTTAAGAGCCCTGACTGCTCTT2760.14849647320876128No Hit
TGAAGCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC2730.14688238110866608No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2700.14526828900857083No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCC2580.13881192060818992No Hit
TGAAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2570.13827388990815817No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.13612176710803117No Hit
TGAAGCGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGGTCTTC2410.12966539870765026No Hit
TGAAGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2380.12805130660755504No Hit
TGAAGCGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2370.1275132759075233No Hit
TGAAGCGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2360.12697524520749154No Hit
TGAAGCGGGGCTTTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG2280.1226709996072376No Hit
TGAAGCGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2080.11191038560660271No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC2070.11137235490657096No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2060.11083432420653921No Hit
TGAAGCGGGGATTATAGTGGCTTTTATACTTAAATTTCTCTTCTTCCAGGAGCCCC2020.10868220140641226No Hit
TGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1990.10706810930631702No Hit
TGAAGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1980.10653007860628527No Hit
TGAAGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1980.10653007860628527No Hit
TGAAGCGGGAACTTCCTAAGATTTAGCTAATACACGAAGACAAATCCTAGGGAAGT1960.10545401720622179No Hit
TGAAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1950.10491598650619004No Hit
TGAAGCGGGGGGCTGGAGAGATGACTCAGTGGTTAAGAGCACCGACTGCTCTTCCG1880.10114977160596784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC204.0363925E-450.3424948
GTTAGGT354.9816663E-850.3424948
CCTCCGT204.0363925E-450.3424948
ATATACC150.0081956750.3424949
TCCGTCG204.0363925E-450.3424950
CATCGAA507.2759576E-1250.3424950
CGGTATG150.0081956750.3424950
TACAGAA251.9982366E-550.3424950
TCGGTAT150.0081956750.3424948
TTAGGGC252.0079087E-550.3014447
AACGTGC150.00822214950.30143746
CTAAGTC150.00822214950.30143746
TAAGTCA150.00822214950.30143747
CGTTAGG355.0138624E-850.30143747
GTGCTCC204.0526805E-450.30143746
ACGTGCC150.00822214950.30143747
GTGTTTT150.00823098950.28777344
CGACGGC150.00823098950.28777344
GACGGCT150.00823098950.28777345
CCGACGG150.00823098950.28777343
Produced by FastQC (version 0.11.5)