FastQCFastQC Report
Tue 31 May 2016
SRR212666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45185
Sequences flagged as poor quality0
Sequence length58
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT21024.65198627863229No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC18013.985836007524621No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7971.7638596879495407No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6931.533694810224632No Hit
CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6241.3809892663494523No Hit
CCGCTAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG6011.3300874183910592No Hit
CCGCTAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4370.9671351112094723No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4240.9383645014938586No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3570.7900852052672347No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT3460.765740843200177No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT3420.7568883479030653No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2560.5665596990151599No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA2460.5444284607723803No Hit
CCGCTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2220.49131348898970895No Hit
CCGCTAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA2210.489100365165431No Hit
CCGCTAGGGCCTTTTCCTGTGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2180.48246099369259715No Hit
CCGCTAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2060.4559035078012615No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1940.4293460219099259No Hit
CCGCTAGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG1710.3784441739515326No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1650.3651654310058648No Hit
CCGCTAGGGGCCACAGCCATCTCCAGGACTGAATTATGTTCCAAAAAAAAGCATCCCC1510.33418169746597326No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1490.3297554498174173No Hit
CCGCTAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1450.32090295452030543No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1440.31868983069602747No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA1370.30319796392608167No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1370.30319796392608167No Hit
CCGCTAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA1320.2921323448046918No Hit
CCGCTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAGCATCGAAC1310.28991922098041384No Hit
CCGCTAGGGGGGGCTGGAGAGATGGTTCAATGGATAAGAGCACTGACTGCTCTTCCAA1300.2877060971561359No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1300.2877060971561359No Hit
CCGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1260.278853601859024No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1240.2744273542104681No Hit
CCGCTAGGGGGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAACCAGTTCTCGCC1230.27221423038619014No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA1120.24786986831913244No Hit
CCGCTAGGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTT1100.2434436206705765No Hit
CCGCTAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1050.23237800154918667No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA1050.23237800154918667No Hit
CCGCTAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG990.2190992586035189No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCC980.21688613477924087No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC980.21688613477924087No Hit
CCGCTAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT930.20582051565785106No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA920.2036073918335731No Hit
CCGCTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA910.2013942680092951No Hit
CCGCTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT900.19918114418501714No Hit
CCGCTAGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT870.19254177271218323No Hit
CCGCTAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA850.1881155250636273No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA840.18590240123934934No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT840.18590240123934934No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.18147615359079342No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC800.17704990594223746No Hit
CCGCTAGGGGCTCTTTTCCTCCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGACA790.1748367821179595No Hit
CCGCTAGGGGCTCCGCCGTCTGCCACTGCCGCCTGGGCTCACTGAGTGGTTCATCTGG770.17041053446940357No Hit
CCGCTAGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC770.17041053446940357No Hit
CCGCTAGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCCCCCCCTATCGG750.16598428682084762No Hit
CCGCTAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC750.16598428682084762No Hit
CCGCTAGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT710.15713179152373574No Hit
CCGCTAGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA700.1549186676994578No Hit
CCGCTAGGGGAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGATTGCTCTTCCAG690.15270554387517982No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA670.1482792962266239No Hit
CCGCTAGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGC650.14385304857806794No Hit
CCGCTAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG650.14385304857806794No Hit
CCGCTAGGGGGGAGGCCGGGGGAGAGACTCTGAACTTACCGAGTTTGCCCGCCGTGAT640.14163992475378998No Hit
CCGCTAGGGTAGAAGGGGGTTTTCTCAACCTCTGGCTGCTGAGAGCGATACTTCAGAA640.14163992475378998No Hit
CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA610.13500055328095606No Hit
CCGCTAGGGGAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTG610.13500055328095606No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC610.13500055328095606No Hit
CCGCTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG600.1327874294566781No Hit
CCGCTAGGGGAGCTGGCTGGTCGGGGACAGGAAAGAAGGAAGAACTAACTCAGATAAA600.1327874294566781No Hit
CCGCTAGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTT600.1327874294566781No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCC600.1327874294566781No Hit
CCGCTAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCA590.13057430563240013No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCGA590.13057430563240013No Hit
CCGCTAGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCCCCCCATATCGG590.13057430563240013No Hit
CCGCTAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAGAAGAAA590.13057430563240013No Hit
CCGCTAGGGAGCCAAAGCAACACCTCGCACTCCTGCCCCAGCATGCAGAAGGACGCCT570.1261480579838442No Hit
CCGCTAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA560.12393493415956622No Hit
CCGCTAGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT550.12172181033528826No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA540.11950868651101028No Hit
CCGCTAGGGGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA540.11950868651101028No Hit
CCGCTAGGGGAGTAATGGGCTCCACCCTTCCTTGCTCCCAGGGTATCAGAAGAACAGA540.11950868651101028No Hit
CCGCTAGGGGCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCACG540.11950868651101028No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC530.11729556268673233No Hit
CCGCTAGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC500.11065619121389841No Hit
CCGCTAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC500.11065619121389841No Hit
CCGCTAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGCCC490.10844306738962044No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCC490.10844306738962044No Hit
CCGCTAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG490.10844306738962044No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGTTCTTCCGA480.10622994356534249No Hit
CCGCTAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA470.10401681974106451No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA470.10401681974106451No Hit
CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC470.10401681974106451No Hit
CCGCTAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA470.10401681974106451No Hit
CCGCTAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA470.10401681974106451No Hit
CCGCTAGGGGGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCCCCCCCTATCGG470.10401681974106451No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCTGACTGCTCTTCTGA460.10180369591678655No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGGGCGGGGCCTTCTCCT460.10180369591678655No Hit
CCGCTAGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC460.10180369591678655No Hit
CCGCTAGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA460.10180369591678655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCCT150.007121181652.0998924
TAGCGCA353.7160135E-852.0998924
TTGCAAG307.720628E-752.0998923
TGGTAGA150.007121181652.0998926
TGACTCC150.007121181652.0998924
TTCCGCG353.7160135E-852.0998923
TGCCGCG150.007121181652.0998925
GCCTACA150.007121181652.0998927
CGGAAAA150.007121181652.0998922
AGGCCTA150.007121181652.0998925
AAAGGTC150.007121181652.0998926
AAGGTCC150.007121181652.0998927
CGCGGCA353.7160135E-852.0998926
AGCGCAG353.7160135E-852.0998925
AGGACTG203.3800176E-452.09988825
CACTGCC203.3800176E-452.09988824
CCACTGC203.3800176E-452.09988823
TGCCACT203.3800176E-452.09988821
GACTGAA203.3800176E-452.09988827
CAGGACT203.3800176E-452.09988824
Produced by FastQC (version 0.11.5)