FastQCFastQC Report
Tue 31 May 2016
SRR212667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43572
Sequences flagged as poor quality0
Sequence length58
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT20074.606169099421646No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC17704.06224180666483No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7741.776370145965299No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6541.5009639217846322No Hit
CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5951.3655558615624712No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG5521.2668686312310657No Hit
CCGCTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4731.0855595336454604No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4591.0534288074910494No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3750.8606444505645827No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT3470.7963829982557605No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT3340.7665473239695216No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2760.6334343156155329No Hit
CCGCTAGGGCCTTTTCCTGTGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2320.5324520334159552No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA2120.4865509960525108No Hit
CCGCTAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA2110.48425594418433854No Hit
CCGCTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2060.47278068484347746No Hit
CCGCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1830.41999449187551635No Hit
CCGCTAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1630.374093454512072No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1600.3672082989075553No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1510.34655283209400534No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1510.34655283209400534No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1490.3419627283576609No Hit
CCGCTAGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG1460.3350775727531442No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1460.3350775727531442No Hit
CCGCTAGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTCCAA1450.332782520884972No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA1410.3236023134122831No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1370.3144221059395942No Hit
CCGCTAGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTT1360.312127054071422No Hit
CCGCTAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA1340.30753695033507755No Hit
CCGCTAGGGGCCACAGCCATCTCCAGGACTGAATTATGTTCCAAAAAAAAGCATCCCC1260.28917653538969984No Hit
CCGCTAGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCGCC1210.2777012760488387No Hit
CCGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1200.27540622418066646No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA1180.270816120444322No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGAAC1170.2685210685761498No Hit
CCGCTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1040.23868539428991095No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1040.23868539428991095No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1020.2340952905535665No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1000.22950518681722204No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA950.21802992747636096No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA940.21573487560818874No Hit
CCGCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT910.20884972000367205No Hit
CCGCTAGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGACA910.20884972000367205No Hit
CCGCTAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC880.20196456439915542No Hit
CCGCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA870.19966951253098322No Hit
CCGCTAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG860.19737446066281097No Hit
CCGCTAGGGGCTCCGCCGTCTGCCACTGCCGCCTGGGCTCACTGAGTGGTTCATCTGG850.19507940879463875No Hit
CCGCTAGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCCCCCCATATCGG850.19507940879463875No Hit
CCGCTAGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA840.19278435692646653No Hit
CCGCTAGGGGGGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTTTGCCCGCCGTGAT810.18589920132194987No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCC780.17901404571743323No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA780.17901404571743323No Hit
CCGCTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT780.17901404571743323No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCC740.16983383824474432No Hit
CCGCTAGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT720.16524373450839988No Hit
CCGCTAGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCCCCCCCTATCGG720.16524373450839988No Hit
CCGCTAGGGTAGAAGGGGGTTTTCTCAACCTCTGGCTGCTGAGAGCGATACTTCAGAA700.16065363077205547No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA690.15835857890388322No Hit
CCGCTAGGGGAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGATTGCTCTTCCAG690.15835857890388322No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA680.156063527035711No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT680.156063527035711No Hit
CCGCTAGGGGAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTG670.15376847516753878No Hit
CCGCTAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA660.15147342329936656No Hit
CCGCTAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA650.14917837143119433No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.14458826769484992No Hit
CCGCTAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGC610.13999816395850545No Hit
CCGCTAGGGGAGCTGGCTGGTCGGGGACAGGAAAGAAGGAAGAACTAACTCAGATAAA610.13999816395850545No Hit
CCGCTAGGGGCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCACG610.13999816395850545No Hit
CCGCTAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA600.13770311209033323No Hit
CCGCTAGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTT600.13770311209033323No Hit
CCGCTAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG600.13770311209033323No Hit
CCGCTAGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCCCCCCCTATCGG600.13770311209033323No Hit
CCGCTAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC600.13770311209033323No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTCTTCTGA590.135408060222161No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC580.1331130083539888No Hit
CCGCTAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA570.13081795648581657No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC550.12622785274947215No Hit
CCGCTAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCA540.12393280088129992No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGCCC540.12393280088129992No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCGA540.12393280088129992No Hit
CCGCTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT530.1216377490131277No Hit
CCGCTAGGGGACTATGCGTGGGCTGGAGCAACCGCCTGCTGGGTGCAAACCCTTTGCG530.1216377490131277No Hit
CCGCTAGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT520.11934269714495548No Hit
CCGCTAGGGGCCTTTCCTCTGGCGGCAGCCATCAGGTGAGCCAAGATGGGTGCATACA510.11704764527678325No Hit
CCGCTAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC510.11704764527678325No Hit
CCGCTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA510.11704764527678325No Hit
CCGCTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG500.11475259340861102No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT500.11475259340861102No Hit
CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA500.11475259340861102No Hit
CCGCTAGGGGCCCTTGGGTCCAGGAAATGGTTGTTGTGACGAGAATTCCCATCTCTTG490.11245754154043883No Hit
CCGCTAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC490.11245754154043883No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC490.11245754154043883No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGGGCGGGGCCTTCTCCT490.11245754154043883No Hit
CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC480.1101624896722666No Hit
CCGCTAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA470.10786743780409437No Hit
CCGCTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG470.10786743780409437No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTAAGTAAGAGCACCCGACTGCTCTTCC460.10557238593592215No Hit
CCGCTAGGGGAGTAATGGGCTCCACCCTTCCTTGCTCCCAGGGTATCAGAAGAACAGA460.10557238593592215No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC460.10557238593592215No Hit
CCGCTAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG450.10327733406774993No Hit
CCGCTAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT450.10327733406774993No Hit
CCGCTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA450.10327733406774993No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGTTCTTCCGA440.10098228219957771No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA440.10098228219957771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT251.6172895E-552.0621438
TGCATTG150.007139919352.0621436
CGTCACG150.007139919352.0621437
GGATGGG150.007139919352.0621442
TTGTTCC150.007139919352.0621440
TCCCATC150.007139919352.0621447
CATTGTT150.007139919352.0621438
CCTATCG203.3907854E-452.0621451
GTCATCA251.6172895E-552.0621446
ACTGATT150.007139919352.0621442
TCCTATT251.6172895E-552.0621439
ACGTCTC150.007139919352.0621441
GAGTGGT150.007139919352.0621444
GATACAA503.6379788E-1252.0621451
ACCATGA150.007139919352.0621449
GCAGGTT150.007139919352.0621444
CACGTCT150.007139919352.0621440
GTTCTGG150.007139919352.0621448
GGTCTGC150.007139919352.0621443
TGATTGC150.007139919352.0621444
Produced by FastQC (version 0.11.5)