FastQCFastQC Report
Tue 31 May 2016
SRR212668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173392
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC22061.27226169604134No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC18771.0825182246009044No Hit
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18411.061756021039033No Hit
TGAAGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC17581.013887607271385No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC16550.9544846359693642No Hit
TGAAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC11880.6851527175417551No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC11020.6355541201439513No Hit
TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA9890.5703838700747439No Hit
TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9570.5519285780197472No Hit
TGAAGCGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8220.47407031466272953No Hit
TGAAGCGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC7670.4423502814432039No Hit
TGAAGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT7450.42966226815539355No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG7100.4094767924702408No Hit
TGAAGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA6940.4002491464427425No Hit
TGAAGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT6480.37371966411368457No Hit
TGAAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA5930.3419996308941589No Hit
TGAAGCGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5560.3206606994555689No Hit
TGAAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA5430.31316323705822646No Hit
TGAAGCGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGCTCTTC5300.305665774660884No Hit
TGAAGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC4660.2687551905508905No Hit
TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA4500.25952754452339205No Hit
TGAAGCGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT4280.24683953123558178No Hit
TGAAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4150.23934206883823936No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTTCT4050.23357479007105286No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.22319368829011718No Hit
TGAAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3660.21108240287902558No Hit
TGAAGCGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT3640.20992894712558827No Hit
TGAAGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.20416166835840174No Hit
TGAAGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3480.20070130109808987No Hit
TGAAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3400.19608747808434068No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3220.185706376303405No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC3210.18512964842668636No Hit
TGAAGCGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3150.18166928116637446No Hit
TGAAGCGGGGGGGGCTGGAGAGATGGCTCAGTAGTTAAGAGCACTGGCTGGTCTTC2920.16840454000184554No Hit
TGAAGCGGGGGGCCATACTGTTCTGTCTGAGGGAAGTAGGGAAAAGATTGCGGATC2920.16840454000184554No Hit
TGAAGCGGGGCTTTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG2890.1666743563716896No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC2840.16379071698809633No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.16148380548122174No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2770.1597536218510658No Hit
TGAAGCGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2700.15571652671403524No Hit
TGAAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2680.15456307096059796No Hit
TGAAGCGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2620.15110270370028606No Hit
TGAAGCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC2570.14821906431669282No Hit
TGAAGCGGGAGGGGCTGGAGAGATGGCTCAGCAGGTTAAGAGCCCTGACTGCTCTT2550.1470656085632555No Hit
TGAAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2430.14014487404263173No Hit
TGAAGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2340.13495432315216388No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCC2320.1338008673987266No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.13207068376857065No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCT2250.12976377226169603No Hit
TGAAGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC2160.12457322137122821No Hit
TGAAGCGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2080.11995939835747901No Hit
TGAAGCGGGCTGTCATAGGCTTTGGACTCAGCTTTTGAGACGGAAGCGTTAGGTTT2000.1153455753437298No Hit
TGAAGCGGGAACTTCCTAAGATTTAGCTAATACACGAAGACAAATCCTAGGGAAGT2000.1153455753437298No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC1980.11419211959029252No Hit
TGAAGCGGGCTCTGCGTTTCCGCGTTTCCACTTTTCCGCGCAGAAGCCGCAAACAT1960.11303866383685522No Hit
TGAAGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1930.11130848020669927No Hit
TGAAGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1780.10265756205591954No Hit
TGAAGCGGGGGGCTGGAGAGATGACTCAGTGGTTAAGAGCACCGACTGCTCTTCCG1740.10035065054904493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA650.050.23249450
CAAACAT355.0657036E-850.2324950
TACAGAA204.0794577E-450.2324950
ACAAGCG301.0098174E-650.2324950
GTTTTAC204.0853137E-450.217949
AGATTAA150.00827544550.21789649
GGTCGAG204.0970457E-450.18873648
CCTAGGG252.0341804E-550.18873246
ACACACG150.00829447450.18873246
CCAGCCT252.0341804E-550.18873248
CGTTAGG252.0341804E-550.18873247
ACTACAC150.00829447450.18873246
TTGCGGA301.0159092E-650.18873248
AACTGCG150.00829447450.18873246
CACACGA150.00829447450.18873247
AAAATTC252.0341804E-550.18873247
CTAGGGA252.0341804E-550.18873247
AGCGTTA252.0376789E-550.17416445
ATCCTAG252.0376789E-550.17416444
AATCCTA252.0376789E-550.17416443
Produced by FastQC (version 0.11.5)